scholarly journals Basic reproduction numbers of three strains of mouse hepatitis viruses in mice

2021 ◽  
Author(s):  
Masataka Nakayama ◽  
Shigeru Kyuwa
Keyword(s):  
Gastrointestinal Tract ◽  
Immunosorbent Assay ◽  
Hepatitis Virus ◽  
Mouse Hepatitis Virus ◽  
Laboratory Animals ◽  
Enzyme Linked Immunosorbent Assay ◽  
Laboratory Mice ◽  
Hepatitis Viruses ◽  
Viral Growth ◽  
Reproduction Numbers

Mouse hepatitis virus (MHV) is a murine coronavirus and one of the most important pathogens in laboratory mice. Although various strains of MHV have been isolated, they are generally excreted in the feces and transmitted oronasally via aerosols and contaminated bedding. In this study, we attempted to determine the basic reproduction numbers of three strains of MHV to improve our understanding of MHV infections in mice. Five-week-old female C57BL/6J mice were inoculated intranasally with either the Y, NuU, or JHM variant strain of MHV and housed with two naive mice. After 4 weeks, the presence or absence of anti-MHV antibody in the mice was determined by the enzyme-linked immunosorbent assay. We also examined the distribution of MHV in the organs of Y, NuU, or JHM variant-infected mice. Our data suggest that the transmissibility of MHV is correlated with viral growth in the gastrointestinal tract of infected mice. To the best of our knowledge, this is the first report to address the basic reproduction numbers among pathogens in laboratory animals.

scholarly journals RT-PCR Assay for Detection of Enterotropic Strains of Mouse Hepatitis Virus in Faecal Samples

2021 ◽  
Author(s):  
M.R. Srinivasan ◽  
K. Vijay ◽  
A.K. Karuppannan ◽  
S. Ramesh ◽  
Y. Krishnamohan Reddy
Keyword(s):  
Viral Infections ◽  
Hepatitis Virus ◽  
Mouse Hepatitis Virus ◽  
N Gene ◽  
Laboratory Mice ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Faecal Samples ◽  
Retrospective Nature ◽  
Gene Template

Background: Mouse Hepatitis Virus (MHV) is one of the most important and common viral infections of laboratory mice, due to its highly contagious and subclinical nature, posing threat to the research outcomes. Periodic screening of laboratory mice for MHV is mandatory. Hence, this study was intended to develop sensitive faecal based RT-PCR assays to detect active infection of enterotropic MHV in laboratory mice. Methods: Primers targeting N-gene of MHV were selected and their sensitivity was analysed in tenfold serially diluted gene template in the presence of negative mouse faecal cDNA. Thirty-six weaned mice at the age of 6 to 18 weeks were randomly selected at different periods and the blood and faecal sample were collected for serology and RT-PCR assay respectively. RT-PCR assay in colon samples was carried out for comparison. Result: PCR assay of MHV detected as low as 4 fg of plasmid DNA. Seroprevalence of MHV is very high than the prevalence by RT-PCR assay showing its retrospective nature and also seroprevalence includes both enterotropic and polytropic strain. RT-PCR results in faecal samples are analogous with that of the colon samples, showing the reliability of antemortem testing of mice for enterotropic strain of MHV.

Enzyme-linked immunosorbent assay for detection of antibodies to murine hepatitis virus.

1979 ◽  
Vol 10 (4) ◽  
pp. 595-597 ◽  
Author(s):  
R L Peters ◽  
M J Collins ◽  
A J O'Beirne ◽  
P A Howton ◽  
S L Hourihan ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Hepatitis Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Murine Hepatitis Virus

scholarly journals Evaluation of home-mode ELISA system for thedetection of antibodies against Escherichia coli O157:H7 using purified lipopolysaccharide

2008 ◽  
Vol 5 (4) ◽  
pp. 563-568
Author(s):  
Baghdad Science Journal
Keyword(s):  
Escherichia Coli ◽  
Immunosorbent Assay ◽  
Gel Filtration ◽  
Laboratory Animals ◽  
Partial Purification ◽  
Nucleic Acid Content ◽  
Enzyme Linked Immunosorbent Assay ◽  
Biochemical Basis ◽  
Elisa System ◽  
E Coli

An enzyme linked immunosorbent assay (ELISA) for the detection and quantitation of human immunoglobulin G (IgG) antibodies against vero- cytotoxine (VT) producing Escherichia coli serogroup O157:H7 was produced. E. coli O157: H7 lipopolysaccharide was extracted from locally isolated strains by using hot phenol- water method, followed by partial purification using gel filtration chromatography by sepharose- 4B. The purity of the lipopolysaccharide was checked by measuring the protein and nucleic acid content and then used as antigen. Four isolates of vero- cytotoxin producing E. coli serogroup O157:H7 was obtained by culturing 350 stool samples from children suffering from bloody diarrhea. These isolates were identified on bacteriological, serological and biochemical basis. Toxin production was confirmed on laboratory animals as well as by cytopathic effect on tissue culture. The possibility of using E. coli O157:H7 lipopolysaccharide in an enzyme- linked immunosorbent assay for the routine diagnostic testing of serum from patients for evidence of O157:H7 infection is discussed.

Studies on the Detection of Antibody to Duck Hepatitis Virus by Enzyme-Linked Immunosorbent Assay

1991 ◽  
Vol 35 (4) ◽  
pp. 778 ◽  
Author(s):  
Xinliu Zhao ◽  
R. M. Phillips ◽  
Guangdi Li ◽  
Anqing Zhong
Keyword(s):  
Immunosorbent Assay ◽  
Hepatitis Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Duck Hepatitis Virus ◽  
Duck Hepatitis

scholarly journals An enzyme-linked immunosorbent assay for the detection of mouse polyomavirus-specific antibodies in laboratory mice

1994 ◽  
Vol 28 (3) ◽  
pp. 257-261 ◽  
Author(s):  
H. W. J. Broeders ◽  
J. Groen ◽  
G. van Steenis ◽  
A. D. M. E. Osterhaus
Keyword(s):  
Immunosorbent Assay ◽  
Haemagglutination Inhibition ◽  
Immunofluorescence Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Pathogen Free ◽  
Laboratory Mice ◽  
Serum Samples ◽  
Laboratory Rodents ◽  
Antibody Titres ◽  
Specific Pathogen

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection and quantification of IgM and IgG serum antibodies to mouse polyomavirus (MPV). To evaluate the potential of this ELISA for the screening of laboratory rodents, serum samples from specific pathogen free (SPF) BALB/c RIVM mice, collected after experimental intraperitoneal infection with MPV, were tested by this assay. The results were compared with those obtained from the same sera in an immunofluorescence assay (IFA) and a haemagglutination inhibition assay (HIA). The ELISA proved to be the most sensitive of the 3 assays, allowing the detection of seropositive animals within 7 days post-infection and giving antibody titres that were about 4 to 8 times higher than those found in the IFA and HIA respectively. More than 5000 serum samples from non-infected specific pathogen free laboratory mice and 90 from 10 SPF N:NIH/RIVM mice experimentally infected with K-papovavirus, were negative in this assay, thus confirming the specificity of the ELISA

scholarly journals Simultaneous Detection of Antibodies to Mouse Hepatitis Virus Recombinant Structural Proteins by a Microsphere-Based Multiplex Fluorescence Immunoassay

2011 ◽  
Vol 18 (5) ◽  
pp. 758-766 ◽  
Author(s):  
Satoshi Kunita ◽  
Kanako Kato ◽  
Miyuki Ishida ◽  
Kozue Hagiwara ◽  
Shuko Kameda ◽  
...  
Keyword(s):  
False Positive ◽  
Hepatitis Virus ◽  
Fluorescent Antibody ◽  
Laboratory Mouse ◽  
False Negative ◽  
Mouse Hepatitis Virus ◽  
Simultaneous Detection ◽  
False Negative Rate ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples

ABSTRACTWe describe a new microsphere-based multiplex fluorescent immunoassay (MFI) using recombinant mouse hepatitis virus (MHV) proteins to detect antibodies to coronaviruses in mouse and rat sera. All the recombinant proteins, including nucleocapsid (N) and 3 subunits of spike protein, S1, S2, and Smid, showed positive reactivity in MFI with mouse antisera to 4 MHV strains (MHV-S, -A59, -JHM, and -Nu67) and rat antiserum to a strain of sialodacryoadenitis virus (SDAV-681). The MFI was evaluated for its diagnostic power, with panels of mouse sera classified as positive or negative for anti-MHV antibodies by enzyme-linked immunosorbent assay (ELISA) using MHV virion antigen and indirect fluorescent antibody assay. The reactivities of 236 naturally infected mouse sera were examined; 227 samples were positive by MFI using S2 antigen (96% sensitivity), and 208 samples were positive using N antigen (88% sensitivity). Based on the assessment by MFI using the S2 and N antigens, only 3 serum samples showed double-negative results, indicating a false-negative rate of 1.3%. In 126 uninfected mouse sera, including 34 ELISA false-positive sera, only 7 samples showed false-positive results by MFI using either the S2 or N antigen (94% specificity). Similarly, the S2 and N antigen-based MFI was 98% sensitive and 100% specific in detecting anticoronavirus antibodies in rat sera. Thus, this MFI-based serologic assay using the S2 and N antigens promises to be a reliable diagnostic method, representing a highly sensitive and specific alternative to traditional ELISA for detection of coronavirus infections in laboratory mouse and rat colonies.

Sign in / Sign up

Export Citation Format

Share Document