scholarly journals Nitrate Effects on Nitrate Reductase Activity and Nitrite Reductase mRNA Levels in Maize Suspension Cultures

1989 ◽  
Vol 90 (3) ◽  
pp. 962-967 ◽  
Author(s):  
Laura S. Privalle ◽  
Kristi N. Lahners ◽  
Martha A. Mullins ◽  
Steven Rothstein
Keyword(s):  
Nitrate Reductase ◽  
Nitrite Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Suspension Cultures ◽  
Mrna Levels ◽  
Reductase Mrna

Effects of oxygen on each step of denitrification on Pseudomonas nautica

1989 ◽  
Vol 35 (11) ◽  
pp. 1061-1064 ◽  
Author(s):  
P. Bonin ◽  
M. Gilewicz ◽  
J. C. Bertrand
Keyword(s):  
Nitrous Oxide ◽  
Nitrate Reductase ◽  
Oxygen Concentration ◽  
Nitrite Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
A Value ◽  
Nitrite Reductase Activity ◽  
Effect Of Oxygen

Studies on the effect of oxygen on denitrification have shown that denitrification on Pseudomonas nautica 617 can take place in the presence of oxygen. The enzymes associated with denitrification are affected differently with respect to oxygen concentration. Nitrate reductase was less sensitive toward oxygen than nitrite and nitrous oxide reductases. Nitrate reductase activity was completely blocked at an oxygen concentration greater than 4.05 mg/L, compared with 2.15 and 0.25 mg/L for nitrite and nitrous oxide reductases, respectively. After an aerobic–anaerobic shift, nitrate reductase activity remained unchanged whereas the rate of nitrite reductase activity rose to a value only 20% that of the original rate.Key words: denitrification, oxygen, Pseudomonas.

Nitrate reductase activity in Paul's scarlet rose suspension cultures and the differential role of nitrate and molybdenum in induction

Planta ◽  
1978 ◽  
Vol 141 (2) ◽  
pp. 183-189 ◽  
Author(s):  
R. W. Jones ◽  
A. J. Abbott ◽  
E. J. Hewitt ◽  
G. R. Best ◽  
E. F. Watson
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Suspension Cultures

Nitrate reductase activity and nitrogenous gas evolution from heterotrophic, photomixotrophic and photoautotrophic soybean suspension cultures

1984 ◽  
Vol 34 (1-2) ◽  
pp. 145-152 ◽  
Author(s):  
Richard S. Nelson ◽  
Michael E. Horn ◽  
James E. Harper ◽  
Jack M. Widholm
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Suspension Cultures ◽  
Gas Evolution

Short-term effects of nitrate on nitrate reductase activity and symbiotic dinitrogen fixation in faba bean and pea

1988 ◽  
Vol 66 (8) ◽  
pp. 1639-1645 ◽  
Author(s):  
Francois-P. Chalifour ◽  
Louise M. Nelson
Keyword(s):  
Nitrate Reductase ◽  
Faba Bean ◽  
Nitrite Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Inhibitory Effects ◽  
Short Term ◽  
Pisum Sativum L ◽  
Vicia Faba L ◽  
Legume Symbiosis

The effects of a short-term supply of combined nitrogen (N) on the Rhizobium–legume symbiosis were studied in faba bean (Vicia faba L.) and pea (Pisum sativum L.) nodulated by R. leguminosarum biovar viceae isolate 175F5 or 175F19 and subjected to increasing levels of [Formula: see text] (0, 5, 10, or 20 mol∙m−3) from 28 to 36 days after planting. Trends in N2-fixing activity (acetylene reduction) showed that faba bean was more tolerant to [Formula: see text] than pea with isolate 175F5 but not with isolate 175F19. Nitrite reductase activities in the leaf, root, and nodule cytosol fractions were severalfold higher than nitrate reductase activities for both hosts. The levels of these enzymes in the nodule cytosol, the absence of bacteroid nitrate reductase and the lack of induction of bacteroid nitrite reductase in response to [Formula: see text] addition are consistent with the lack of [Formula: see text] accumulation in nodules of both hosts. Therefore, it is unlikely that the inhibitory effects of [Formula: see text] on N2 fixation are due to [Formula: see text] inhibition of nitrogenase. The relative levels of nitrate reductase activity in the root and nodule cytosol fractions were, respectively, higher and lower in the two faba bean symbioses ([Formula: see text] tolerant) and in the pea–175F5 symbiosis ([Formula: see text] sensitive) than in the pea–175F19 symbiosis ([Formula: see text] tolerant).

Nitrate reductase activity and growth in Paul's Scarlet rose suspension cultures in relation to nitrogen source and molybdenum

Planta ◽  
1976 ◽  
Vol 133 (1) ◽  
pp. 27-34 ◽  
Author(s):  
R. W. Jones ◽  
A. J. Abbott ◽  
E. J. Hewitt ◽  
D. M. James ◽  
G. R. Best
Keyword(s):  
Nitrate Reductase ◽  
Nitrogen Source ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Suspension Cultures

scholarly journals Effect of tungsten on nitrate and nitrite reductases in Azospirillum brasilense Sp7

1991 ◽  
Vol 37 (10) ◽  
pp. 744-750 ◽  
Author(s):  
Christian Chauret ◽  
Roger Knowles
Keyword(s):  
Nitrate Reductase ◽  
Nitrite Reductase ◽  
Azospirillum Brasilense ◽  
Nitrate Reductase Activity ◽  
Paracoccus Denitrificans ◽  
Reductase Activity ◽  
Whole Cells ◽  
Azospirillum Brasilense Sp7 ◽  
Nitrite Reductase Activity ◽  
Nitrate And Nitrite

Tungstate, at concentrations that completely suppressed nitrate reductase activity in Paracoccus denitrificans, caused only partial inhibition of nitrate reductase in Azospirillum brasilense Sp7. Nitrate reductase activity in cell-free extracts was much more sensitive than whole cells to tungstate, suggesting that there may be a barrier to its transport. Nitrite reductase activity was partially inhibited by tungstate in both whole cells and cell-free extracts. Azospirillum brasilense apparently scavenged enough contaminating molybdenum from molybdenum-limited medium to allow maximum nitrate reductase activity, which was not stimulated by added molybdate. Cells grown in molybdenum-depleted medium could not reduce nitrate. Nitrate concentrations less than 0.25 mM inhibited activity, but not synthesis, of nitrite reductase and caused significant accumulation of nitrite during reduction of nitrate. Key words: Azospirillum brasilense, Paracoccus denitrificans, nitrate reductase, nitrite reductase, tungsten, molybdenum, denitrification.

scholarly journals The effect of various light conditions and different nitrogen forms on nitrogen metabolism in pepper fruits

2012 ◽  
Vol 24 (2) ◽  
pp. 153-160 ◽  
Author(s):  
Anna Kołton ◽  
Renata Wojciechowska ◽  
Maria Leja
Keyword(s):  
Nitrate Reductase ◽  
Nitrogen Metabolism ◽  
Nitrite Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Red Pepper ◽  
Ammonium Ion ◽  
Light Conditions ◽  
Nitrogen Forms ◽  
Ammonium Ions

Abstract The ‘Spartacus’ F1 sweet pepper was grown in a plastic tunnel on rockwool during 2006-2008. A fertigation technique was used for water and fertiliser application. The tunnel was divided into two parts covered with different plastic films. The first part of the tunnel was covered with a film that transmitted less light than the film covering the second part. In both parts of the tunnel, the plants were divided into two groups. One group of plants was fertilised with just nitrate nitrogen (100% N-NO3) and the other one with three forms of nitrogen (N-NO3:N-NH4:N-NH2 in a ratio of 50:13:37). Fruits were harvested mature green and red. Concentrations of nitrate and ammonium ions as well as total nitrogen and free amino acids were analysed in the plant material. Nitrate and nitrite reductase activities were also investigated, and dry matter content and soluble sugars were also determined. Higher light intensity increased nitrate concentration in red pepper fruits but decreased ammonium ion content. These tendencies were not as obvious in green fruits. In most cases, red fruits fertilised with three nitrogen forms accumulated more nitrates than those fertilised with N-NO3. This observation was similar in the case of green fruits. In most cases, pepper fruits accumulated more ammonium ions in the case of N-NO3 fertilisation than when three forms of nitrogen were applied, but the differences were not always statistically significant. Higher nitrate reductase activity was observed in the case of better light conditions as well as mixed nitrogen fertilisation in red pepper fruits. No differences were observed in the case of nitrite reductase activity between fruits harvested from various treatments in red and also green fruits, with some exceptions. The green fruits of pepper had higher nitrate reductase activity than the red ones. It can be summarised that various light conditions influenced the nitrogen metabolism of pepper fruits as well as the different nitrogen forms applied with fertilisers.

Nitrate assimilation in suspension cultures of Paul's scarlet rose

1980 ◽  
Vol 58 (9) ◽  
pp. 1088-1094 ◽  
Author(s):  
Douglas B. Jordan ◽  
John S. Fletcher
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Nitrate Assimilation ◽  
Suspension Cultures ◽  
Fresh Weight ◽  
Assay Medium ◽  
Enzyme Extraction ◽  
Cell Age

Nitrate reductase activity, the assimilation of NO3−, and growth were studied in suspension cultures of Rosa cv. Paul's Scarlet grown in media with either 24 mM NO3−,or with 24 mM NO3− + 0.91 mM NH4+.Ammonium partially repressed the development of nitrate reductase during the first 4 days of growth and the degree of repression was more pronounced when cells were provided with lower concentrations of NO3−. The repression of nitrate reductase by NH4+ was only observed when casein was used during enzyme extraction. The repression of nitrate reductase activity by NH4+ was not recognized in earlier work when no casein was used.The presence of casein in the extraction or assay medium increased the total recoverable nitrate reductase activity. The enhancement was differential depending on cell age with the greatest influence being on young and old cells where 100 and 400% enhancements were observed, respectively.In vitro nitrate reductase activity correlated well with NO3− assimilation. Over 14 days of growth, the ratio of total nitrate reductase activity to the total amount of NO3− reduced was 3.8 for cultures grown with or without NH4+. Cultures grown with NH4+ achieved 80% more fresh weight and reduced 70% more NO3− over 14 days than cultures grown without NH4+.

scholarly journals Ammonium Influence on the Growth and Nitrate Reductase Activity of Paul's Scarlet Rose Suspension Cultures

1976 ◽  
Vol 58 (2) ◽  
pp. 152-155 ◽  
Author(s):  
Bibekananda Mohanty ◽  
John S. Fletcher
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Suspension Cultures
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