scholarly journals Phospholipase D Activation Correlates with Microtubule Reorganization in Living Plant Cells

2003 ◽  
Vol 15 (11) ◽  
pp. 2666-2679 ◽  
Author(s):  
Pankaj Dhonukshe ◽  
Ana M. Laxalt ◽  
Joachim Goedhart ◽  
Theodorus W. J. Gadella ◽  
Teun Munnik
Keyword(s):  
Phospholipase D ◽  
Plant Cells ◽  
Living Plant

Confocal microscopy of the cytoskeleton in living plant cells following microinjection of fluorescent probes

1993 ◽  
Vol 51 ◽  
pp. 130-131
Author(s):  
Ann Cleary
Keyword(s):  
Cell Division ◽  
Fluorescent Probes ◽  
Actin Filaments ◽  
Intracellular Transport ◽  
Cell Structure ◽  
Plant Cells ◽  
Cell Plate ◽  
Cell Cortex ◽  
Living Plant ◽  
Rhodamine Phalloidin

Microinjection of fluorescent probes into living plant cells reveals new aspects of cell structure and function. Microtubules and actin filaments are dynamic components of the cytoskeleton and are involved in cell growth, division and intracellular transport. To date, cytoskeletal probes used in microinjection studies have included rhodamine-phalloidin for labelling actin filaments and fluorescently labelled animal tubulin for incorporation into microtubules. From a recent study of Tradescantia stamen hair cells it appears that actin may have a role in defining the plane of cell division. Unlike microtubules, actin is present in the cell cortex and delimits the division site throughout mitosis. Herein, I shall describe actin, its arrangement and putative role in cell plate placement, in another material, living cells of Tradescantia leaf epidermis.The epidermis is peeled from the abaxial surface of young leaves usually without disruption to cytoplasmic streaming or cell division. The peel is stuck to the base of a well slide using 0.1% polyethylenimine and bathed in a solution of 1% mannitol +/− 1 mM probenecid.

scholarly journals Behavior of Microtubules in Living Plant Cells

1996 ◽  
Vol 112 (2) ◽  
pp. 455-461 ◽  
Author(s):  
P. K. Hepler ◽  
J. M. Hush
Keyword(s):  
Plant Cells ◽  
Living Plant

Effectors of biotrophic fungal plant pathogens

2010 ◽  
Vol 37 (10) ◽  
pp. 913 ◽  
Author(s):  
Pamela H. P. Gan ◽  
Maryam Rafiqi ◽  
Adrienne R. Hardham ◽  
Peter N. Dodds
Keyword(s):  
Plant Tissue ◽  
Fungal Pathogen ◽  
Plant Pathogens ◽  
Plant Cells ◽  
Host Cells ◽  
Plant Proteins ◽  
Living Plant ◽  
Host Cytoplasm ◽  
Fungal Plant Pathogens ◽  
Biotrophic Fungi

Plant pathogenic biotrophic fungi are able to grow within living plant tissue due to the action of secreted pathogen proteins known as effectors that alter the response of plant cells to pathogens. The discovery and identification of these proteins has greatly expanded with the sequencing and annotation of fungal pathogen genomes. Studies to characterise effector function have revealed that a subset of these secreted pathogen proteins interact with plant proteins within the host cytoplasm. This review focuses on the effectors of intracellular biotrophic and hemibiotrophic fungal plant pathogens and summarises advances in understanding the roles of these proteins in disease and in elucidating the mechanism of fungal effector uptake into host cells.

scholarly journals Quantification of Förster resonance energy transfer by monitoring sensitized emission in living plant cells

2013 ◽  
Vol 4 ◽  
Author(s):  
Sara M. Müller ◽  
Helena Galliardt ◽  
Jessica Schneider ◽  
B. George Barisas ◽  
Thorsten Seidel
Keyword(s):  
Energy Transfer ◽  
Resonance Energy Transfer ◽  
Plant Cells ◽  
Resonance Energy ◽  
Forster Resonance Energy Transfer ◽  
Förster Resonance Energy Transfer ◽  
Living Plant ◽  
Förster Resonance

Primary cell wall metabolism: tracking the careers of wall polymers in living plant cells

2004 ◽  
Vol 161 (3) ◽  
pp. 641-675 ◽  
Author(s):  
Stephen C. Fry
Keyword(s):  
Cell Wall ◽  
Plant Cells ◽  
Primary Cell ◽  
Primary Cell Wall ◽  
Cell Wall Metabolism ◽  
Living Plant

Recording mitotic chromosome cycle induction in living plant cells

PROTOPLASMA ◽  
1981 ◽  
Vol 107 (1-2) ◽  
pp. 189-194
Author(s):  
C. de la Torre ◽  
M. L. Moreno
Keyword(s):  
Mitotic Chromosome ◽  
Plant Cells ◽  
Living Plant ◽  
Chromosome Cycle

Förster Resonance Energy Transfer Measurements in Living Plant Cells

10.3791/62758 ◽  
2021 ◽  
Author(s):  
Sonja Michèle Schmidtpott ◽  
Thorsten Seidel
Keyword(s):  
Energy Transfer ◽  
Resonance Energy Transfer ◽  
Plant Cells ◽  
Resonance Energy ◽  
Living Plant

Multiphoton microspectroscopy in living plant cells

2003 ◽  
Author(s):  
Jan-Willem Borst ◽  
Mark A. Hink ◽  
Arie van Hoek ◽  
A. J. W. G. Visser
Keyword(s):  
Plant Cells ◽  
Living Plant
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