scholarly journals Socialization of Providencia stuartii enables resistance to environmental insults.

2021 ◽  
Author(s):  
Julie Lopes ◽  
Guillaume TETREAU ◽  
Kevin Pounot ◽  
Mariam El Khatib ◽  
Jacques-Philippe Colletier
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Environmental Cues ◽  
Canonical Surface ◽  
High Concentrations ◽  
Ammonia Bicarbonate ◽  
Providencia Stuartii ◽  
Tract Infections

Providencia stuartii is a highly-social pathogen responsible for nosocomial chronic urinary tract infections. The bacterium indeed forms floating communities of cells (FCC) besides and prior-to canonical surface-attached biofilms (SAB). Within P. stuartii FCC, cells are riveted one to another owing to by self-interactions between its porins, viz. Omp-Pst1 and Omp-Pst2. In pathophysiological conditions, P. stuartii is principally exposed to high concentrations of urea, ammonia, bicarbonate, creatinine and to large variations of pH, questioning how these environmental cues affect socialization, and whether formation of SAB and FCC protects cells against those. Results from our investigations indicate that FCC and SAB can both form in the urinary tract, endowing cells with increased resistance and fitness. They additionally show that while Omp-Pst1 is the main gateway allowing penetration of urea, bicarbonate and ammonia into the periplasm, expression of Omp-Pst2 enables resistance to them.

scholarly journals Targeting Biofilm of MDR Providencia stuartii by Phages Using a Catheter Model

Antibiotics ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 375
Author(s):  
Chani Rakov ◽  
Shira Ben Porat ◽  
Sivan Alkalay-Oren ◽  
Ortal Yerushalmy ◽  
Mohanad Abdalrhman ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Phage Therapy ◽  
Multiple Drug ◽  
Bacterial Strains ◽  
Antibiotic Resistant ◽  
Isolation And Characterization ◽  
Wide Range ◽  
Providencia Stuartii ◽  
Tract Infections

Providencia spp. are emerging pathogens mainly in nosocomial infections. Providencia stuartii in particular is involved in urinary tract infections and contributes significantly to the high incidence of biofilm-formation in catheterized patients. Furthermore, recent reports suggested a role for multiple drug resistant (MDR) P. stuartii in hospital-associated outbreaks which leads to excessive complications resulting in challenging treatments. Phage therapy is currently one of the most promising solutions to combat antibiotic-resistant infections. However, the number of available phages targeting Providencia spp. is extremely limited, restricting the use of phage therapy in such cases. In the present study, we describe the isolation and characterization of 17 lytic and temperate bacteriophages targeting clinical isolates of Providencia spp. as part of the Israeli Phage Bank (IPB). These phages, isolated from sewage samples, were evaluated for host range activity and effectively eradicated 95% of the tested bacterial strains isolated from different geographic locations and displaying a wide range of antibiotic resistance. Their lytic activity is demonstrated on agar plates, planktonic cultures, and biofilm formed in a catheter model. The results suggest that these bacteriophages can potentially be used for treatment of antibiotic-resistant Providencia spp. infections in general and of urinary tract infections in particular.

scholarly journals Urinary Tract Infections Associated with Nontyphoidal Salmonella Serogroups

1999 ◽  
Vol 37 (12) ◽  
pp. 4177-4178 ◽  
Author(s):  
Sharon L. Abbott ◽  
Barbara A. Portoni ◽  
J. Michael Janda
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Pure Culture ◽  
Fecal Contamination ◽  
Microbial Diseases ◽  
High Concentrations ◽  
Tract Infections

In an analysis of over 23,000 nontyphoidal strains ofSalmonella submitted to the Microbial Diseases Laboratory between 1992 and 1996, two groups (C1 and E) were significantly recovered more often from the urinary tract than stool compared to more common groups such as B and D. An analysis of >60 urine isolates from 1996 suggests that most of these represent true urinary tract infections, as opposed to colonization or fecal contamination, by virtue of being isolated in pure culture and in high concentrations (>100,000 CFU/ml).

scholarly journals Nosocomial urinary tract infections caused by two O-serotypes of Providencia stuartii in one hospital

1977 ◽  
Vol 6 (6) ◽  
pp. 551-554
Author(s):  
G R Whiteley ◽  
J L Penner ◽  
I O Stewart ◽  
P C Stokan ◽  
N A Hinton
Keyword(s):  
Retrospective Study ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Epidemiological Studies ◽  
The Other ◽  
Positive Strain ◽  
Providencia Stuartii ◽  
Tract Infections

Providencia stuartii nosocomial urinary tract infections occurring in the same hospital over an 18-month period of retrospective study were shown, by serotyping and biotyping, to have been caused by two endemic strains. Two episodes, involving 38 patients in one ward and 11 patients in another, were caused by a mannitol-positive strain of serotype O55. Transmission of the strain through the movements of one patient appeared to have been the basis for the introduction of the agent from one ward to the other. In another episode, involving two patients in a third ward, the infections were caused by a mannitol-negative strain of serotype O49. The study demonstrated the usefulness of serotyping and biotyping in epidemiological studies of infections caused by P. stuartii.

Three Episodes of Nosocomial Urinary Tract Infections Caused by One O-serotype of Providencia Stuartii

1981 ◽  
Vol 125 (5) ◽  
pp. 668-671 ◽  
Author(s):  
John L. Penner ◽  
N.A. Hinton ◽  
Lesley J. Hamilton ◽  
Joan N. Hennessy
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Providencia Stuartii ◽  
Tract Infections

scholarly journals Targeting Biofilm of MDR Providencia stuartii by Phages Using a Catheter Model

2021 ◽  
Author(s):  
Chani Rakov ◽  
Shira Ben Porat ◽  
Sivan Alkalay-Oren ◽  
Ortal Yerushalmy ◽  
Mohanad Abdalrhman ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Phage Therapy ◽  
Multiple Drug ◽  
Bacterial Strains ◽  
Antibiotic Resistant ◽  
Isolation And Characterization ◽  
Wide Range ◽  
Providencia Stuartii ◽  
Tract Infections

AbstractProvidencia spp. are emerging pathogens mainly in nosocomial infections. Providencia stuartii in particular is involved in urinary tract infections and contributes significantly to the high incidence of biofilm-formation in catheterized patients. Furthermore, recent reports suggested a role for multiple drug resistant (MDR) P. stuartii in hospital-associated outbreaks which leads to excessive complications resulting in challenging treatments. Phage therapy is currently one of the most promising solutions to combat antibiotic-resistant infections. However, the number of available phages targeting Providencia spp. is extremely limited, restricting the use of phage therapy in such cases. In the present study, we describe the isolation and characterization of 17 lytic and lysogenic bacteriophages targeting clinical isolates of Providencia spp. as part of the Israeli Phage Bank (IPB). These phages, isolated from sewage samples, were evaluated for host range activity and effectively eradicated 95% of the tested bacterial strains isolated from different geographic locations and displaying a wide range of antibiotic resistance. Their lytic activity is demonstrated on agar plates, planktonic cultures, and biofilm formed in a catheter model. The results suggest that these bacteriophages can potentially be used for treatment of antibiotic-resistant Providencia spp. infections in general and of urinary tract infections in particular.

Susceptibility of Nosocomial Urinary Tract Infections Caused by Multiply Resistant Gram-Negative Bacilli Potential Use of Antimicrobials “Resistant” by Disc-Diffusion Testing for Controlling Epidemics

1980 ◽  
Vol 1 (3) ◽  
pp. 157-164 ◽  
Author(s):  
Walter E. Stamm ◽  
Joseph Arbaczawski ◽  
Donald C. Mackel ◽  
Roger L. Anderson
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Sensitivity Testing ◽  
Simultaneous Treatment ◽  
Gram Negative ◽  
Antimicrobial Sensitivity ◽  
Drug Concentrations ◽  
Mueller Hinton ◽  
High Concentrations ◽  
Tract Infections

AbstractProtracted hospital-based epidemics of urinary tract infection and bacteremia due to multiply resistant gram-negative bacilli have become an increasingly common and serious problem. Failure to control such outbreaks stems partly from inability to eradicate a key reservoir, the catheterized bladder. Since eradication of bacteriuria in noncatheterized patients can be achieved with single doses of antimicrobials and correlates with urinary rather than with serum antibiotic concentrations, drugs to which an organism appears resistant by discdiffusion testing, if excreted in the urine in high concentrations, might also prove useful in eliminating catheter-associated bacteriuria. Alternatively, urinary antiseptics, for which antimicrobial sensitivity testing is not usually done, might be effective. To test this hypothesis we determined the minimum inhibitory concentrations (MICs) of 45 multiply resistant Proteus, Serratia, Klebsiella, and Pseudomonas strains isolated in 13 recent epidemics of nosocomial urinary tract infections against 10 selected antimicrobials and urinary antiseptics, and compared these MICs with expected urinary concentrations of each drug. For each genus tested, MICs for at least two antimicrobials or urinary antiseptics were well below easily achievable urinary drug concentrations. Zone size criteria often predicted which drugs had MICs below achievable urinary levels. Little difference was found between MICs determined in Mueller-Hinton broth and in urine. During an epidemic, simultaneous treatment of all patients with bacteriuria by administration of a urinary antiseptic or an antibiotic that achieves high concentrations in urine, in conjunction with brief catheter removal, might prove useful in controlling any further infection.

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