Studies on intracellular structures of COS cells by X-ray microscopy

1998 ◽  
Vol 5 (3) ◽  
pp. 1105-1107 ◽  
Author(s):  
Akitsugu Yamamoto ◽  
Ryuichi Masaki ◽  
Peter Guttmann ◽  
Günter Schmahl ◽  
Hiroshi Kihara
Keyword(s):  
Electron Microscopy ◽  
Storage Ring ◽  
Multivesicular Bodies ◽  
Electron Storage ◽  
X Ray ◽  
Cos Cells ◽  
Dense Granules ◽  
Lysosomal System

COS-7 cells, fixed with glutaraldehyde, were studied using the transmission X-ray microscope at the electron storage ring BESSY, Berlin. The border of the cell, the nucleus, nucleoli and mitochondria of the cells were clearly visualized with the X-ray microscope. In addition, we found many X-ray dense granules preferentially located around the nucleus. Electron microscopy showed that numerous multivesicular bodies, whose structures belong to the endosome–lysosomal system, were present around the nucleus. The size and localization patterns of the X-ray dense granules were quite similar to those of multivesicular bodies. These results strongly suggest that the X-ray dense granules are multivesicular bodies.

scholarly journals Development of a source of femtosecond X-ray pulses based on the electron storage ring

2003 ◽  
Author(s):  
A. Zholents ◽  
J. Byrd ◽  
S. Chattopadhyay ◽  
H. Chong ◽  
T.E. Glover ◽  
...  
Keyword(s):  
Storage Ring ◽  
Electron Storage ◽  
X Ray

X-ray microscopy: experimental results with the Göttingen X-ray microscope at the electron storage ring BESSY in Berlin

1985 ◽  
Vol 138 (3) ◽  
pp. 279-284 ◽  
Author(s):  
G. Schmahl ◽  
B. Niemann ◽  
D. Rudolph ◽  
P. Guttmann ◽  
V. Sarafis
Keyword(s):  
Storage Ring ◽  
Experimental Results ◽  
Electron Storage ◽  
X Ray

scholarly journals A MORPHOLOGIC AND CYTOCHEMICAL STUDY ON THE GREAT ALVEOLAR CELL

1966 ◽  
Vol 14 (12) ◽  
pp. 884-897 ◽  
Author(s):  
SERGEI P. SOROKIN
Keyword(s):  
Electron Microscopy ◽  
Periodic Acid ◽  
Light And Electron Microscopy ◽  
Multivesicular Bodies ◽  
Alveolar Cells ◽  
Periodic Acid Schiff ◽  
Alveolar Cell ◽  
Cytochemical Study ◽  
Lysosomal System ◽  
Chloroform Methanol

Lungs from marsupials, bats and rodents were studied by light and electron microscopy. In all three groups, the great alveolar cells exhibit similar morphologic and cytochemical characteristics. Cytoplasmic vacuoles seen in these cells by light microscopy correspond to cytosomes that are demonstrable in them by electron microscopy. Such cytosomes are osmiophilic, periodic acid-Schiff-positive and stainable with Sudan black after acetone extraction. After fixation in a mixture of aldehydes, followed by extraction in chloroform-methanol and postfixation in osmium tetroxide, cytosomes lose their osmiophilia. The cytoplasm of the great alveolar cell is notable for a loosely ordered granular endoplasmic reticulum, an extensive Golgi apparatus and numerous multivesicular bodies. Many forms transitional in appearance between multivesicular bodies and cytosomes are present. In these, osmiophilic matter occupies the intervesicular space. It is proposed that these bodies are the precursors of cytosomes. The cytosomes are interpreted to be products of the "lysosomal" system in this cell. Ultimately they are secreted onto the alveolar surface.

Synchrotron radiation possibilities at CHEER

1981 ◽  
Vol 59 (11) ◽  
pp. 1811-1816
Author(s):  
J. B. A. Mitchell ◽  
J. Wm. McGowan ◽  
G. M. Bancroft
Keyword(s):  
Synchrotron Radiation ◽  
Storage Ring ◽  
Radiation Spectrum ◽  
Far Infrared ◽  
Critical Energy ◽  
X Rays ◽  
Electron Storage ◽  
Materials Analysis ◽  
X Ray

The 10 GeV CHEER electron storage ring will produce more than 1 MW of synchrotron radiation per turn with photon energies extending from the far infrared to the hard X-ray region. The critical energy of the synchrotron radiation spectrum will be 22 keV.Applications of hard X-rays to materials analysis are described and a discussion of some of the problems which will be encountered in implementing a synchrotron radiation facility at CHEER is presented.

scholarly journals Generation of Femtosecond Soft X-Ray Pulse by Interaction between Laser and Electron Beam in an Electron Storage Ring.

2002 ◽  
Vol 30 (9) ◽  
pp. 531-537 ◽  
Author(s):  
Takahiro INOUE ◽  
Shuji MIYAMOTO ◽  
Sho AMANO ◽  
Takayasu MOCHIZUKI ◽  
Mitsuyasu YATSUZUKA
Keyword(s):  
Electron Beam ◽  
Storage Ring ◽  
Electron Storage ◽  
X Ray
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