scholarly journals Initial evaluation of a mobile SARS-CoV-2 RT-LAMP testing strategy

Author(s):  
Christina M Newman ◽  
Dawn M Dudley ◽  
Roger W Wiseman ◽  
Matthew T McLaughlin ◽  
Julie A Karl ◽  
...  

SARS-CoV-2 control in the United States is hampered by limited testing. We evaluated a simple, outdoor, mobile RT-LAMP assay workflow where self-collected saliva is tested for SARS-CoV-2 RNA. 494 volunteers were tested over 16 days. Testing results were 99.8% concordant with qRT-PCR, with all but two people testing SARS-CoV-2-negative.

2015 ◽  
Vol 53 (9) ◽  
pp. 2983-2989 ◽  
Author(s):  
Michelle Dupuis ◽  
Scott Brunt ◽  
Kim Appler ◽  
April Davis ◽  
Robert Rudd

Rabies virus found worldwide and prevalent throughout the United States continues to be a public health concern. Direct-fluorescent antibody (DFA) detection remains the gold standard for rabies virus diagnostics. Assessing the utility of a high-throughput molecular platform such as the QIAsymphony SP/AS, in conjunction with quantitative reverse transcription-PCR (qRT-PCR), to augment or potentially replace the DFA test, was the focus of this project. Here we describe a triplex qRT-PCR assay, including assembly and evaluation for sensitivity, specificity, and ability to detect variants. Additionally, we compared the qRT-PCR assay to the gold standard direct fluorescent-antibody test. More than 1,000 specimens submitted for routine rabies diagnosis were tested to directly compare the two methods. All results were in agreement between the two methods, with one additional specimen detected by qRT-PCR below the limits of the DFA sensitivity. With the proper continued validation for variant detection, molecular methods have a place in routine rabies diagnostics within the United States.


2020 ◽  
Author(s):  
Benjamin Lengerich ◽  
Willie Neiswanger ◽  
Eugene J. Lengerich ◽  
Eric P. Xing

AbstractTo design effective disease control strategies, it is critical to understand the incidence of diseases. In the Covid-19 epidemic in the United States (caused by outbreak of the SARS-CoV-2 virus), testing capacity was initially very limited and has been increasing at the same time as the virus has been spreading. When estimating the incidence, it can be difficult to distinguish whether increased numbers of positive tests stem from increases in the spread of the virus or increases in testing. This has made it very difficult to identify locations in which the epidemic poses the largest public health risks. Here, we use a probabilistic model to quantify beliefs about testing strategies and understand implications regarding incidence. We apply this model to estimate the incidence in each state of the United States, and find that: (1) the Covid-19 epidemic is likely to be more widespread than reported by limited testing, (2) the Covid-19 epidemic growth in the summer months is likely smaller than it was during the spring months, and (3) the regions which are at highest risk of Covid-19 epidemic outbreaks are not always those with the largest number of positive test results.


Author(s):  
Josephina A. Vossen

Chapter 35 discusses gout, a metabolic disorder in which hyperuricemia leads to deposition of monosodium urate (MSU) crystals in soft tissues and joints. Gout is the most common crystalline type of arthropathy in the United States. Gout is considered to have 4 phases, characterized by asymptomatic hyperuricemia, recurrent attacks of acute arthritis, intercritical gout and chronic tophaceous gout. Radiography is generally used in the initial evaluation of gouty arthritis. Characteristic of gout are well-defined, punched-out erosions with overhanging edges, with late preservation of the joint space, lack of periarticular osteopenia, asymmetrical involvement, and soft tissue nodules that may calcify.


2017 ◽  
Vol 36 (1) ◽  
pp. 1-15 ◽  
Author(s):  
Keith A. Anderson ◽  
Kate P. Chapin ◽  
Zachary Reimer ◽  
Gina Siffri

Subject Vietnam's handling of COVID-19. Significance The ruling Communist Party of Vietnam (CPV) has considerably relaxed the country's COVID-19 lockdown, within just three weeks of imposing it. Vietnam is the most populous country to have reported no deaths from the disease, although its strikingly low number of confirmed cases may reflect limited testing. Impacts Vietnam will try to present its apparent success in managing the pandemic as a vindication of its one-party system. Hanoi will likely announce more packages of economic support for businesses and vulnerable individuals. Vietnam will have less scope to export to Europe and the United States, where recovery from the pandemic looks set to be slower.


2020 ◽  
Vol 50 (6-7) ◽  
pp. 519-525 ◽  
Author(s):  
Mark J. Rozell ◽  
Clyde Wilcox

This article compares and contrasts the responses of Australia, Canada, Germany, and the United States to the COVID-19 outbreak and spread. The pandemic has posed special challenges to these federal systems. Although federal systems typically have many advantages—they can adapt policies to local conditions, for example, and experiment with different solutions to problems—pandemics and people cross regional borders, and controlling contagion requires a great deal of national coordination and intergovernmental cooperation. The four federal systems vary in their relative distribution of powers between regional and national governments, in the way that health care is administered, and in the variation in policies across regions. We focus on the early responses to COVID-19, from January through early May 2020. Three of these countries—Australia, Canada, and Germany—have done well in the crisis. They have acted quickly, done extensive testing and contact tracing, and had a relatively uniform set of policies across the country. The United States, in contrast, has had a disastrous response, wasting months at the start of the virus outbreak, with limited testing, poor intergovernmental cooperation, and widely divergent policies across the states and even within some states. The article seeks to explain both the relative uniform responses of these three very different federal systems, and the sharply divergent response of the United States.


2019 ◽  
Vol 16 (11) ◽  
pp. 1522-1527 ◽  
Author(s):  
Jina Pakpoor ◽  
Micheal Raad ◽  
Andrew Harris ◽  
Varun Puvanesarajah ◽  
Joseph K. Canner ◽  
...  

2020 ◽  
Vol 58 (11) ◽  
Author(s):  
Matthew A. Pettengill ◽  
Alexander J. McAdam

ABSTRACT Frequent, low-cost, universal testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with quarantine of those with a positive result has been suggested as a strategy to address the coronavirus disease 2019 (COVID-19) pandemic in the United States. Specifically, home or community use of tests that use paper strip detection devices, which may have reduced sensitivity for SARS-CoV-2, has been advocated. There are several potential challenges or problems with this strategy, including the limited availability of such tests, consequences of incorrect test results, difficulties with adherence to testing, and the questionable accuracy of such tests for detection of infectious people. Because of these, we think it is premature to strongly advocate for such a testing strategy, as the adverse consequences may outweigh any benefits. High-quality outcome data demonstrating the efficacy of this testing strategy are needed before widespread implementation.


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