scholarly journals Roles for Arabidopsis CAMTA Transcription Factors in Cold-Regulated Gene Expression and Freezing Tolerance

2009 ◽  
Vol 21 (3) ◽  
pp. 972-984 ◽  
Author(s):  
Colleen J. Doherty ◽  
Heather A. Van Buskirk ◽  
Susan J. Myers ◽  
Michael F. Thomashow
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Freezing Tolerance ◽  
Regulated Gene Expression

scholarly journals Abscisic Acid-Regulated Gene Expression in Relation to Freezing Tolerance in Alfalfa

1988 ◽  
Vol 87 (2) ◽  
pp. 468-473 ◽  
Author(s):  
Shyam S. Mohapatra ◽  
Ronald J. Poole ◽  
Rajinder S. Dhindsa
Keyword(s):  
Gene Expression ◽  
Abscisic Acid ◽  
Freezing Tolerance ◽  
Regulated Gene Expression

scholarly journals Control of oocyte release by progesterone receptor-regulated gene expression

2009 ◽  
Vol 7 (1) ◽  
pp. nrs.07012 ◽  
Author(s):  
Rebecca L. Robker ◽  
Lisa K. Akison ◽  
Darryl L. Russell
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Progesterone Receptor ◽  
Granulosa Cells ◽  
Cellular Mechanisms ◽  
Ovarian Granulosa Cells ◽  
Regulated Gene Expression ◽  
Cell Expression ◽  
Kinase A ◽  
Transcriptional Target

The progesterone receptor (PGR) is a nuclear receptor transcription factor that is essential for female fertility, in part due to its control of oocyte release from the ovary, or ovulation. In all mammals studied to date, ovarian expression of PGR is restricted primarily to granulosa cells of follicles destined to ovulate. Granulosa cell expression of PGR is induced by the pituitary Luteinizing Hormone (LH) surge via mechanisms that are not entirely understood, but which involve activation of Protein Kinase A and modification of Sp1/Sp3 transcription factors on the PGR promoter. Null mutations for PGR or treatment with PGR antagonists block ovulation in all species analyzed, including humans. The cellular mechanisms by which PGR regulates ovulation are currently under investigation, with several downstream pathways having been identified as PGR-regulated and potentially involved in follicular rupture. Interestingly, none of these PGR-regulated genes has been demonstrated to be a direct transcriptional target of PGR. Rather, in ovarian granulosa cells, PGR may act as an inducible coregulator for constitutively bound Sp1/Sp3 transcription factors, which are key regulators for a discrete cohort of ovulatory genes.

The Role of GAMYB Transcription Factors in GA-Regulated Gene Expression

2003 ◽  
Vol 22 (2) ◽  
pp. 176-184 ◽  
Author(s):  
Fiona J. Woodger ◽  
Anthony Millar ◽  
Fiona Murray ◽  
John V. Jacobsen ◽  
Frank Gubler
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Regulated Gene Expression

120. OXYGEN REGULATED GENE EXPRESSION IN MOUSE CUMULUS CELLS

2009 ◽  
Vol 21 (9) ◽  
pp. 39
Author(s):  
K. Tam ◽  
K. Banwell ◽  
D. Froiland ◽  
D. Russell ◽  
K. Kind ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Oxygen Concentration ◽  
In Vitro Maturation ◽  
Target Genes ◽  
Cumulus Cells ◽  
Differentially Expressed ◽  
Oxygen Levels ◽  
Regulated Gene Expression

Hypoxia inducible factors (HIFs) are heterodimeric transcription factors that mediate the expression of a range of genes in response to low oxygen. Previously we showed that subsequent developmental outcomes were influenced by oxygen levels during in vitro maturation. The aim of the current study was to examine the effects of varying oxygen concentration during in vitro maturation of mouse COCs on expression of HIF target genes in the cumulus cells. I mmature COCs were collected from the ovaries of eCG-stimulated CBAB6F1 females (21 d) and cultured for 17-18 h under 2, 5 or 20% O2. Hyaluronidase-treated and recovered cumulus cells were collected and mRNA extracted for analysis. A microarray approach (Affymetrix 430_2) was used to identify genes in cumulus cells that were differentially expressed under varying oxygen concentrations (2, 5, 10 and 20%). This revealed 218 differentially expressed probes, of which 34 were up-regulated with decreasing oxygen levels. The great majority of these were classified as HIF-regulated genes. Specific analysis from real time RT-PCR of HIF regulated target genes Slc2a1, Ldha, Pgk1, Eno1, Ndrg1, Bnip3 were all significantly up-regulated (by at least 5–fold) when cells were cultured at 2% or 5% oxygen, when compared to 20% oxygen. Hif-1a mRNA decreased when cumulus cells were cultured in 2%, compared to 20% oxygen. This study demonstrates that cumulus cell gene expression is influenced by oxygen concentration, and suggests that these effects are mediated by the HIF transcription factors.

scholarly journals Formation of an Active Tissue-Specific Chromatin Domain Initiated by Epigenetic Marking at the Embryonic Stem Cell Stage

2005 ◽  
Vol 25 (5) ◽  
pp. 1804-1820 ◽  
Author(s):  
Henrietta Szutorisz ◽  
Claudia Canzonetta ◽  
Andrew Georgiou ◽  
Cheok-Man Chow ◽  
László Tora ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
B Cells ◽  
B Cell ◽  
Histone H3 ◽  
Embryonic Stem ◽  
Chromatin Domain ◽  
Cell Stage ◽  
General Transcription Factors ◽  
Regulated Gene Expression

ABSTRACT The differentiation potential of stem cells is determined by the ability of these cells to establish and maintain developmentally regulated gene expression programs that are specific to different lineages. Although transcriptionally potentiated epigenetic states of genes have been described for haematopoietic progenitors, the developmental stage at which the formation of lineage-specific gene expression domains is initiated remains unclear. In this study, we show that an intergenic cis-acting element in the mouse λ5-VpreB1 locus is marked by histone H3 acetylation and histone H3 lysine 4 methylation at a discrete site in embryonic stem (ES) cells. The epigenetic modifications spread from this site toward the VpreB1 and λ5 genes at later stages of B-cell development, and a large, active chromatin domain is established in pre-B cells when the genes are fully expressed. In early B-cell progenitors, the binding of haematopoietic factor PU.1 coincides with the expansion of the marked region, and the region becomes a center for the recruitment of general transcription factors and RNA polymerase II. In pre-B cells, E2A also binds to the locus, and general transcription factors are distributed across the active domain, including the gene promoters and the intergenic region. These results suggest that localized epigenetic marking is important for establishing the transcriptional competence of the λ5 and VpreB1 genes as early as the pluripotent ES cell stage.

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