scholarly journals Cold trapped - Correcting locomotion dependent observation biases in thermal preference of Drosophila

2018 ◽  
Author(s):  
Diego Giraldo ◽  
Andrea K. Adden ◽  
Ilyas Kuhlemann ◽  
Heribert Gras ◽  
Bart R. H. Geurten
Keyword(s):  
Spatial Dimension ◽  
Temperature Gradients ◽  
Temperature Preference ◽  
Link Type ◽  
Online Simulation ◽  
Cold Area ◽  
Temperature Preferences ◽  
Group Aggregation ◽  
Environmental Temperatures ◽  
Dependent Observation

AbstractSensing environmental temperatures is essential for the survival of ectothermic organisms. In Drosophila, two methodologies are used to study temperature preferences (TP) and the genes involved in thermosensation: two-choice assays and temperature gradients. Whereas two-choice assays reveal a relative TP, temperature gradients can identify the absolute Tp. One drawback of gradients is that small ectothermic animals are susceptible to cold-trapping: a physiological inability to move at the cold area of the gradient. Often cold-trapping cannot be avoided, biasing the resulting TP to lower temperatures. Two mathematical models were previously developed to correct for cold-trapping. These models, however, focus on group behaviour which can lead to overestimation of cold-trapping due to group aggregation. Here we present a mathematical model that estimates the behaviour of individual Drosophilain temperature gradients. The model takes the spatial dimension and temperature difference of the gradient into account, as well as the rearing temperature of the flies. Furthermore, it allows quantifying cold-trapping, reveals true TP, and differentiates between temperature preference and tolerance. Online simulation is hosted at http://igloo.uni-goettingen.de. The code can be accessed at https://github.com/zerotonin/igloo.

Effects of Age and Overhead Illumination on Temperatures Preferred by Underyearling Rainbow Trout, Salmo gairdneri, in a Vertical Temperature Gradient

1978 ◽  
Vol 35 (11) ◽  
pp. 1430-1433 ◽  
Author(s):  
Wen-Hwa Kwain ◽  
Robert W. McCauley
Keyword(s):  
Rainbow Trout ◽  
Temperature Gradient ◽  
Temperature Gradients ◽  
Salmo Gairdneri ◽  
Vertical Temperature Gradient ◽  
Temperature Preference ◽  
Vertical Temperature ◽  
Preferred Temperature ◽  
Distribution Of Fish ◽  
Vertical Gradients

During their first 12 mo of life rainbow trout, Salmo gairdneri, preferred progressively cooler temperatures as they grew older; 19 °C was selected during the 1st mo and the selected temperature declined by intervals of 0.5 °C for each of the following months up to the 3rd mo. Fish swam higher in temperature gradients exposed to overhead illumination than in those in total darkness. This trend was reversed during the following 9 mo. These findings demonstrate the important role that age plays in the temperature preference of this species and the influence that overhead light may have on the distribution of fish in vertical gradients. Key words: preferred temperature, age, Salmo gairdneri, light gradients

Laboratory Methods for Determining Temperature Preference

1977 ◽  
Vol 34 (5) ◽  
pp. 749-752 ◽  
Author(s):  
Robert W. McCauley
Keyword(s):  
Temperature Gradient ◽  
Social Interactions ◽  
Physiological State ◽  
Thermal Gradients ◽  
Temperature Preference ◽  
Laboratory Methods ◽  
Preferred Temperature ◽  
Laboratory Techniques ◽  
Different Types ◽  
Temperature Preferences

Different types of temperature-gradient devices used in the laboratory to determine temperature preferences of fish are classified and reviewed. The type of device used seems to have less effect on experimental results than do other variables such as age, size, season, physiological state, or social interactions. Key words: preferred temperature, thermal gradients, thermoregulation, behavioral, gradient devices, laboratory techniques

Effects of temperature acclimation and age on movement of Cryptolestes ferrugineus (Coleoptera: Laemophloeidae) adults in response to temperature gradients

2005 ◽  
Vol 137 (1) ◽  
pp. 71-82 ◽  
Author(s):  
Fuji Jian ◽  
Digvir S. Jayas ◽  
Noel D.G. White
Keyword(s):  
Temperature Acclimation ◽  
Temperature Gradients ◽  
Acclimation Temperature ◽  
Cryptolestes Ferrugineus ◽  
Insect Behaviour ◽  
Cold Area ◽  
Effects Of Temperature ◽  
Response To Temperature ◽  
Short Time ◽  
Mixed Age

AbstractAdult Cryptolestes ferrugineus (Stephens) were reared at 30 °C or acclimated to 15, 25, or 35 °C for different periods (2 days or 1, 2, or 5 weeks). Populations 1 day old, 1, 5, 10, or 20 weeks old, or of mixed age were held in 10 cm × 10 cm × 100 cm wheat columns (14.5% ± 0.2% moisture content) with a temperature gradient (10 °C/m, 25–35 °C) or a constant temperature (25 or 30 °C) for 1, 6, or 12 days; movement and distribution were then determined. Acclimation to 25 °C for a short time (less than 2 days) did not influence adults' response to temperature in the wheat column. Adults acclimated to 15 and 35 °C responded differently to temperature in the wheat column, preferring temperatures above 30 and 35 °C, respectively. Increasing the duration of the acclimation to 15 or 35 °C resulted in higher aggregation. Acclimation temperature might be more important than exposure time for influencing insect behaviour. During their movement in the wheat columns, adults were also acclimated to the tested temperatures, and they did not always stay in a warmer area if the "cold" area was warmer than 25 °C. Adults that were 1 day old had a lower response (i.e., less movement) to temperature gradients than the older insects. Among the older insects, the response to temperature gradients did not decrease with increasing age, even at 20 weeks.

scholarly journals Evaluating tank acclimation and trial length for dynamic shuttle box temperature preference assays in aquatic animals

2021 ◽  
Author(s):  
Adam Alexander Harman ◽  
Meghan Fuzzen ◽  
Lisa Stoa ◽  
Douglas Boreham ◽  
Richard Manzon ◽  
...  
Keyword(s):  
Experimental Design ◽  
Experimental Designs ◽  
Thermal Preference ◽  
Coregonus Clupeaformis ◽  
Temperature Preference ◽  
Evolutionary Physiology ◽  
Shuttle Box ◽  
Average Temperature ◽  
Trial Length ◽  
Temperature Preferences

Characterizing the thermal preference of fish is important in conservation, environmental and evolutionary physiology and can be determined using a shuttle box system. Initial tank acclimation and trial lengths are important considerations in experimental design, yet systematic studies of these factors are missing. Three different behavioral assay experimental designs were tested to determine the effect of tank acclimation and trial length (12:12, 0:12, 2:2; hours of tank acclimation: behavioral trial) on the temperature preference of juvenile lake whitefish (Coregonus clupeaformis), using a shuttle box. Average temperature preferences for the 12:12, 0:12, and 2:2 experimental designs were 16.10±1.07°C, 16.02±1.56°C, 16.12±1.59°C respectively, with no significant differences between experimental designs (p= 0.9337). Ultimately, length of acclimation time and trial length had no significant effect on thermal preference.

scholarly journals Evaluating tank acclimation and trial length for shuttle box temperature preference assays

2020 ◽  
Author(s):  
Adam Alexander Harman ◽  
Meghan Fuzzen ◽  
Lisa Stoa ◽  
Douglas Boreham ◽  
Richard Manzon ◽  
...  
Keyword(s):  
Growth Temperature ◽  
Optimal Growth ◽  
Experimental Designs ◽  
Temperature Preference ◽  
Optimal Growth Temperature ◽  
Shuttle Box ◽  
Average Temperature ◽  
Trial Length ◽  
Temperature Preferences ◽  
System Average

AbstractThermal preferenda are largely defined by optimal growth temperature for a species and describe the range of temperatures an organism will occupy when given a choice. Assays for thermal preferenda require at least 24 hours, which includes a long acclimation to the tank, limits throughput and thus impacts replication in the study. Three different behavioral assay experimental designs were tested to determine the effect of tank acclimation and trial length (12:12, 0:12, 2:2; hours of tank acclimation: behavioral trial) on the temperature preference of juvenile lake whitefish, using a shuttle box system. Average temperature preferences for the 12:12, 0:12, and 2:2 experimental designs were 16.10 ± 1.07 °C, 16.02 ± 1.56 °C, 16.12 ± 1.59°C respectively, with no significant differences between the experimental designs (p= 0.9337). Ultimately, length of acclimation time and trial length had no significant impact, suggesting that all designs were equally useful for studies of temperature preference.

scholarly journals Spatial (in)justice and place-based strategies in innovation ecosystems: the case of the Alexander Innovation Zone in Thessaloniki

2020 ◽  
Vol 49 (49) ◽  
pp. 81-92
Author(s):  
Lefteris Topaloglou
Keyword(s):  
Social Justice ◽  
Spatial Dimension ◽  
Regional Level ◽  
Innovation Ecosystem ◽  
Equitable Distribution ◽  
Spatial Justice ◽  
Innovation Ecosystems ◽  
Link Type ◽  
Research Hypothesis ◽  
Empirical Material

AbstractThe concept of spatial justice relates to the fair and equitable distribution in space of socially valued resources and opportunities. In other words, spatial justice is the spatial dimension of social justice, placing more emphasis on the geography of distribution. On this basis, this paper examines the innovation ecosystem of the Alexander Innovation Zone of Thessaloniki in Greece. What is attempted is to scrutinise, through the lens of spatial justice, this state's initiative to deal at the regional level with innovation. This paper investigates whether a focus on localities and decentralisation would be better able to deliver the demands of spatial justice. The hypothesis to be tested is that equity in socially valued resources and opportunities can be better achieved through place-based strategies. Based upon empirical material, within the framework of the RELOCAL project (H2020, www.relocal.eu), this contribution attempts to shed some light on the aforementioned research hypothesis.

scholarly journals Temperature preference does not evolve in sticklebacks despite multigenerational exposure to elevated temperatures

2019 ◽  
Author(s):  
Natalie Pilakouta ◽  
Shaun S. Killen ◽  
Bjarni K. Kristjánsson ◽  
Skúli Skúlason ◽  
Jan Lindström ◽  
...  
Keyword(s):  
Climate Change ◽  
Gasterosteus Aculeatus ◽  
Elevated Temperatures ◽  
Thermal Environment ◽  
Adaptation To Climate Change ◽  
Evolutionary Potential ◽  
Temperature Preference ◽  
Preferred Temperature ◽  
Geothermal Activity ◽  
Temperature Preferences

ABSTRACTGiven the threat of climate change to biodiversity, a growing number of studies are investigating the potential for organisms to adapt to rising temperatures through changes in their physiology, morphology, and behaviour. Earlier work has predicted that evolutionary adaptation to climate change will be accompanied by a shift in temperature preferences, but empirical evidence for this is limited. Here, we test whether exposure to a warm environment over multiple generations has led to the evolution of higher preferred temperatures in the wild. We have taken advantage of a ‘natural experiment’ in Iceland, where freshwater populations of threespine sticklebacks (Gasterosteus aculeatus) are found in waters warmed by geothermal activity year-round (warm habitats), adjacent to populations in ambient-temperature lakes (cold habitats). We hypothesised that sticklebacks from warm habitats would prefer higher water temperatures than those from cold habitats, but we found no support for this hypothesis. Fish from both warm and cold habitats had an average preferred temperature of 13°C, which is considerably lower than the temperatures experienced by fish in warm habitats for most of the year. Our results therefore challenge the assumption that temperature preferences in ectotherms will readily evolve as a response to climate change. Furthermore, the fact that warm-habitat fish are able to persist at relatively high temperatures despite a lower temperature preference suggests that preferred temperature may be a poor indicator of a population’s evolutionary potential and capacity to adapt to a novel thermal environment.

Temperature preferences of three sympatric sticklebacks (Gasterosteidae)

1987 ◽  
Vol 65 (6) ◽  
pp. 1573-1576 ◽  
Author(s):  
Stephanie Lachance ◽  
Pierre Magnan ◽  
Gerard J. FitzGerald
Keyword(s):  
Gasterosteus Aculeatus ◽  
Sympatric Species ◽  
Habitat Partitioning ◽  
Pungitius Pungitius ◽  
Temperature Preference ◽  
Lawrence Estuary ◽  
Preferred Temperatures ◽  
Temperature Preferences ◽  
St Lawrence Estuary ◽  
Reproductive Migration

We determined the temperature preferences of three sympatric species of sticklebacks in the laboratory to see if differences in preferences could explain variation in times of reproductive migration to and from salt marsh tide pools along the southern shore of the St. Lawrence estuary, Quebec. The preferred temperatures were 9–12 °C for Gasterosteus aculeatus (form trachurus) and 11–14 °C for G. wheatlandi, while Pungitius pungitius showed a bimodal preference of 9–10 °C and 15–16 °C. In the field, G. aculeatus and G. wheatlandi left the tide pools earlier in the season than P. pungitius. Our results suggest that temperature preference may play a role in habitat partitioning among these species, hence facilitating their coexistence at high densities in the pools.

SIG 15 Perspectives Vol. 20, No. 3, September 2015

2015 ◽  
Vol 20 (3) ◽  
Keyword(s):  
Learning Center ◽  
Link Type

Abstract Download the CE Questions PDF from the toolbar, above. Use the questions to guide your Perspectives reading. When you're ready, purchase the activity from the ASHA Store and follow the instructions to take the exam in ASHA's Learning Center. Available until August 13, 2018.

SIG 11 Perspectives Vol. 22, No. 2, July 2012

2012 ◽  
Vol 22 (2) ◽  
Author(s):  
Kathryn Taylor ◽  
Emily White ◽  
Rachael Kaplan ◽  
Colleen M. O'Rourke
Keyword(s):  
Link Type

Sorry, this activity is no longer available for CEUs. Visit the SIG 11 page on the ASHA Store to see available CE activities. Use the CE questions PDF here as study questions to guide your Perspectives reading.

Sign in / Sign up

Export Citation Format

Share Document