scholarly journals The TELL automatic sample changer for macromolecular crystallography

2020 ◽  
Vol 27 (3) ◽  
pp. 860-863 ◽  
Author(s):  
Isabelle Martiel ◽  
Dominik Buntschu ◽  
Nathalie Meier ◽  
Alexandre Gobbo ◽  
Ezequiel Panepucci ◽  
...  
Keyword(s):  
Light Source ◽  
High Throughput ◽  
Storage Capacity ◽  
Paul Scherrer Institute ◽  
Macromolecular Crystallography ◽  
Exchange System ◽  
Automatic Sample Changer ◽  
Sample Changer ◽  
Swiss Light Source ◽  
Paul Scherrer

In this paper, the design and functionalities of the high-throughput TELL sample exchange system for macromolecular crystallography is presented. TELL was developed at the Paul Scherrer Institute with a focus on speed, storage capacity and reliability to serve the three macromolecular crystallography beamlines of the Swiss Light Source, as well as the SwissMX instrument at SwissFEL.

scholarly journals DA+data acquisition and analysis software at the Swiss Light Source macromolecular crystallography beamlines

2018 ◽  
Vol 25 (1) ◽  
pp. 293-303 ◽  
Author(s):  
Justyna Aleksandra Wojdyla ◽  
Jakub W. Kaminski ◽  
Ezequiel Panepucci ◽  
Simon Ebner ◽  
Xiaoqiang Wang ◽  
...  
Keyword(s):  
Data Acquisition ◽  
Light Source ◽  
Full Potential ◽  
Macromolecular Crystallography ◽  
Automatic Data ◽  
Online Processing ◽  
Data Acquisition Software ◽  
Swiss Light Source ◽  
Paul Scherrer ◽  
Acquisition Software

Data acquisition software is an essential component of modern macromolecular crystallography (MX) beamlines, enabling efficient use of beam time at synchrotron facilities. Developed at the Paul Scherrer Institute, theDA+data acquisition software is implemented at all three Swiss Light Source (SLS) MX beamlines.DA+consists of distributed services and components written in Python and Java, which communicateviamessaging and streaming technologies. The major components ofDA+are the user interface, acquisition engine, online processing and database. Immediate data quality feedback is achieved with distributed automatic data analysis routines. The software architecture enables exploration of the full potential of the latest instrumentation at the SLS MX beamlines, such as the SmarGon goniometer and the EIGER X 16M detector, and development of new data collection methods.

scholarly journals Determination of X-ray flux using silicon pin diodes

2009 ◽  
Vol 16 (2) ◽  
pp. 143-151 ◽  
Author(s):  
Robin L. Owen ◽  
James M. Holton ◽  
Clemens Schulze-Briese ◽  
Elspeth F. Garman
Keyword(s):  
Light Source ◽  
Energy Deposition ◽  
Photon Flux ◽  
Macromolecular Crystallography ◽  
Pin Diodes ◽  
Web Based ◽  
X Ray ◽  
Swiss Light Source ◽  
Flux Incident

Accurate measurement of photon flux from an X-ray source, a parameter required to calculate the dose absorbed by the sample, is not yet routinely available at macromolecular crystallography beamlines. The development of a model for determining the photon flux incident on pin diodes is described here, and has been tested on the macromolecular crystallography beamlines at both the Swiss Light Source, Villigen, Switzerland, and the Advanced Light Source, Berkeley, USA, at energies between 4 and 18 keV. These experiments have shown that a simple model based on energy deposition in silicon is sufficient for determining the flux incident on high-quality silicon pin diodes. The derivation and validation of this model is presented, and a web-based tool for the use of the macromolecular crystallography and wider synchrotron community is introduced.

scholarly journals Automatic loop centring with a high-precision goniometer head at the SLS macromolecular crystallography beamlines

2011 ◽  
Vol 18 (4) ◽  
pp. 595-600 ◽  
Author(s):  
Anuschka Pauluhn ◽  
Claude Pradervand ◽  
Daniel Rossetti ◽  
Marco Salathe ◽  
Clemens Schulze-Briese
Keyword(s):  
Data Collection ◽  
Light Source ◽  
High Precision ◽  
Initial Position ◽  
Macromolecular Crystallography ◽  
Centre Of Mass ◽  
Mass Detection ◽  
Swiss Light Source ◽  
Set Up ◽  
Goniometer Head

Automatic loop centring has been developed as part of the automation process in crystallographic data collection at the Swiss Light Source. The procedure described here consists of an optional set-up part, in which the background images are taken, and the actual centring part. The algorithm uses boundary and centre-of-mass detection at two different microscope image magnifications. Micromounts can be handled as well. Centring of the loops can be achieved in 15–26 s, depending on their initial position, and as fast as manual centring. The alignment of the sample is carried out by means of a new flexural-hinge-based compact goniometer head. The device features an electromagnet for robotic wet mounting of samples. The circle of confusion was measured to be smaller than 1 µm (r.m.s.); its bidirectional backlash is below 2 µm.

On Axis Resonance Raman Microspectroscopy Combined with Macromolecular Crystallography at the Swiss Light Source

2010 ◽  
Author(s):  
Martin R. Fuchs ◽  
Guillaume Pompidor ◽  
Vincent Thominet ◽  
Robin L. Owen ◽  
Arwen Pearson ◽  
...  
Keyword(s):  
Light Source ◽  
Resonance Raman ◽  
Raman Microspectroscopy ◽  
Macromolecular Crystallography ◽  
Swiss Light Source

scholarly journals Data acquisition and analysis software at the Swiss Light Source macromolecular crystallography beamlines

2016 ◽  
Vol 72 (a1) ◽  
pp. s192-s192
Author(s):  
Justyna A. Wojdyla ◽  
Ezequiel Panepucci ◽  
Jakub Kaminski ◽  
Simon Ebner ◽  
Xiaoqiang Wang ◽  
...  
Keyword(s):  
Data Acquisition ◽  
Light Source ◽  
Macromolecular Crystallography ◽  
Analysis Software ◽  
Swiss Light Source

scholarly journals Design of a multipurpose sample cell holder for the Diamond Light Source high-throughput SAXS beamline B21

2021 ◽  
Vol 28 (1) ◽  
pp. 318-321
Author(s):  
Charlotte Jennifer Chante Edwards-Gayle ◽  
Nikul Khunti ◽  
Ian W. Hamley ◽  
Katsuaki Inoue ◽  
Nathan Cowieson ◽  
...  
Keyword(s):  
3D Printing ◽  
Light Source ◽  
High Throughput ◽  
Low Cost ◽  
Sample Cell ◽  
Sample Changer

The design of a multipurpose sample cell holder for the high-throughput (HT) beamline B21 is presented. The device is compatible with the robot bioSAXS sample changer currently installed on BM29, ESRF, and P12 Petra IV synchrotrons. This work presents an approach that uses 3D-printing to make hardware alterations which can expand the versatility of HT beamlines at low cost.

scholarly journals A new on-axis micro-spectrophotometer for combining Raman, fluorescence and UV/Vis absorption spectroscopy with macromolecular crystallography at the Swiss Light Source

2013 ◽  
Vol 20 (5) ◽  
pp. 765-776 ◽  
Author(s):  
Guillaume Pompidor ◽  
Florian S. N. Dworkowski ◽  
Vincent Thominet ◽  
Clemens Schulze-Briese ◽  
Martin R. Fuchs
Keyword(s):  
Light Source ◽  
Detection Efficiency ◽  
Resonance Raman Spectroscopy ◽  
Optical Methods ◽  
Macromolecular Crystallography ◽  
X Ray Diffraction ◽  
X Ray ◽  
Application Range ◽  
Swiss Light Source ◽  
And Control

The combination of X-ray diffraction experiments with optical methods such as Raman, UV/Vis absorption and fluorescence spectroscopy greatly enhances and complements the specificity of the obtained information. The upgraded version of thein situon-axis micro-spectrophotometer, MS2, at the macromolecular crystallography beamline X10SA of the Swiss Light Source is presented. The instrument newly supports Raman and resonance Raman spectroscopy, in addition to the previously available UV/Vis absorption and fluorescence modes. With the recent upgrades of the spectral bandwidth, instrument stability, detection efficiency and control software, the application range of the instrument and its ease of operation were greatly improved. Its on-axis geometry with collinear X-ray and optical axes to ensure optimal control of the overlap of sample volumes probed by each technique is still unique amongst comparable facilities worldwide and the instrument has now been in general user operation for over two years.

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