scholarly journals Gene-expression changes induced by Feline immunodeficiency virus infection differ in epithelial cells and lymphocytes

2005 ◽  
Vol 86 (8) ◽  
pp. 2239-2248 ◽  
Author(s):  
R. J. O. Dowling ◽  
D. Bienzle
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Virus Infection ◽  
Feline Immunodeficiency Virus ◽  
Cell Types ◽  
Early Gene ◽  
Time Points ◽  
Subtype B ◽  
Immunodeficiency Virus ◽  
Transcriptional Changes

Infection of cats with Feline immunodeficiency virus (FIV) is an important model for understanding comparative lentivirus biology. In vivo, FIV infects lymphocytes and monocyte/macrophages, but in vitro infection is commonly investigated in epithelial Crandell–Reese Feline Kidney (CRFK) cells. In this study, the transcriptional responses of CRFK cells and primary lymphocytes to infection with FIV 34TF, a cloned subtype A virus, and FIV USgaB01, a biological subtype B isolate, were determined. Reverse-transcribed mRNA from both cell types was hybridized to microarrays containing 1700 human expressed sequence tags in duplicate and data were analysed with Significance Analysis of Microarrays (sam) software. Results from six experiments assessing homeostatic cross-species hybridization excluded 3·48 % inconsistently detected transcripts. Analysis of data from five time points over 48 h after infection identified 132 and 24 differentially expressed genes in epithelial cells and lymphocytes, respectively. Genes involved in protein synthesis, the cell cycle, structure and metabolism were affected. The magnitude of gene-expression changes ranged from 0·62 to 1·62 and early gene induction was followed by downregulation after 4 h. Transcriptional changes in CRFK cells were distinct from those in lymphocytes, except for heat-shock cognate protein 71, which was induced at multiple time points in both cell types. These findings indicate that FIV infection induces transcriptional changes of a modest magnitude in a wide range of genes, which is probably reflective of the relatively non-cytopathic nature of virus infection.

scholarly journals Persistent Gene Expression in Mouse Nasal Epithelia following Feline Immunodeficiency Virus-Based Vector Gene Transfer

2005 ◽  
Vol 79 (20) ◽  
pp. 12818-12827 ◽  
Author(s):  
Patrick L. Sinn ◽  
Erin R. Burnight ◽  
Melissa A. Hickey ◽  
Gary W. Blissard ◽  
Paul B. McCray
Keyword(s):  
Gene Expression ◽  
Gene Transfer ◽  
Epithelial Cells ◽  
Reporter Gene ◽  
Feline Immunodeficiency Virus ◽  
Envelope Protein ◽  
Envelope Glycoproteins ◽  
Airway Epithelia ◽  
Human Airway ◽  
Immunodeficiency Virus

ABSTRACT Gene transfer development for treatment or prevention of cystic fibrosis lung disease has been limited by the inability of vectors to efficiently and persistently transduce airway epithelia. Influenza A is an enveloped virus with natural lung tropism; however, pseudotyping feline immunodeficiency virus (FIV)-based lentiviral vector with the hemagglutinin envelope protein proved unsuccessful. Conversely, pseudotyping FIV with the envelope protein from influenza D (Thogoto virus GP75) resulted in titers of 106 transducing units (TU)/ml and conferred apical entry into well-differentiated human airway epithelial cells. Baculovirus GP64 envelope glycoproteins share sequence identity with influenza D GP75 envelope glycoproteins. Pseudotyping FIV with GP64 from three species of baculovirus resulted in titers of 107 to 109 TU/ml. Of note, GP64 from Autographa californica multicapsid nucleopolyhedrovirus resulted in high-titer FIV preparations (∼109 TU/ml) and conferred apical entry into polarized primary cultures of human airway epithelia. Using a luciferase reporter gene and bioluminescence imaging, we observed persistent gene expression from in vivo gene transfer in the mouse nose with A. californica GP64-pseudotyped FIV (AcGP64-FIV). Longitudinal bioluminescence analysis documented persistent expression in nasal epithelia for ∼1 year without significant decline. According to histological analysis using a LacZ reporter gene, olfactory and respiratory epithelial cells were transduced. In addition, methylcellulose-formulated AcGP64-FIV transduced mouse nasal epithelia with much greater efficiency than similarly formulated vesicular stomatitis virus glycoprotein-pseudotyped FIV. These data suggest that AcGP64-FIV efficiently transduces and persistently expresses a transgene in nasal epithelia in the absence of agents that disrupt the cellular tight junction integrity.

scholarly journals Alterations in RANTES Gene Expression and T-Cell Prevalence in Intestinal Mucosa during Pathogenic or Nonpathogenic Simian Immunodeficiency Virus Infection

Virology ◽  
1999 ◽  
Vol 259 (1) ◽  
pp. 110-118 ◽  
Author(s):  
Thomas Ndolo ◽  
Jeanette Rheinhardt ◽  
Melinda Zaragoza ◽  
Zeljka Smit-McBride ◽  
Satya Dandekar
Keyword(s):  
Gene Expression ◽  
T Cell ◽  
Virus Infection ◽  
Simian Immunodeficiency Virus ◽  
Intestinal Mucosa ◽  
Simian Immunodeficiency Virus Infection ◽  
Immunodeficiency Virus

Effect of primary-stage feline immunodeficiency virus infection on subsequent feline calicivirus vaccination and challenge in cats

AIDS ◽  
1991 ◽  
Vol 5 (6) ◽  
pp. 747-750 ◽  
Author(s):  
Susan Dawson ◽  
Neil R. Smyth ◽  
Malcolm Bennett ◽  
Rosalind M. Gaskell ◽  
Christine M. McCracken ◽  
...  
Keyword(s):  
Virus Infection ◽  
Feline Immunodeficiency Virus ◽  
Feline Calicivirus ◽  
Primary Stage ◽  
Feline Immunodeficiency Virus Infection ◽  
Immunodeficiency Virus

Phylogenetic characterisation of feline immunodeficiency virus in naturally infected cats in Croatia indicates additional heterogeneity of subtype B in Europe

2016 ◽  
Vol 161 (9) ◽  
pp. 2567-2573 ◽  
Author(s):  
Matko Perharić ◽  
Marina Biđin ◽  
Vilim Starešina ◽  
Zoran Milas ◽  
Nenad Turk ◽  
...  
Keyword(s):  
Feline Immunodeficiency Virus ◽  
Subtype B ◽  
Immunodeficiency Virus

scholarly journals Transcriptional Changes in the Transition from Vegetative Cells to Asexual Development in the Model Fungus Aspergillus nidulans

2012 ◽  
Vol 12 (2) ◽  
pp. 311-321 ◽  
Author(s):  
Aitor Garzia ◽  
Oier Etxebeste ◽  
Julio Rodríguez-Romero ◽  
Reinhard Fischer ◽  
Eduardo A. Espeso ◽  
...  
Keyword(s):  
Gene Expression ◽  
Aspergillus Nidulans ◽  
Cell Types ◽  
Asexual Development ◽  
Primary Metabolism ◽  
Air Exposure ◽  
Vegetative Cells ◽  
Content Type ◽  
Zinc Cluster ◽  
Transcriptional Changes

ABSTRACTMorphogenesis encompasses programmed changes in gene expression that lead to the development of specialized cell types. In the model fungusAspergillus nidulans, asexual development involves the formation of characteristic cell types, collectively known as the conidiophore. With the aim of determining the transcriptional changes that occur upon induction of asexual development, we have applied massive mRNA sequencing to compare the expression pattern of 19-h-old submerged vegetative cells (hyphae) with that of similar hyphae after exposure to the air for 5 h. We found that the expression of 2,222 (20.3%) of the predicted 10,943A. nidulanstranscripts was significantly modified after air exposure, 2,035 being downregulated and 187 upregulated. The activation during this transition of genes that belong specifically to the asexual developmental pathway was confirmed. Another remarkable quantitative change occurred in the expression of genes involved in carbon or nitrogen primary metabolism. Genes participating in polar growth or sexual development were transcriptionally repressed, as were those belonging to the HogA/SakA stress response mitogen-activated protein (MAP) kinase pathway. We also identified significant expression changes in several genes purportedly involved in redox balance, transmembrane transport, secondary metabolite production, or transcriptional regulation, mainly binuclear-zinc cluster transcription factors. Genes coding for these four activities were usually grouped in metabolic clusters, which may bring regulatory implications for the induction of asexual development. These results provide a blueprint for further stage-specific gene expression studies during conidiophore development.

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