Purification, crystallization and preliminary crystallographic study of a Kunitz-type trypsin inhibitor from Delonix regia seeds
1999 ◽
Vol 55
(9)
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pp. 1611-1613
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Keyword(s):
X Ray
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The Kunitz-type trypsin inhibitor from seeds of Flamboyant (Delonix regia) has been purified to homogeneity and plate-like crystals suitable for X-ray analysis have been grown by the hanging-drop method using PEG 6000 as a precipitant. The crystals belong to space group P212121 with unit-cell parameters a = 32.15, b = 69.39, c = 72.54 Å. X-ray diffraction data have been collected to 2.95 Å resolution. The structure has been solved by molecular replacement using the known structures of trypsin inhibitors from Erythrina caffra seeds (PDB code 1tie) and from soya beans (Glycine max; PDB code 1ba7) as search models.
2015 ◽
Vol 71
(4)
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pp. 466-470
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2018 ◽
Vol 74
(9)
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pp. 543-548
2016 ◽
Vol 72
(9)
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pp. 667-671
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2009 ◽
Vol 65
(11)
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pp. 1179-1181
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