scholarly journals Production of D-fructose from starch by amylase and glucose isomerase

2021 ◽  
Vol 28 (5) ◽  
pp. 674-684
Author(s):  
Sang Jin Kim ◽  
Ji In Choi ◽  
Min Su Choi ◽  
Chang-Su Park
Keyword(s):  
1970 ◽  
Vol 1 ◽  
pp. 6-10 ◽  
Author(s):  
Bidur Dhungel ◽  
Manoj Subedi ◽  
Kiran Babu Tiwari ◽  
Upendra Thapa Shrestha ◽  
Subarna Pokhrel ◽  
...  

Glucose isomerase (EC 5.3.1.5) was extracted from Streptomyces spp., isolated from Mt. Everest soil sample, and purified by ammonium sulfate fractionation and Sepharose-4B chromatography. A 7.1 fold increase in specific activity of the purified enzyme over crude was observed. Using glucose as substrate, the Michaelis constant (KM<) and maximal velocity (Vmax) were found to be 0.45M and 0.18U/mg. respectively. The optimum substrate (glucose) concentration, optimum enzyme concentration, optimum pH, optimum temperature, and optimum reaction time were 0.6M, 62.14μg/100μl, 6.9, 70ºC, and 30 minutes, respectively. Optimum concentrations of Mg2+ and Co2+ were 5mM and 0.5mM, respectively. The enzyme was thermostable with half-life 30 minutes at 100ºC.DOI: 10.3126/ijls.v1i0.2300 Int J Life Sci 1 : 6-10


1978 ◽  
pp. 415-416
Author(s):  
A. Rosevear ◽  
C. A. Kent ◽  
A. R. Thomson ◽  
C. Bucke

1991 ◽  
Vol 26 (4) ◽  
pp. 444-447
Author(s):  
B. A. Tashpulatova ◽  
Zh. Kh. Dierov ◽  
K. D. Davranov

2011 ◽  
Vol 47 (2) ◽  
pp. 151-157 ◽  
Author(s):  
G. A. Kovalenko ◽  
L. V. Perminova ◽  
T. V. Chuenko ◽  
L. I. Sapunova ◽  
E. A. Shlyakhotko ◽  
...  

2008 ◽  
Vol 64 (a1) ◽  
pp. C372-C373
Author(s):  
C. Besnard ◽  
F. Gramiccia ◽  
Y. Pellegrinelli ◽  
S. Basso ◽  
M. Schiltz

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