scholarly journals OBERON3 and SUPPRESSOR OF MAX2 1-LIKE proteins form a regulatory module specifying phloem identity

2019 ◽  
Author(s):  
Eva-Sophie Wallner ◽  
Nina Tonn ◽  
Friederike Wanke ◽  
Vadir Lopéz-Salmerón ◽  
Michael Gebert ◽  
...  

AbstractThe phloem tissue mediates long-distance transport of energy metabolites along plant bodies and, therefore, is central for plant performance. However, mechanisms initiating the transition of undifferentiated stem cells to cells specialized in metabolite transport are unknown. Here we identify the ubiquitously expressed PHD-finger protein OBERON3 (OBE3) to be essential for phloem formation. We show that OBE3 directly interacts with the SUPPRESSOR OF MAX2 1-LIKE 5 (SMXL5) protein specifically expressed during early phloem development. Both proteins co-localize in nuclei of phloem stem cells and, together with the SMXL5 homologs SMXL3 and SMXL4, promote the establishment of phloem-specific cellular signatures in a cell-autonomous manner. These signatures include expression of OCTOPUS (OPS), BREVIS RADIX (BRX), BARELY ANY MERISTEM3 (BAM3), and COTYLEDON VASCULAR PATTERN2 (CVP2) genes acting as mediators of phloem differentiation. Consistently, genetic analyses show that SMXL5 acts upstream and independently of OPS and BRX functions. Based on our findings, we conclude that the formation of an OBE3/SMXL5 protein complex specifically in nuclei of early phloem cells is essential for establishing a phloem-specific developmental program.

Author(s):  
James Cronshaw

Long distance transport in plants takes place in phloem tissue which has characteristic cells, the sieve elements. At maturity these cells have sieve areas in their end walls with specialized perforations. They are associated with companion cells, parenchyma cells, and in some species, with transfer cells. The protoplast of the functioning sieve element contains a high concentration of sugar, and consequently a high hydrostatic pressure, which makes it extremely difficult to fix mature sieve elements for electron microscopical observation without the formation of surge artifacts. Despite many structural studies which have attempted to prevent surge artifacts, several features of mature sieve elements, such as the distribution of P-protein and the nature of the contents of the sieve area pores, remain controversial.


1999 ◽  
Vol 47 (4) ◽  
pp. 245-250 ◽  
Author(s):  
Ishwar D. Garg ◽  
S.M. Paul Khurana

The tospovirus isolate on tomato was found to be systemic while the one on potato was non-systemic. The virus was present in all the tissues except xylem vessels in tomato, while it was often found only in the cortical cells of potato stems. Virions were detected in all cells of phloem tissue in tomato, while none were present in the case of potato. Plasmodesmata were present between phloem parenchyma and the phloem sieve tubes in infected tomato but none were present in infected potato. There were pronounced plasmodesmal changes in response to infection in tomato. These included dissolution of the desmotubule (central rod-like structure), making the plasmodesmata pore-like structures with a diameter of ca. 45 nm, which contained electron-dense material, presumably ribonucleoprotein of the virus. No such changes were found in infected potato. These results of comparative studies suggested that the virus was non-systemic in potato due to its non-loading into the phloem and consequently lacked long distance transport.


Energies ◽  
2021 ◽  
Vol 14 (6) ◽  
pp. 1689
Author(s):  
Tomasz Neumann

The subject of the article is a comparative long-distance transport analysis based on the relationship between central and eastern China and Poland. It provides an overview of issues related to long-haul China–Poland. The technique for order of preference by similarity to ideal solution (TOPSIS) method was proposed in the multi-criteria analysis. This method was briefly discussed, and its choice was justified. Then, the criteria adopted in the analysis were presented, i.e., time, cost, maximum number of containers, and ecology index. Multi-criteria analysis was carried out for three cases: the transport of one loading unit, 82 loading units, and 200 loading units. The geopolitical and operational situation on the transport route for the analyzed modes of transport was discussed.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
T. Morel-Journel ◽  
E. Vergu ◽  
J.-B. Mercier ◽  
N. Bareille ◽  
P. Ezanno

AbstractThe transport of weaned calves from cow–calf producers to fatteners is a general concern for the young bull industry due to its documented negative impact on the welfare, health and performance of the animals. These transfers are often managed by intermediaries who transport weaned calves to sorting centres, where they are grouped into batches before being sent to fattening units. In this study, we present an algorithm to limiting these transfer distances by appropriately selecting the sorting centre through which they must go. We tested the effectiveness of this algorithm on historical data from a French beef producer organization managing 136,892 transfers using 13 sorting centres. The results show a decrease in the transfer distances compared to the historical record, especially for the calves travelling over long distances (− 76 km, i.e. 18% on average for the 33% longest transfers). Moreover, the distribution of calves between the sorting centres proposed by the algorithm reveals differences in their efficiency in minimizing transfer distances. In addition to its usefulness as a management tool for the daily transport of cattle, this algorithm provides prospects for improving the management of the sorting centres themselves.


2008 ◽  
Vol 45 (9) ◽  
pp. 1061-1082 ◽  
Author(s):  
Ryan C. McKellar ◽  
Alexander P. Wolfe ◽  
Ralf Tappert ◽  
Karlis Muehlenbachs

The Late Cretaceous Grassy Lake and Cedar Lake amber deposits of western Canada are among North America’s most famous amber-producing localities. Although it has been suggested for over a century that Cedar Lake amber from western Manitoba may be a secondary deposit having originated from strata in Alberta, this hypothesis has not been tested explicitly using geochemical fingerprinting coupled to comparative analyses of arthropod faunal content. Although there are many amber-containing horizons associated with Cretaceous coals throughout Alberta, most are thermally mature and brittle, thus lacking the resilience to survive long distance transport while preserving intact biotic inclusions. One of the few exceptions is the amber found in situ at Grassy Lake. We present a suite of new analyses from these and other Late Cretaceous ambers from western Canada, including stable isotopes (H and C), Fourier transform infrared (FTIR) spectra, and an updated faunal compendium for the Grassy and Cedar lakes arthropod assemblages. When combined with amber’s physical properties and stratigraphic constraints, the results of these analyses confirm that Cedar Lake amber is derived directly from the Grassy Lake amber deposit or an immediate correlative equivalent. This enables the palaeoenvironmental context of Grassy Lake amber to be extended to the Cedar Lake deposit, making possible a more inclusive survey of Cretaceous arthropod faunas.


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