Whole-Cell Recording and Voltage-Sensitive Dye Imaging In Vivo

2009 ◽  
Vol 2009 (6) ◽  
pp. pdb.prot5232-pdb.prot5232 ◽  
Author(s):  
C. Petersen
Keyword(s):  
Voltage Sensitive Dye ◽  
Whole Cell ◽  
Whole Cell Recording ◽  
Cell Recording ◽  
Voltage Sensitive Dye Imaging

scholarly journals Compensation of physiological motion enables high-yield whole-cell recording in vivo

2020 ◽  
Author(s):  
William M. Stoy ◽  
Bo Yang ◽  
Ali Kight ◽  
Nathaniel C. Wright ◽  
Peter Y. Borden ◽  
...  
Keyword(s):  
Single Cells ◽  
Strong Dependence ◽  
High Yield ◽  
Patch Clamp Recording ◽  
Gold Standard Method ◽  
Whole Cell ◽  
Whole Cell Recording ◽  
Cell Recording ◽  
Living Mouse

1.1.1AbstractWhole-cell patch-clamp recording in vivo is the gold-standard method for measuring subthreshold electrophysiology from single cells during behavioural tasks, sensory stimulations, and optogenetic manipulation. However, these recordings require a tight, gigaohm resistance, seal between a glass pipette electrode’s aperture and a cell’s membrane. These seals are difficult to form, especially in vivo, in part because of a strong dependence on the distance between the pipette aperture and cell membrane. We elucidate and utilize this dependency to develop an autonomous method for placement and synchronization of pipette’s tip aperture to the membrane of a nearby, moving neuron, which enables high-yield seal formation and subsequent recordings in the deep in the brain of the living mouse, in the thalamus. This synchronization procedure nearly doubles the reported gigaseal yield in the thalamus (>3 mm below the pial surface) from 26% (n=17/64) to 48% (n=32/66). Whole-cell recording yield improved from 10% (n = 9/88) to 24% (n=18/76) when motion compensation was used during the gigaseal formation. As an example of its application, we utilized this system to investigate the role of the sensory environment and ventral posterior medial region (VPM) projection synchrony on intracellular dynamics in the barrel cortex. This method results in substantially greater subcortical whole-cell recording yield than previously reported and thus makes pan-brain whole-cell electrophysiology practical in the living mouse brain.

In vivo whole-cell recording from neurons of the superior colliculus in fetal rats

1998 ◽  
Vol 108 (1-2) ◽  
pp. 255-262 ◽  
Author(s):  
Yoshiyuki Sakata ◽  
Takashi Fujioka ◽  
Shoji Nakamura
Keyword(s):  
Superior Colliculus ◽  
Whole Cell ◽  
Whole Cell Recording ◽  
Fetal Rats ◽  
Cell Recording

scholarly journals In Vivo Whole-Cell Recording Analysis of Nociceptive Transmission in the Rat Spinal Dorsal Horn

PAIN RESEARCH ◽  
1999 ◽  
Vol 14 (2) ◽  
pp. 73-79 ◽  
Author(s):  
Hidemasa Furue ◽  
Keita Narikawa ◽  
Eiichi Kumamoto ◽  
Megumu Yoshimura
Keyword(s):  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Whole Cell ◽  
Nociceptive Transmission ◽  
Spinal Dorsal ◽  
Whole Cell Recording ◽  
Cell Recording ◽  
Recording Analysis

scholarly journals In Vivo Whole-Cell Recording Analysis of Inhibitory Synaptic Responses in Substantia Gelatinosa Neurons of the Rat Spinal Cord

PAIN RESEARCH ◽  
2000 ◽  
Vol 15 (2) ◽  
pp. 75-80
Author(s):  
Keita Narikawa ◽  
Hidemasa Furue ◽  
Eiichi Kumamoto ◽  
Megumu Yoshimura
Keyword(s):  
Spinal Cord ◽  
Substantia Gelatinosa ◽  
Rat Spinal Cord ◽  
Whole Cell ◽  
Whole Cell Recording ◽  
Cell Recording ◽  
Recording Analysis ◽  
Synaptic Responses

scholarly journals High-throughput cellular-resolution synaptic connectivity mapping in vivo with concurrent two-photon optogenetics and volumetric Ca2+ imaging

2020 ◽  
Author(s):  
Christopher McRaven ◽  
Dimitrii Tanese ◽  
Lixia Zhang ◽  
Chao-Tsung Yang ◽  
Misha B. Ahrens ◽  
...  
Keyword(s):  
High Throughput ◽  
Synaptic Connectivity ◽  
Whole Cell ◽  
Two Photon ◽  
Whole Cell Recording ◽  
Connectivity Mapping ◽  
Cellular Resolution ◽  
Cell Recording ◽  
Undulatory Locomotion

AbstractThe ability to measure synaptic connectivity and properties is essential for understanding neuronal circuits. However, existing methods that allow such measurements at cellular resolution are laborious and technically demanding. Here, we describe a system that allows such measurements in a high-throughput way by combining two-photon optogenetics and volumetric Ca2+ imaging with whole-cell recording. We reveal a circuit motif for generating fast undulatory locomotion in zebrafish.

Rise time analysis of visually evoked synaptic potentials in tectal neurons of the frog: In vivo perforated whole cell recording

1994 ◽  
Vol 19 ◽  
pp. S206
Author(s):  
Eriko Sadamori ◽  
Hideki Nakagawa ◽  
Nobuyoshi Matsumoto
Keyword(s):  
Rise Time ◽  
Time Analysis ◽  
Whole Cell ◽  
Synaptic Potentials ◽  
Whole Cell Recording ◽  
Cell Recording ◽  
Tectal Neurons

scholarly journals Compensation of physiological motion enables high-yield whole-cell recording in vivo

2021 ◽  
Vol 348 ◽  
pp. 109008
Author(s):  
William M. Stoy ◽  
Bo Yang ◽  
Ali Kight ◽  
Nathaniel C. Wright ◽  
Peter Y. Borden ◽  
...  
Keyword(s):  
High Yield ◽  
Whole Cell ◽  
Whole Cell Recording ◽  
Cell Recording ◽  
Physiological Motion
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