scholarly journals Protein and Nucleic Acid Metabolism in Fruits: I. Studies of Amino Acid Incorporation During the Climacteric Rise in Respiration of the Avocado

1966 ◽  
Vol 41 (8) ◽  
pp. 1247-1253 ◽  
Author(s):  
Amos Richmond ◽  
Jacob B. Biale
2021 ◽  
Vol 8 ◽  
Author(s):  
Ce Shi ◽  
Yangfang Ye ◽  
Feng Pei ◽  
Changkao Mu ◽  
Chunlin Wang

Demand from consumers for small quantities of live swimming crab Portunus trituberculatus is rising with the development of e-commerce. However, it is challenging to keep P. trituberculatus alive post-capture. In this study, a transport bag containing oxygen and seawater (24 ppt) was used to investigate the survival and metabolic changes of P. trituberculatus during transport. The results showed that more than 80% P. trituberculatus could survive at least 24 h in the transport bag. The ability of the crabs to survive may be attributed to adaptive metabolism, as suggested by the switch from an aerobic to an anaerobic pathway for energy generation and a decline in amino acid metabolism, nucleic acid metabolism, and osmoregulation. Our findings suggest that the transport bag could effectively extend the post-capture survival time of P. trituberculatus. Metabolic adaptation – especially energy homeostasis – is crucial for crab survival during transport. Our study provides a promising method for the transport of live P. trituberculatus.


1963 ◽  
Vol 9 (3) ◽  
pp. 337-341 ◽  
Author(s):  
MICHIO OISHI ◽  
SHIGERU KITAYAMA ◽  
HAJIME TAKAHASHI ◽  
BUNJI MARUO

Amino Acids ◽  
2020 ◽  
Author(s):  
Thomas L. Williams ◽  
Debra J. Iskandar ◽  
Alexander R. Nödling ◽  
Yurong Tan ◽  
Louis Y. P. Luk ◽  
...  

AbstractGenetic code expansion is a powerful technique for site-specific incorporation of an unnatural amino acid into a protein of interest. This technique relies on an orthogonal aminoacyl-tRNA synthetase/tRNA pair and has enabled incorporation of over 100 different unnatural amino acids into ribosomally synthesized proteins in cells. Pyrrolysyl-tRNA synthetase (PylRS) and its cognate tRNA from Methanosarcina species are arguably the most widely used orthogonal pair. Here, we investigated whether beneficial effect in unnatural amino acid incorporation caused by N-terminal mutations in PylRS of one species is transferable to PylRS of another species. It was shown that conserved mutations on the N-terminal domain of MmPylRS improved the unnatural amino acid incorporation efficiency up to five folds. As MbPylRS shares high sequence identity to MmPylRS, and the two homologs are often used interchangeably, we examined incorporation of five unnatural amino acids by four MbPylRS variants at two temperatures. Our results indicate that the beneficial N-terminal mutations in MmPylRS did not improve unnatural amino acid incorporation efficiency by MbPylRS. Knowledge from this work contributes to our understanding of PylRS homologs which are needed to improve the technique of genetic code expansion in the future.


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