scholarly journals In silico prediction and characterisation of secondary metabolite clusters in the plant pathogenic fungus Verticillium dahliae

2018 ◽  
Author(s):  
Xiaoqian Shi-Kunne ◽  
Roger de Pedro Jové ◽  
Jasper R.L. Depotter ◽  
Malaika Ebert ◽  
Michael F. Seidl ◽  
...  
Keyword(s):  
Secondary Metabolite ◽  
Verticillium Dahliae ◽  
In Silico ◽  
Host Plants ◽  
Biological Activities ◽  
Pathogenic Fungus ◽  
Pathogenic Fungi ◽  
Plant Pathogenic Fungi ◽  
In Silico Prediction ◽  
Close Proximity

ABSTRACTFungi are renowned producers of natural compounds, also known as secondary metabolites (SMs) that display a wide array of biological activities. Typically, the genes that are involved in the biosynthesis of SMs are located in close proximity to each other in so-called secondary metabolite clusters (SMCs). Many plant-pathogenic fungi secrete SMs during infection in order to promote disease establishment, for instance as cytocoxic compounds. Verticillium dahliae is a notorious plant pathogen that can infect over 200 host plants worldwide. However, the SM repertoire of this vascular pathogen remains mostly uncharted. To unravel the SM potential of V. dahliae, we performed in silico predictions and in-depth analyses of its SM clusters (SMC). We identified 25 potential SMCs in the V. dahliae genome, including loci that can be implicated in DHN-melanin, ferricrocin, triacetyl fusarinine and fujikurin production.

scholarly journals In silico prediction and characterisation of secondary metabolite clusters in the plant pathogenic fungus Verticillium dahliae

2019 ◽  
Vol 366 (7) ◽  
Author(s):  
Xiaoqian Shi-Kunne ◽  
Roger de Pedro Jové ◽  
Jasper R L Depotter ◽  
Malaika K Ebert ◽  
Michael F Seidl ◽  
...  
Keyword(s):  
Secondary Metabolite ◽  
Verticillium Dahliae ◽  
In Silico ◽  
Pathogenic Fungus ◽  
In Silico Prediction ◽  
Plant Pathogenic Fungus

scholarly journals Secondary Metabolites of the Rice Blast Fungus Pyricularia oryzae: Biosynthesis and Biological Function

2020 ◽  
Vol 21 (22) ◽  
pp. 8698
Author(s):  
Takayuki Motoyama
Keyword(s):  
Secondary Metabolites ◽  
Secondary Metabolism ◽  
Rice Blast ◽  
Plant Pathogens ◽  
Pathogenic Fungus ◽  
Pathogenic Fungi ◽  
Pyricularia Oryzae ◽  
Blast Disease ◽  
Plant Pathogenic Fungi ◽  
Biosynthetic Genes

Plant pathogenic fungi produce a wide variety of secondary metabolites with unique and complex structures. However, most fungal secondary metabolism genes are poorly expressed under laboratory conditions. Moreover, the relationship between pathogenicity and secondary metabolites remains unclear. To activate silent gene clusters in fungi, successful approaches such as epigenetic control, promoter exchange, and heterologous expression have been reported. Pyricularia oryzae, a well-characterized plant pathogenic fungus, is the causal pathogen of rice blast disease. P. oryzae is also rich in secondary metabolism genes. However, biosynthetic genes for only four groups of secondary metabolites have been well characterized in this fungus. Biosynthetic genes for two of the four groups of secondary metabolites have been identified by activating secondary metabolism. This review focuses on the biosynthesis and roles of the four groups of secondary metabolites produced by P. oryzae. These secondary metabolites include melanin, a polyketide compound required for rice infection; pyriculols, phytotoxic polyketide compounds; nectriapyrones, antibacterial polyketide compounds produced mainly by symbiotic fungi including endophytes and plant pathogens; and tenuazonic acid, a well-known mycotoxin produced by various plant pathogenic fungi and biosynthesized by a unique NRPS-PKS enzyme.

scholarly journals Biological activities of extracts fromAspidosperma subincanumMart. and in silico prediction for inhibition of acetylcholinesterase

2018 ◽  
Vol 32 (10) ◽  
pp. 2021-2033 ◽  
Author(s):  
Marina Pereira Rocha ◽  
Priscilla Rodrigues Valadares Campana ◽  
Denise de Oliveira Scoaris ◽  
Vera Lúcia de Almeida ◽  
Júlio César Dias Lopes ◽  
...  
Keyword(s):  
In Silico ◽  
Biological Activities ◽  
In Silico Prediction

scholarly journals A Review on the Potency of Melaleuca leucadendron Leaves Solid Waste in Wood Preservation and Its In Silico Prediction upon Biological Activities

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Christine Patramurti ◽  
Radjali Amin ◽  
Christofori M. R. R. Nastiti ◽  
Maywan Hariono
Keyword(s):  
Chemical Composition ◽  
Essential Oils ◽  
Solid Waste ◽  
In Silico ◽  
Leaf Morphology ◽  
Biological Activities ◽  
Wood Preservative ◽  
Healthcare Management ◽  
Wood Preservation ◽  
In Silico Prediction

The essential oils from cajuput leaves (Melaleuca leucadendron) have been well-known and applied, especially in healthcare management. However, the utilization of the leaves solid waste has not been explored and reported in detail. In this review, we elaborate on the cajuput leaves starting from the plant description and leaf morphology, chemical composition, biological activities, wood decomposing organism, and an in silico prediction upon its molecular mechanism. Based on the in silico prediction, compounds such as guaiol, lupene, and 1, 8-cineole have the potential to be antifungal and insecticide that associates with the cajuput potency as a wood preservative agent.

scholarly journals Glycosylated Flavonoids from Psidium guineense as Major Inhibitors of HIV-1 Replication in vitro

2017 ◽  
Vol 12 (7) ◽  
pp. 1934578X1701200
Author(s):  
Joseph T Ortega ◽  
Omar Estrada ◽  
Maria L Serrano ◽  
Whendy Contreras ◽  
Giovannina Orsini ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Reverse Transcriptase ◽  
In Silico ◽  
Biological Activities ◽  
In Silico Prediction ◽  
Flavonoid Quercetin ◽  
Derivatives Of ◽  
Anti Hiv ◽  
Hiv 1

Flavonoids are present in practically all plants and many biological activities have been described for them. The flavonoid quercetin is a common molecule for which anti-HIV activity has been demonstrated. Avicularin and guajaverin are derivatives of quercetin with a glycoside substituent in their structure. In this work, a mixture of both derivatives was purified from an extract of Psidium guinense. The mixture exhibited activity against HIV-1 in vitro, with an IC50 of approximately 8.5 μg/mL, which compares favorably with the IC50 of 53 μg/mL of quercetin. The mixture also inhibited HIV-1 reverse transcriptase (RT), with an IC50 of 7.2 μM, compared to 0.6 μM for quercetin. These results are in agreement with the in silico prediction for the interaction of these flavonoids with RT and suggest that the glycosylic moiety could favor the transport of the compound into the cell. However, the glycosidic moiety might be cleaved intracellularly, being the resultant quercetin responsible for the antiviral activity.

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