scholarly journals Direct Observation of Large Temperature Fluctuations during DNA Thermal Denaturation

2006 ◽  
Vol 96 (3) ◽  
Author(s):  
K. S. Nagapriya ◽  
A. K. Raychaudhuri ◽  
Dipankar Chatterji
2017 ◽  
Vol 56 (4) ◽  
pp. 1083-1098 ◽  
Author(s):  
Matthew E. Jeglum ◽  
Sebastian W. Hoch ◽  
Derek D. Jensen ◽  
Reneta Dimitrova ◽  
Zachariah Silver

AbstractLarge temperature fluctuations (LTFs), defined as a drop of the near-surface temperature of at least 3°C in less than 30 min followed by a recovery of at least half of the initial drop, were frequently observed during the Mountain Terrain Atmospheric Modeling and Observations (MATERHORN) program. Temperature time series at over 100 surface stations were examined in an automated fashion to identify and characterize LTFs. LTFs occur almost exclusively at night and at locations elevated 50–100 m above the basin floors, such as the east slope of the isolated Granite Mountain (GM). Temperature drops associated with LTFs were as large as 13°C and were typically greatest at heights of 4–10 m AGL. Observations and numerical simulations suggest that LTFs are the result of complex flow interactions of stably stratified flow with a mountain barrier and a leeside cold-air pool (CAP). An orographic wake forms over GM when stably stratified southwesterly nocturnal flow impinges on GM and is blocked at low levels. Warm crest-level air descends in the lee of the barrier, and the generation of baroclinic vorticity leads to periodic development of a vertically oriented vortex. Changes in the strength or location of the wake and vortex cause a displacement of the horizontal temperature gradient along the slope associated with the CAP edge, resulting in LTFs. This mechanism explains the low frequency of LTFs on the west slope of GM as well as the preference for LTFs to occur at higher elevations later at night, as the CAP depth increases.


Author(s):  
Qingjun Cai ◽  
Chung-Lung Chen ◽  
Julie F. Asfia

Pulsating heat pipe (PHP) is an interesting heat transfer device. This paper focuses on PHP heat transfer characteristics versus its operating temperature. In experiments of copper and stainless steel PHPs, results indicate that, at a same input power, both temperature differences (from the evaporator to condenser) and evaporator temperature fluctuations are relevant to operating temperature. The minimal temperature difference and fluctuation appear at an operating temperature ranging from 120 to 160°C. Experimental analysis of the evaporator temperature fluctuations show that single phase cooling in PHP causes a large temperature difference (between the evaporator and condenser) and fluctuation. Phase change proportion increases at the optimal operation condition. Static mechanical balance combining with dynamic elastic oscillation system is used to interpret PHP temperature variations versus operating temperature.


2010 ◽  
Vol 99 (8) ◽  
pp. 2666-2675 ◽  
Author(s):  
K.S. Nagapriya ◽  
A.K. Raychaudhuri

2018 ◽  
Author(s):  
LUO Liaofu ◽  
YANG Guochen

AbstractA quantum model on DNA structure is proposed. By introducing the self-consistent harmonic potential in the X-Y plane vertical to helix axis (z-direction) and the periodic potential along the z-axis we obtain the wave function for the single nucleotide and the many-nucleotide system. The helix distribution of bases is deduced from the solution of wave function under the self-consistent potential. The variation of DNA structure (polymorphism) is related to the periodicity of the potential in Z-axis, the quantum state occurring in harmonic potential and the interaction between helix strands. As Watson-Crick (W-C) interaction is introduced between double helices, the quasi-particle transformation is utilized to solve the interacting many-body problem for DNA. It is proved that the phase-transition (thermal denaturation) temperature is related to the frequency ω of harmonic potential. Through comparison with experimental data a simple relation (N means number of base pairs and V the W-C coupling) is deduced. For a DNA sequence of 1000 bp ω is predicted about (0.9-1.2)×1017/sec. Such a high frequency is necessary for nucleotides of each strand located on a narrow tube. The large temperature fluctuation experimentally observed during DNA thermal denaturation is interpreted by the collective motion of nucleotides.


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