scholarly journals Application of ATP Bioluminescence Assay for Measurement of Microbial Contamination in Fresh-cut Produce Processing Lines

2012 ◽  
Vol 19 (1) ◽  
pp. 62-66 ◽  
Author(s):  
Ji-Weon Choi ◽  
Hye-Eun Lee ◽  
Chang-Kug Kim ◽  
Won-Bae Kim ◽  
Ji-Kang Kim
Keyword(s):  
Microbial Contamination ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Fresh Cut ◽  
Atp Bioluminescence Assay

Design and Preliminary Testing of Novel Injection Port Contamination Barrier Devices

2017 ◽  
Vol 11 (3) ◽  
Author(s):  
David B. Wax ◽  
Bryan Hill
Keyword(s):  
Adenosine Triphosphate ◽  
Microbial Contamination ◽  
Postoperative Infections ◽  
Protective Shell ◽  
Injection Port ◽  
Preliminary Testing ◽  
Environmental Surfaces ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Atp Bioluminescence Assay

Prior studies have linked microbial contamination of intravenous (IV) ports and stopcocks with postoperative infections. Existing technologies to address contamination are not consistently utilized because of the time and effort they require. Herein, novel barrier devices were created that form a protective shell to passively prevent contact between injection sites and practitioner hands or environmental surfaces while still allowing rapid connection of a syringe for injection of medications via an opening in the shell. Prototypes were tested using a grossly contaminated environment and adenosine triphosphate (ATP)-bioluminescence assay. For eight pairs of unshielded versus shielded IV ports/stopcocks, average contamination was 4102 versus 35 RLU (p < 0.02), respectively, indicating that the devices could significantly reduce IV port/stopcock contamination.

Comparison of a Rapid ATP Bioluminescence Assay and Standard Plate Count Methods for Assessing Microbial Contamination of Consumers' Refrigerators

2006 ◽  
Vol 69 (10) ◽  
pp. 2534-2538 ◽  
Author(s):  
FUR-CHI CHEN ◽  
SANDRIA L. GODWIN
Keyword(s):  
Microbial Contamination ◽  
Field Studies ◽  
Plate Count ◽  
Culture Methods ◽  
Psychrotrophic Bacteria ◽  
Standard Plate Count ◽  
Plate Count Agar ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Atp Bioluminescence Assay

The feasibility of using an ATP bioluminescence assay for assessing microbial contamination of home refrigerators was evaluated and compared with the standard culture methods. Samples of refrigerator surfaces were collected from 123 households by swabbing an area of 100 cm2 on three locations in the refrigerator with premoisturized sterile swabs. Microbial contaminations were determined by aerobic plate count (APC; incubated at 35°C for 48 h) and psychrotrophic plate count (PPC; incubated at 7°C for 10 days) on plate count agar. The results were compared to the readings from the microbial ATP (mATP) bioluminescence assay. The correlation coefficient (r) between mATP and PPC (r = 0.851) was slightly higher than that between mATP and APC (r = 0.823). Our results indicated a potential discrepancy in the population of mesophilic and psychrotrophic bacteria in the refrigerator samples. Nevertheless, mATP appeared to be a reliable indication of the average of APC and PPC (r = 0.895). The mATP bioluminescence assay would provide a rapid and convenient test for researchers in field studies to assess microbial contamination in refrigerators.

scholarly journals ATP-bioluminescence assay as an alternative for hygiene-monitoring procedures of stainless steel milk contact surfaces

2006 ◽  
Vol 37 (3) ◽  
pp. 345-349 ◽  
Author(s):  
Patrícia Dolabela Costa ◽  
Nélio José Andrade ◽  
Sebastião César Cardoso Brandão ◽  
Frederico José Vieira Passos ◽  
Nilda de Fátima Ferreira Soares
Keyword(s):  
Stainless Steel ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Contact Surfaces ◽  
Atp Bioluminescence Assay

Detection of fungi and control of disinfection by ATP-bioluminescence assay

2003 ◽  
Vol 28 (1) ◽  
pp. 16-22 ◽  
Author(s):  
Malalanirina Rakotonirainy ◽  
Jozef Hanus ◽  
Sylvette Bonassies-Termes ◽  
Cécile Heraud ◽  
Bertrand Lavédrine
Keyword(s):  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Atp Bioluminescence Assay ◽  
And Control
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