scholarly journals Quantitative Analysis on the role ofRaffinose Synthasein Hippocampal Neurons

2017 ◽  
Author(s):  
Shatrunjai P. Singh ◽  
Vijendra P. Singh
Keyword(s):  
Oxidative Stress ◽  
Cell Lines ◽  
Hippocampal Neurons ◽  
Neuronal Cell ◽  
Biologically Active ◽  
Raffinose Synthase ◽  
Neuronal Cell Lines ◽  
Endogenous Antioxidant ◽  
Hpv 1 ◽  
Human And Mouse

1AbstractA diminished level of endogenous antioxidant in cells/tissues is associated with reduced resistance to oxidative stress. Raffinose synthase (RFS), a protective molecule regulates gene expression/function by controlling reactive oxygen species (ROS) levels, which has shown to be involved in a number of degenerative diseases. We confirmed the ubiquitous expression of this antioxidant protein in both human hippocampal neuron (HNN) and mouse hippocampal (HHPC-43) cell lines by an immunoblot and reverse transcriptase PCR (RT-PCR). Using a construct of RFS protein linked to CXCR-4, the transduction domain from HPV-1 CXCR-4 protein, we showed that RFS was transduced into both HNN, as well as HHPC-43 by the means of a western blot analysis. Further we proved that the protein was biologically active, and was shown to actively reduce the oxidative stress produced byparaquatand serum depletion in both human and mouse neuronal cell lines, increasing the viability of the cells. The results suggest that the intracellular delivery of RFS using CXCR-4 can be used to lower increased levels of ROS inside the cells and hence can be further investigated as a therapeutic tool in various ROS related neurodegenerative disorders.

scholarly journals Mutation of ATF4 mediates resistance of neuronal cell lines against oxidative stress by inducing xCT expression

2011 ◽  
Vol 19 (5) ◽  
pp. 847-858 ◽  
Author(s):  
J Lewerenz ◽  
H Sato ◽  
P Albrecht ◽  
N Henke ◽  
R Noack ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Lines ◽  
Neuronal Cell ◽  
Neuronal Cell Lines

scholarly journals Impact of Volatile Anesthetics on Oxidative Stress and Inflammation

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Yoon-Mi Lee ◽  
Byeng Chun Song ◽  
Kyung-Jin Yeum
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Cell Lines ◽  
Animal Studies ◽  
Neuronal Cell ◽  
Volatile Anesthetics ◽  
Human Studies ◽  
Rodent Models ◽  
Minor Surgery ◽  
Neuronal Cell Lines

The safety of anesthesia, which is an important step for surgery, can be determined by its impact on oxidative stress and inflammation. The effects of volatile anesthetics such as isoflurane and sevoflurane on oxidative stress and inflammation are reviewed in various (1) cell lines, (2) rodents, and (3) human studies. Isoflurane and sevoflurane are reported to have antioxidant and anti-inflammatory effects in all cells with exception of neuronal cell lines. In addition, various animal studies have indicated that isoflurane and sevoflurane were not only safe but also reduced oxidative stress and inflammation in rodent models. In human studies, oxidative stress, inflammation, and DNA damage were not affected by isoflurane and sevoflurane in patients undergoing minor incision surgeries. On the other hand, elevated oxidative stress, inflammation, and DNA damage have been observed in patients undergoing major surgeries such as abdominal and orthopedic surgeries, hysterectomy, cholecystectomy, and thoracotomy. Although impact of anesthetics on oxidative stress and inflammation is still not clear due to the variations of patients’ health conditions, types of surgery and the quantities of anesthetics, isoflurane, and sevoflurane can be considered safe anesthetics with respect to their effect on oxidative stress and inflammation in subjects undergoing minor surgery. Continuous effort evaluating the safety of anesthesia in various aspects is required.

On the Application of Cultured Neuronal Cell Lines in Neurotoxicological Studies: Cytotoxicity of Acrylamide

1983 ◽  
Vol 11 (3) ◽  
pp. 135-145
Author(s):  
Erik Walum
Keyword(s):  
Cell Lines ◽  
Culture Medium ◽  
Neuroblastoma Cells ◽  
Neuronal Cell ◽  
Leucine Incorporation ◽  
Biochemical Process ◽  
Neurite Retraction ◽  
Mouse Neuroblastoma ◽  
Neuronal Cell Lines

Summary Acrylamide, a well known neurotoxic compound, was used in a first evaluation of cultured mouse neuroblastoma cells as an alternative to animal models for neurotoxicological studies. Hence, the effects of acrylamide on the growth, size, morphology and leucine incorporation of three neuroblastoma (41A3, N18 and N1E115), one neuroblastoma x glioma hybrid (NG108CC15), two glioma (138MG and C6) and two fibroblast (RLF and RMC) cell lines were studied. It was found that the concentration of acrylamide needed to inhibit the growth by 50% in 24 hr was similar in all cell lines, i.e. around 2 x 10-4g/ml culture medium. In the two cell lines, N1E115 and NG108CC15, acrylamide at this concentration caused neurite retraction and at higher concentrations (5 x 10-4g/ml) a decrease in cell viability. In a concentration range of 5 x 10-5 - 5 x 10-4g/ml acrylamide did not affect cell size, or at 2 x 10-4g/ml incorporation of leucine into trichloroacetic acid precipitable material. It is suggested that acrylamide interferes with a biochemical process common to all the tested cells, but of greater importance in differentiated nerve cells than in others. Whether this process is consistent with the in vivo target for the neurotoxic action of acrylamide remains to be unravelled.

Differentiated Neuronal Cell Lines as Donor Tissue for Transplantation into the CNS

1987 ◽  
Vol 495 (1 Cell and Tiss) ◽  
pp. 767-770 ◽  
Author(s):  
MARY F. D. NOTTER ◽  
JEFFREY H. KORDOWER ◽  
DON M. GASH
Keyword(s):  
Cell Lines ◽  
Neuronal Cell ◽  
Donor Tissue ◽  
Neuronal Cell Lines
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