Pisum sativum cultivar effects on hydrogen metabolism in Rhizobium

1984 ◽  
Vol 62 (8) ◽  
pp. 1682-1686 ◽  
Author(s):  
Eulogio J. Bedmar ◽  
Donald A. Phillips
Keyword(s):  
Pisum Sativum ◽  
Relative Efficiency ◽  
Rhizobium Leguminosarum ◽  
Root Nodules ◽  
Developmental Differences ◽  
The Other ◽  
Hydrogenase Activity ◽  
Uptake Hydrogenase ◽  
Hydrogen Metabolism ◽  
Pisum Sativum L

Data from 14 Pisum sativum L. cultivars establish that three pea genotypes, which were previously reported to affect net H2 evolution from root nodules in air and uptake hydrogenase activity of Rhizobium leguminosarum 128C53, are not unique. Two pea lines, 'JI1205' and 'Green Arrow,' produced very active uptake hydrogenase activity in strain 128C53, and essentially no H2 was evolved in air from root nodules capable of reducing 20 μmol C2H2 ∙ plan−1 ∙ h−1. Five other cultivars produced significantly lower uptake hydrogenase activities in the same bacterial strain and had much higher rates of net H2 evolution with similar C2H2-reduction capabilities. Parallel experiments with the same cultivars nodulated by R. leguminosarum 300, an organism with no convincing uptake hydrogenase activity in any pea line, showed that 'JI1205' and 'Green Arrow' had a significantly lower relative efficiency (RE) of N2 fixation (1 − (H2 evolved in air/C2H2 reduced)) than the other five cultivars. Developmental differences among the pea lines prevented any conclusion about the advantages or disadvantages of uptake hydrogenase activity for plant growth, but in general, cultivars with high uptake hydrogenase activity and low net H2 evolution grew more slowly than those evolving large amounts of H2.

Nitrogen fixation and hydrogen metabolism by Rhizobium leguminosarum isolates in pea root nodules

1981 ◽  
Vol 27 (10) ◽  
pp. 1028-1034 ◽  
Author(s):  
Louise M. Nelson ◽  
J. J. Child
Keyword(s):  
Relative Efficiency ◽  
Rhizobium Leguminosarum ◽  
High Efficiency ◽  
Root Nodules ◽  
Dry Weight ◽  
Hydrogenase Activity ◽  
Uptake Hydrogenase ◽  
Hydrogen Metabolism ◽  
N Content ◽  
Significant Uptake

A survey of 108 isolates of Rhizobium leguminosarum was conducted to determine the variation in H2 uptake and relative efficiency of N2 fixation in Pisum sativum L. root nodules and the relation of relative efficiency to plant dry weight and N content. Only 14 of the isolates exhibited significant uptake hydrogenase activity and none of these had sufficient hydrogenase activity to recycle all of the H2 produced by nitrogenase. In 74 of the isolates tested relative efficiencies of N2 fixation were less than 0.60.Twenty-nine of the isolates were ineffective, since total plant N at harvest did not differ significantly from uninoculated controls. The remaining 79 effective isolates could be divided into low-and high-efficiency groups. Plants which were inoculated with isolates from the two groups and harvested after 4 weeks did not differ significantly in plant dry weight or N content. Among the isolates with high relative efficiency of N2 fixation, two groups could be recognized: one possessing significant uptake hydrogenase activity, the other lacking hydrogenase activity but in which H2 evolution was low. Although the two groups did not differ with respect to plant dry weight or N content, the identification of this latter group may be of some significance for optimizing the efficiency of N2 fixation.

scholarly journals Development and Partial Characterization of Nearly Isogenic Pea Lines (Pisum sativum L.) that Alter Uptake Hydrogenase Activity in Symbiotic Rhizobium

1990 ◽  
Vol 92 (4) ◽  
pp. 983-989 ◽  
Author(s):  
Donald A. Phillips ◽  
Yoram Kapulnik ◽  
Eulogio J. Bedmar ◽  
Cecillia M. Joseph
Keyword(s):  
Pisum Sativum ◽  
Partial Characterization ◽  
Hydrogenase Activity ◽  
Uptake Hydrogenase ◽  
Pisum Sativum L

Acetylene reduction and hydrogen evolution by nitrogenase in a Rhizobium–legume symbiosis

1983 ◽  
Vol 61 (3) ◽  
pp. 780-785 ◽  
Author(s):  
Scott A. Edie
Keyword(s):  
Hydrogen Evolution ◽  
Acetylene Reduction ◽  
Rhizobium Leguminosarum ◽  
High Light ◽  
Hydrogenase Activity ◽  
Uptake Hydrogenase ◽  
Vegetative Phase ◽  
Pisum Sativum L ◽  
Legume Symbiosis ◽  
The Relationship

The relationship between acetylene reduction and hydrogen evolution in air was examined in peas (Pisum sativum L. cv. Alaska) inoculated with Rhizobium leguminosarum strain 3740, which lacked uptake hydrogenase activity (Hup−). In the absence of a system for recycling hydrogen, changes in the relative efficiency of N2 fixation (RE), which is defined as RE = 1 − (hydrogen evolved in air)/(acetylene reduced), presumably reflect an altered capacity of nitrogenase to allocate electrons between protons and N2. The RE of plants grown without combined nitrogen declined during the vegetative phase of growth and increased after flowering. Continuous high light or elevated CO2 conditions in the absence of nitrate maintained throughout ontogeny accentuated decreases in RE. When nitrate was present in the growth medium declines in RE during the vegetative phase were lessened. These results are consistent with the concept that the electron allocation coefficient of nitrogenase varies in the absence of uptake hydrogenase activity.

Molecular characterization and identification of plant growth promoting endophytic bacteria isolated from the root nodules of pea (Pisum sativum L.)

2013 ◽  
Vol 30 (2) ◽  
pp. 719-725 ◽  
Author(s):  
Mohsin Tariq ◽  
Sohail Hameed ◽  
Tahira Yasmeen ◽  
Mehwish Zahid ◽  
Marriam Zafar
Keyword(s):  
Pisum Sativum ◽  
Plant Growth ◽  
Molecular Characterization ◽  
Endophytic Bacteria ◽  
Root Nodules ◽  
Pisum Sativum L ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals NodC-based evaluation of the occurrence of bacteria in nodules of Pisum sativum isolated on YEM agar

2019 ◽  
Vol 70 (1) ◽  
pp. 59-67
Author(s):  
Anna Lenart-Boroń ◽  
Tadeusz Zając ◽  
Piotr Mateusz Boroń ◽  
Agnieszka Klimek-Kopyra
Keyword(s):  
Pisum Sativum ◽  
Rhizobium Leguminosarum ◽  
Reference Strain ◽  
Root Nodules ◽  
Bacterial Species ◽  
Soil Science ◽  
Associated Bacteria ◽  
Plant Cultivation ◽  
Yeast Extract Mannitol ◽  
Diverse Community

SummaryThe bacterial nodulation (nod) genes are essential in the formation process of root nodules. This study was aimed to verify the occurrence of nodule-associated bacteria in two pea varieties (“Tarchalska” and “Klif ”) inoculated withRhizobiuminoculants – Nitragine™ and a noncommercial one produced by the Polish Institute of Soil Science and Plant Cultivation (IUNG). The number of colonies isolated on yeast extract mannitol (YEM) agar from the nodules of “Klif ” inoculated with IUNG inoculants was significantly higher than the number of colonies isolated from other variants. Species identification was based on sequencing of 16S rDNA, which revealed that despite careful sterilization of nodules, sequences of other bacterial species were detected. Among them, one sequence belonged toRhizobium leguminosarum(isolated from IUNG inoculant). To assess the presence of nodulation-capableRhizobium, amplification of thenodCgene was performed, which revealed that of 29 samples, 19 were positive. The remaining isolates, including reference strain and bacteria isolated from Nitragine™, lacked this gene. The results show that pea nodules harbor a very diverse community of bacteria. The lack ofnodCgene in some strains isolated from plants inoculated with Nitragine™ and with IUNG inoculant proves that even ifR. leguminosarumare abundant, they may not be efficient in nodulation.

Selection of Rhizobium leguminosarum bv. viceae strains for inoculation of Pisum sativum L. cultivars: Analysis of symbiotic efficiency and nodulation competitiveness

1995 ◽  
Vol 172 (2) ◽  
pp. 189-198 ◽  
Author(s):  
A. N. Fesenko ◽  
N. A. Provorov ◽  
Irina F. Orlova ◽  
V. P. Orlov ◽  
B. V. Simarov
Keyword(s):  
Pisum Sativum ◽  
Rhizobium Leguminosarum ◽  
Symbiotic Efficiency ◽  
Pisum Sativum L ◽  
Nodulation Competitiveness ◽  
Selection Of

scholarly journals Heterologous Complementation Reveals a Specialized Activity for BacA in the Medicago–Sinorhizobium meliloti Symbiosis

2017 ◽  
Vol 30 (4) ◽  
pp. 312-324 ◽  
Author(s):  
George C. diCenzo ◽  
Maryam Zamani ◽  
Hannah N. Ludwig ◽  
Turlough M. Finan
Keyword(s):  
Pisum Sativum ◽  
Sinorhizobium Meliloti ◽  
Rhizobium Leguminosarum ◽  
Root Nodules ◽  
Specific Interaction ◽  
Model System ◽  
Single Model ◽  
Leguminous Plants ◽  
Melilotus Alba ◽  
Developmental Progression

The bacterium Sinorhizobium meliloti Rm2011 forms N2-fixing root nodules on alfalfa and other leguminous plants. The pSymB chromid contains a 110-kb region (the ETR region) showing high synteny to a chromosomally located region in Sinorhizobium fredii NGR234 and related rhizobia. We recently introduced the ETR region from S. fredii NGR234 into the S. meliloti chromosome. Here, we report that, unexpectedly, the S. fredii NGR234 ETR region did not complement deletion of the S. meliloti ETR region in symbiosis with Medicago sativa. This phenotype was due to the bacA gene of NGR234 not being functionally interchangeable with the S. meliloti bacA gene during M. sativa symbiosis. Further analysis revealed that, whereas bacA genes from S. fredii or Rhizobium leguminosarum bv. viciae 3841 failed to complement the Fix− phenotype of a S. meliloti bacA mutant with M. sativa, they allowed for further developmental progression prior to a loss of viability. In contrast, with Melilotus alba, bacA from S. fredii and R. leguminosarum supported N2 fixation by a S. meliloti bacA mutant. Additionally, the S. meliloti bacA gene can support N2 fixation of a R. leguminosarum bacA mutant during symbiosis with Pisum sativum. A phylogeny of BacA proteins illustrated that S. meliloti BacA has rapidly diverged from most rhizobia and has converged toward the sequence of pathogenic genera Brucella and Escherichia. These data suggest that the S. meliloti BacA has evolved toward a specific interaction with Medicago and highlights the limitations of using a single model system for the study of complex biological topics.

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