WildTermitomycesSpecies Collected from Ondo and Ekiti States Are More Related to African Species as Revealed by ITS Region of rDNA

Molecular identification of eighteenTermitomycesspecies collected from two states, Ondo and Ekiti in Nigeria was carried out using the internal transcribed spacer (ITS) region. The amplicons obtained from rDNA ofTermitomycesspecies were compared with existing sequences in the NCBI GenBank. The results of the ITS sequence analysis discriminated between all theTermitomycesspecies (obtained from Ondo and Ekiti States) andTermitomycessp. sequences obtained from NCBI GenBank. The degree of similarity of T1 to T18 to gene ofTermitomycessp. obtained from NCBI ranges between 82 and 99 percent.Termitomycesspecies from Garbon with ascension number AF321374 was the closest relative of T1 to T18 except T12 that has T. eurhizus and T. striatus as the closet relative. Phylogenetic tree generated with ITS sequences obtained from NCBI GenBank data revealed that T1 to T18 are more related toTermitomycesspecies indigenous to African countries such as Senegal, Congo, and Gabon.

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Comparison of the ITS Sequences of 5 Common Potentilla Species in Jilin Province of China

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.554-556.1690 ◽

2012 ◽

Vol 554-556 ◽

pp. 1690-1693 ◽

Cited By ~ 2

Author(s):

Shao Xuan Zhang ◽

Xin Rui Liu ◽

Bo Chuan Wang ◽

Yun Hui Ling ◽

De Jun Sun ◽

...

Keyword(s):

Internal Transcribed Spacer ◽

Sequence Data ◽

Its Region ◽

Scientific Data ◽

Jilin Province ◽

Its Sequences ◽

Universal Primers ◽

Its Sequence ◽

Its Sequence Analysis ◽

Pcr Products

To find the differences in the internal transcribed spacer(ITS) sequences and provide scientific data for the authentication of Potentilla chinensis and its related species, we extracted the genome DNA from the leaves of 5 common Potetilla species in Jilin Province, amplified the ITS region using ITS universal primers of angiosperm, and sequenced the purified PCR products directly. Polymorphism of ITS sequences was found within P. chinensis and the sequence data suggested that our samples of this species might be related to hybridization. Other 4 species showed intraspecies-stability in ITS sequence. The ITS sequences of these 5 Potentilla species are significantly different. So ITS sequence analysis and other methods derived from it can be used in authentication of Potentilla.

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Internal transcribed spacer for identification of yeast species isolated from cancer patients at the Isotope and Radiation Center, Khartoum, Sudan: A cross-sectional, case-control study

F1000Research ◽

10.12688/f1000research.14019.1 ◽

2018 ◽

Vol 7 ◽

pp. 443 ◽

Cited By ~ 1

Author(s):

Mohamed M.A. Nagla ◽

Omer E. El Fadil ◽

Abdel Hamid M. Muzamil ◽

Altayeb N. Hisham ◽

Mohamed B. Bahaeldeen ◽

...

Keyword(s):

Sequence Analysis ◽

Cancer Patients ◽

Internal Transcribed Spacer ◽

Yeast Species ◽

Reference Sequence ◽

Positive Sequence ◽

Its Sequence ◽

Control Groups ◽

Its Sequence Analysis ◽

And Control

Background:Cancer patients have a high risk of fungal infections, especially by Candida species.Non-C. albicans Candidainfections and less common yeast infections have been increasing in recent years. Identification by conventional methods can be difficult and sometimes inconclusive. This study aimed to detect the prevalence of oral yeast species isolated from cancer patients, from oral swab, sputum and urine, using Internal Transcribed Spacer (ITS) sequence analysis, since little is known about this problem in Sudan.Methods:The study involved 333 cancer patients (168 patients under treatment [study group] and 165 patients before treatment [control group]). Oral swabs were collected from all patients. Urine or sputum specimens were collected from patients under treatment showing clinical features of UTI or lower respiratory tract infection, respectively. ITS1 and ITS2 region of isolated yeast were amplified by PCR and sequenced. The obtained sequences were compared to reference sequence available in the GenBank database using BLAST.Results:Culture results showed oral yeastspecies were isolated from 69/168 (41.1%) and 74/165 (44.8%) of patients among study and control groups, respectively (P value > 0.05). 2/9 (22.2%) patients were urine growth positive and 8/14 (57.1%) patients were sputum culture positive. Sequence analysis showed,C. albicanswas the most prevalent organism (93; 52.5%) followed byC. tropicalis(29; 16.4%), andC. glabrata(24; 13.6%). Non-C. albicans Candidaand uncommon rare yeast were found to be associated with oral infections and colonization among the study and control groups, whereasC.albicans was the most common species (66.7%) associated with oral candidiasis among the treated patients. Conclusion:Cancer patients were highly colonized with different oral yeast species, which indicates that ITS sequence analysis is an accurate method for identification. This will aid effective management to prevent dissemination of disease especially among those who are under chemo and/or radiotherapy treatment.

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Characterization of Metarhizium anisopliae using amplifed ribossomal DNA restriction analysis (ARDRA) and internal transcribed spacer (ITS) sequence analysis

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb12.906 ◽

2012 ◽

Vol 11 (101) ◽

pp. 16635-16639

Author(s):

Maria do Carmo Catanho Pereira de Lyra ◽

◽

Maria Luiza Ribeiro Bastos da Silva ◽

Vanildo Alberto Leal Bezerra Cavalcanti ◽

Adália Cavalcanti Espírito Santo Mergulhão

Keyword(s):

Sequence Analysis ◽

Metarhizium Anisopliae ◽

Internal Transcribed Spacer ◽

Restriction Analysis ◽

Its Sequence ◽

Its Sequence Analysis ◽

Dna Restriction

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Phylogenetic relationships of selected Sri Lankan Orchids based on Internal Transcribed Spacer (ITS) sequence analysis

Tropical Plant Research ◽

10.22271/tpr.2020.v7.i1.011 ◽

2020 ◽

Vol 7 (1) ◽

pp. 76-85

Author(s):

P. M. H. Sandamali ◽

◽

S. P. Senanayake ◽

Sanath Rajapakse ◽

◽

...

Keyword(s):

Sequence Analysis ◽

Phylogenetic Relationships ◽

Internal Transcribed Spacer ◽

Its Sequence ◽

Sri Lankan ◽

Its Sequence Analysis

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ITS sequence analysis used for molecular identification of the Bupleurum species from northwestern China

Phytomedicine ◽

10.1016/j.phymed.2007.04.009 ◽

2007 ◽

Vol 14 (6) ◽

pp. 416-423 ◽

Cited By ~ 24

Author(s):

Zhi-Ye Yang ◽

Zhi Chao ◽

Ke-Ke Huo ◽

Hui Xie ◽

Zhi-Peng Tian ◽

...

Keyword(s):

Sequence Analysis ◽

Molecular Identification ◽

Northwestern China ◽

Its Sequence ◽

Its Sequence Analysis

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Screening and Identification of β-Glucosidase Producing Strain of Fusarium Oxysporum

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.652-654.402 ◽

2013 ◽

Vol 652-654 ◽

pp. 402-405

Author(s):

Jie Chen ◽

De Fang Dai ◽

Xiu Ling Gu ◽

Lin Yuan

Keyword(s):

Sequence Analysis ◽

Fusarium Oxysporum ◽

Internal Transcribed Spacer ◽

Morphological Features ◽

Its Sequence ◽

Its Sequence Analysis ◽

Screening And Identification

Five strains, which can produce β-glucosidase, were screened out from 96 strains isolated from linseed from different regions in China. Based on morphological features and internal transcribed spacer (ITS) sequence analysis, the five strains were all determined as Fusarium oxysporum. The strain named as S15 produces the highest β-glucosidase, which activity reach at 13.58 U/mL.

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Higher-level relationships among the eucalypts are resolved by ITS-sequence data

Australian Systematic Botany ◽

10.1071/sb00039 ◽

2002 ◽

Vol 15 (1) ◽

pp. 49 ◽

Cited By ~ 82

Author(s):

Dorothy A. Steane ◽

Dean Nicolle ◽

Gay E. McKinnon ◽

René E. Vaillancourt ◽

Brad M. Potts

Keyword(s):

Internal Transcribed Spacer ◽

Sequence Data ◽

Its Region ◽

Molecular Data ◽

Nuclear Ribosomal Dna ◽

Its Sequence ◽

Phylogenetic Groups ◽

Single Section ◽

Western Australian ◽

Genus Eucalyptus

This expanded survey of ITS sequences represents the largest analysis of molecular data ever attempted on Eucalyptus. Sequences of the internal transcribed spacer (ITS) region of the nuclear ribosomal DNA were included in an analysis of 90 species of Eucalyptus s.s. and 28 species representing eight other genera (Allosyncarpia, Angophora, Arillastrum, Corymbia, Eucalyptopsis, Stockwellia, Lophostemon and Metrosideros). The results of the study indicate that Angophora and Corymbia form a well-supported clade that is highly differentiated from Eucalyptus s.s. Corymbia species are divided between two clades, one of which may be the sister to Angophora. Allosyncarpia, Arillastrum, Eucalyptopsis and ‘Stockwellia’ are also highly differentiated from Eucalyptus s.s. If the genus Eucalyptus is to be expanded to include Angophora and Corymbia(sensu Brooker 2000), ITS data suggest that Allosyncarpia, Eucalyptopsis, ‘Stockwellia’ and potentially Arillastrum should also be included in Eucalyptus s.l. The ITS data suggest that subg. Symphyomyrtus is paraphyletic and that subg. Minutifructus should be included within it. Within subg.Symphyomyrtus, only sect. Maidenaria appears to be monophyletic. Sections Adnataria and Dumaria are probably monophyletic; sections Exsertaria and Latoangulatae are very close and probably should be combined in a single section. Section Bisectae is polyphyletic and is divided into two distinct lineages. The phylogenetic groups depicted by ITS data are consistent with the frequency of natural inter-specific hybridisations as well as data from controlled crosses within subgenus Symphyomyrtus. The ITS data illustrate that subg. Idiogenes and western Australian monocalypts are early evolutionary lines relative to E. diversifolia, E. rubiginosa (monotypic subg. Primitiva) and the eastern monocalypts and that subg. Primitiva should be sunk into subg. Eucalyptus. Subgenus Eudesmia may be monophyletic, grouping with subgenera Idiogenes and Eucalyptus. Further work is required to confirm the phylogenetic positions of the monotypic subgenera Alveolata, Cruciformes, Acerosae and Cuboidea.

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