scholarly journals The Relationship between H2 Evolution and Acetylene Reduction in Pisum sativum-Rhizobium leguminosarum Symbioses Differing in Uptake Hydrogenase Activity

1986 ◽  
Vol 82 (1) ◽  
pp. 154-159 ◽  
Author(s):  
John D. Mahon ◽  
Louise M. Nelson
Keyword(s):  
Pisum Sativum ◽  
Acetylene Reduction ◽  
Rhizobium Leguminosarum ◽  
Hydrogenase Activity ◽  
Uptake Hydrogenase ◽  
H2 Evolution ◽  
The Relationship

Acetylene reduction and hydrogen evolution by nitrogenase in a Rhizobium–legume symbiosis

1983 ◽  
Vol 61 (3) ◽  
pp. 780-785 ◽  
Author(s):  
Scott A. Edie
Keyword(s):  
Hydrogen Evolution ◽  
Acetylene Reduction ◽  
Rhizobium Leguminosarum ◽  
High Light ◽  
Hydrogenase Activity ◽  
Uptake Hydrogenase ◽  
Vegetative Phase ◽  
Pisum Sativum L ◽  
Legume Symbiosis ◽  
The Relationship

The relationship between acetylene reduction and hydrogen evolution in air was examined in peas (Pisum sativum L. cv. Alaska) inoculated with Rhizobium leguminosarum strain 3740, which lacked uptake hydrogenase activity (Hup−). In the absence of a system for recycling hydrogen, changes in the relative efficiency of N2 fixation (RE), which is defined as RE = 1 − (hydrogen evolved in air)/(acetylene reduced), presumably reflect an altered capacity of nitrogenase to allocate electrons between protons and N2. The RE of plants grown without combined nitrogen declined during the vegetative phase of growth and increased after flowering. Continuous high light or elevated CO2 conditions in the absence of nitrate maintained throughout ontogeny accentuated decreases in RE. When nitrate was present in the growth medium declines in RE during the vegetative phase were lessened. These results are consistent with the concept that the electron allocation coefficient of nitrogenase varies in the absence of uptake hydrogenase activity.

Pisum sativum cultivar effects on hydrogen metabolism in Rhizobium

1984 ◽  
Vol 62 (8) ◽  
pp. 1682-1686 ◽  
Author(s):  
Eulogio J. Bedmar ◽  
Donald A. Phillips
Keyword(s):  
Pisum Sativum ◽  
Relative Efficiency ◽  
Rhizobium Leguminosarum ◽  
Root Nodules ◽  
Developmental Differences ◽  
The Other ◽  
Hydrogenase Activity ◽  
Uptake Hydrogenase ◽  
Hydrogen Metabolism ◽  
Pisum Sativum L

Data from 14 Pisum sativum L. cultivars establish that three pea genotypes, which were previously reported to affect net H2 evolution from root nodules in air and uptake hydrogenase activity of Rhizobium leguminosarum 128C53, are not unique. Two pea lines, 'JI1205' and 'Green Arrow,' produced very active uptake hydrogenase activity in strain 128C53, and essentially no H2 was evolved in air from root nodules capable of reducing 20 μmol C2H2 ∙ plan−1 ∙ h−1. Five other cultivars produced significantly lower uptake hydrogenase activities in the same bacterial strain and had much higher rates of net H2 evolution with similar C2H2-reduction capabilities. Parallel experiments with the same cultivars nodulated by R. leguminosarum 300, an organism with no convincing uptake hydrogenase activity in any pea line, showed that 'JI1205' and 'Green Arrow' had a significantly lower relative efficiency (RE) of N2 fixation (1 − (H2 evolved in air/C2H2 reduced)) than the other five cultivars. Developmental differences among the pea lines prevented any conclusion about the advantages or disadvantages of uptake hydrogenase activity for plant growth, but in general, cultivars with high uptake hydrogenase activity and low net H2 evolution grew more slowly than those evolving large amounts of H2.

scholarly journals Development and Partial Characterization of Nearly Isogenic Pea Lines (Pisum sativum L.) that Alter Uptake Hydrogenase Activity in Symbiotic Rhizobium

1990 ◽  
Vol 92 (4) ◽  
pp. 983-989 ◽  
Author(s):  
Donald A. Phillips ◽  
Yoram Kapulnik ◽  
Eulogio J. Bedmar ◽  
Cecillia M. Joseph
Keyword(s):  
Pisum Sativum ◽  
Partial Characterization ◽  
Hydrogenase Activity ◽  
Uptake Hydrogenase ◽  
Pisum Sativum L

scholarly journals Uptake Hydrogenase Activity Determined by Plasmid pRL6JI in Rhizobium leguminosarum Does Not Increase Symbiotic Nitrogen Fixation

1985 ◽  
Vol 50 (4) ◽  
pp. 791-794 ◽  
Author(s):  
Scott D. Cunningham ◽  
Yoram Kapulnik ◽  
Nicholas J. Brewin ◽  
Donald A. Phillips
Keyword(s):  
Nitrogen Fixation ◽  
Rhizobium Leguminosarum ◽  
Symbiotic Nitrogen Fixation ◽  
Hydrogenase Activity ◽  
Uptake Hydrogenase

scholarly journals Uptake hydrogenase activity and ATP formation in Rhizobium leguminosarum bacteroids.

1982 ◽  
Vol 151 (2) ◽  
pp. 989-995 ◽  
Author(s):  
L M Nelson ◽  
S O Salminen
Keyword(s):  
Rhizobium Leguminosarum ◽  
Hydrogenase Activity ◽  
Uptake Hydrogenase ◽  
Atp Formation

scholarly journals Rhizobium leguminosarum Biovar viciae Symbiotic Hydrogenase Activity and Processing Are Limited by the Level of Nickel in Agricultural Soils

2005 ◽  
Vol 71 (11) ◽  
pp. 7603-7606 ◽  
Author(s):  
Ana-Claudia Ureta ◽  
Juan Imperial ◽  
Tomás Ruiz-Argüeso ◽  
Jose M. Palacios
Keyword(s):  
Pisum Sativum ◽  
Rhizobium Leguminosarum ◽  
Agricultural Soils ◽  
Hydrogenase Activity ◽  
Inoculant Strain

ABSTRACT Analysis of levels of hydrogenase processing and activity in Rhizobium leguminosarum biovar viciae bacteroids from pea (Pisum sativum) plants showed that the oxidation of nitrogenase-evolved hydrogen is limited by the availability of nickel in agricultural soils. This limitation was overcome by using an inoculant strain engineered for higher hydrogenase expression.

Nitrogen fixation and hydrogen metabolism by Rhizobium leguminosarum isolates in pea root nodules

1981 ◽  
Vol 27 (10) ◽  
pp. 1028-1034 ◽  
Author(s):  
Louise M. Nelson ◽  
J. J. Child
Keyword(s):  
Relative Efficiency ◽  
Rhizobium Leguminosarum ◽  
High Efficiency ◽  
Root Nodules ◽  
Dry Weight ◽  
Hydrogenase Activity ◽  
Uptake Hydrogenase ◽  
Hydrogen Metabolism ◽  
N Content ◽  
Significant Uptake

A survey of 108 isolates of Rhizobium leguminosarum was conducted to determine the variation in H2 uptake and relative efficiency of N2 fixation in Pisum sativum L. root nodules and the relation of relative efficiency to plant dry weight and N content. Only 14 of the isolates exhibited significant uptake hydrogenase activity and none of these had sufficient hydrogenase activity to recycle all of the H2 produced by nitrogenase. In 74 of the isolates tested relative efficiencies of N2 fixation were less than 0.60.Twenty-nine of the isolates were ineffective, since total plant N at harvest did not differ significantly from uninoculated controls. The remaining 79 effective isolates could be divided into low-and high-efficiency groups. Plants which were inoculated with isolates from the two groups and harvested after 4 weeks did not differ significantly in plant dry weight or N content. Among the isolates with high relative efficiency of N2 fixation, two groups could be recognized: one possessing significant uptake hydrogenase activity, the other lacking hydrogenase activity but in which H2 evolution was low. Although the two groups did not differ with respect to plant dry weight or N content, the identification of this latter group may be of some significance for optimizing the efficiency of N2 fixation.

Interaction between hydrogenase, nitrogenase, and respiratory activities in a Frankia isolate from Alnus rubra

1989 ◽  
Vol 35 (6) ◽  
pp. 636-641 ◽  
Author(s):  
Marcia A. Murry ◽  
Mary F. Lopez
Keyword(s):  
Nitrogen Fixation ◽  
Acetylene Reduction ◽  
Root Nodules ◽  
Hydrogenase Activity ◽  
Alnus Rubra ◽  
Uptake Hydrogenase ◽  
Frankia Strain ◽  
Intact Cells

H2 uptake and H2-supported O2 uptake were measured in N2-fixing cultures of Frankia strain ArI3 isolated from root nodules of Alnus rubra. H2 uptake by intact cells was O2 dependent and maximum rates were observed at ambient O2 concentrations. No hydrogenase activity could be detected in [Formula: see text], undifferentiated filaments cultured aerobically indicating that uptake hydrogenase activity was associated with the vesicles, the cellular site of nitrogen fixation in Frankia. Hydrogenase activity was inhibited by acetylene but inhibition could be alleviated by pretreatment with H2. H2 stimulated acetylene reduction at supraoptimal but not suboptimal O2 concentrations. These results suggest that uptake hydrogenase activity in ArI3 may play a role in O2 protection of nitrogenase, especially under conditions of carbon limitation.Key words: Frankia, hydrogenase, nitrogenase, O2 protection.

scholarly journals NodC-based evaluation of the occurrence of bacteria in nodules of Pisum sativum isolated on YEM agar

2019 ◽  
Vol 70 (1) ◽  
pp. 59-67
Author(s):  
Anna Lenart-Boroń ◽  
Tadeusz Zając ◽  
Piotr Mateusz Boroń ◽  
Agnieszka Klimek-Kopyra
Keyword(s):  
Pisum Sativum ◽  
Rhizobium Leguminosarum ◽  
Reference Strain ◽  
Root Nodules ◽  
Bacterial Species ◽  
Soil Science ◽  
Associated Bacteria ◽  
Plant Cultivation ◽  
Yeast Extract Mannitol ◽  
Diverse Community

SummaryThe bacterial nodulation (nod) genes are essential in the formation process of root nodules. This study was aimed to verify the occurrence of nodule-associated bacteria in two pea varieties (“Tarchalska” and “Klif ”) inoculated withRhizobiuminoculants – Nitragine™ and a noncommercial one produced by the Polish Institute of Soil Science and Plant Cultivation (IUNG). The number of colonies isolated on yeast extract mannitol (YEM) agar from the nodules of “Klif ” inoculated with IUNG inoculants was significantly higher than the number of colonies isolated from other variants. Species identification was based on sequencing of 16S rDNA, which revealed that despite careful sterilization of nodules, sequences of other bacterial species were detected. Among them, one sequence belonged toRhizobium leguminosarum(isolated from IUNG inoculant). To assess the presence of nodulation-capableRhizobium, amplification of thenodCgene was performed, which revealed that of 29 samples, 19 were positive. The remaining isolates, including reference strain and bacteria isolated from Nitragine™, lacked this gene. The results show that pea nodules harbor a very diverse community of bacteria. The lack ofnodCgene in some strains isolated from plants inoculated with Nitragine™ and with IUNG inoculant proves that even ifR. leguminosarumare abundant, they may not be efficient in nodulation.

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