Patch-Clamp and Perfusion Techniques to Study Ion Channels Expressed in Xenopus Oocytes

Novel α-aminoamide derivatives containing different benzoheterocyclics moiety were synthesized and evaluated as voltage-gated sodium ion channels blocks the treatment of pain. Compounds 6a, 6e, and 6f containing the benzofuran group displayed more potent in vivo analgesic activity than ralfinamide in both the formalin test and the writhing assay. Interestingly, they also exhibited potent in vitro anti-Nav1.7 and anti-Nav1.8 activity in the patch-clamp electrophysiology assay. Therefore, compounds 6a, 6e, and 6f, which have inhibitory potency for two pain-related Nav targets, could serve as new leads for the development of analgesic medicines.

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Is manual patch clamp still the gold standard assay for ion channels?

Journal of Pharmacological and Toxicological Methods ◽

10.1016/j.vascn.2016.02.016 ◽

2016 ◽

Vol 81 ◽

pp. 339

Author(s):

Elisa Ballini ◽

Corrado Carignani ◽

Ezio Bettini ◽

Maria Pilla

Keyword(s):

Ion Channels ◽

Patch Clamp ◽

Gold Standard ◽

Standard Assay

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Single-Channel and Whole-Cell Patch-Clamp Experiments on Gland Cells: Activation of Ion Channels Via Internal Messengers

Cell Membrane Transport ◽

10.1007/978-1-4757-9601-8_22 ◽

1991 ◽

pp. 437-450

Author(s):

Ole Petersen

Keyword(s):

Ion Channels ◽

Patch Clamp ◽

Single Channel ◽

Whole Cell ◽

Gland Cells ◽

Cell Patch Clamp ◽

Whole Cell Patch Clamp

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Ion channel activities of cultured rat mesangial cells

AJP Renal Physiology ◽

10.1152/ajprenal.1991.261.5.f808 ◽

1991 ◽

Vol 261 (5) ◽

pp. F808-F814 ◽

Cited By ~ 4

Author(s):

H. Matsunaga ◽

N. Yamashita ◽

Y. Miyajima ◽

T. Okuda ◽

H. Chang ◽

...

Keyword(s):

Angiotensin Ii ◽

Ion Channels ◽

Patch Clamp ◽

Ion Channel ◽

Arginine Vasopressin ◽

Mesangial Cells ◽

Cation Channel ◽

K Channel ◽

Patch Clamp Technique ◽

Inside Out

We used the patch-clamp technique to clarify the nature of ion channels in renal mesangial cells in culture. In the cell-attached mode most patches were silent in the absence of agonists. In some patches a 25-pS nonselective channel was observed. This 25-pS cation channel was consistently observed in inside-out patches, and it was activated by intracellular Ca2+. Excised patch experiments also revealed the existence of a 40-pS K+ channel, which was activated by intracellular Ca2+. This 40-pS K+ channel was observed infrequently in the cell-attached mode. The activities of both channels were increased by arginine vasopressin or angiotensin II, resulting from an increase in intracellular Ca2+ concentration.

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