scholarly journals Structure of in vivo protein crystals from viviparous Diploptera punctata

2017 ◽  
Vol 73 (a2) ◽  
pp. C177-C177
Author(s):  
Sanchari Banerjee ◽  
Nathan P. Coussens ◽  
François-Xavier Gallat ◽  
Nitish Sathyanarayanan ◽  
Koichiro J. Yagi ◽  
...  
Keyword(s):  
Protein Crystals ◽  
Diploptera Punctata

Sectioned rubisco crystals in TEM

1990 ◽  
Vol 48 (3) ◽  
pp. 664-665
Author(s):  
Gunnel Karlsson ◽  
Jan-Olov Bovin ◽  
Michael Bosma
Keyword(s):  
Plant Cell ◽  
Carbon Fixation ◽  
Unit Cell ◽  
Protein Crystals ◽  
Unit Cell Parameters ◽  
Cell Parameters ◽  
Photosynthetic Carbon Fixation ◽  
Photosynthetic Carbon

RuBisCO (D-ribulose-l,5-biphosphate carboxylase/oxygenase) is the most aboundant enzyme in the plant cell and it catalyses the key carboxylation reaction of photosynthetic carbon fixation, but also the competing oxygenase reaction of photorespiation. In vitro crystallized RuBisCO has been studied earlier but this investigation concerns in vivo existance of RuBisCO crystals in anthers and leaves ofsugarbeets. For the identification of in vivo protein crystals it is important to be able to determinethe unit cell of cytochemically identified crystals in the same image. In order to obtain the best combination of optimal contrast and resolution we have studied different staining and electron accelerating voltages. It is known that embedding and sectioning can cause deformation and obscure the unit cell parameters.

Allatostatins in the haemolymph of Diploptera punctata and their effect in vivo

1993 ◽  
Vol 39 (12) ◽  
pp. 1001-1005 ◽  
Author(s):  
Andrea P. Woodhead ◽  
Wendy Y. Asano ◽  
Barbara Stay
Keyword(s):  
Diploptera Punctata

Design of a CO-releasing Extracellular Scaffold Using in Vivo Protein Crystals

2015 ◽  
Vol 44 (3) ◽  
pp. 342-344 ◽  
Author(s):  
Hiroyasu Tabe ◽  
Takuya Shimoi ◽  
Kenta Fujita ◽  
Satoshi Abe ◽  
Hiroshi Ijiri ◽  
...  
Keyword(s):  
Protein Crystals ◽  
Extracellular Scaffold

The possible role of juvenile hormone esterase in the regulation of juvenile hormone titre in the female cockroach Diploptera punctata

1983 ◽  
Vol 61 (7) ◽  
pp. 811-817 ◽  
Author(s):  
Daniela Rotin ◽  
Stephen S. Tobe
Keyword(s):  
Juvenile Hormone ◽  
Diploptera Punctata ◽  
Juvenile Hormone Esterase ◽  
Adult Females ◽  
Acetate Esterase ◽  
Triton X ◽  
Naphthyl Acetate

Juvenile hormone (JH) degradation in vitro and in vivo was studied in the viviparous cockroach Diploptera punctata. In vitro studies with juvenile hormone esterase (JHE) and "general" or 1-naphthyl acetate esterase (NAcE) revealed that diethyl-p-nitrophenylphosphate (paraoxon) inhibited both JHE and NAcE activity, but the latter was more sensitive and was completely inhibited at 0.1 mM. NAcE activity was resistant to inhibition with Triton X-100, whereas JHE activity in haemolymph of adult females was inhibited 100% at Triton X-100 concentration of 0.25%. Eighty percent inhibition of JHE activity in vivo was observed following injection of 0.2 μL Triton X-100. In contrast to the previously observed dose-dependant increase in JHE activity, NAcE activity did not increase following treatment of allatectomized females with the JH analogue ethyl-2E,4E-3,7,11-trimethyl-2,4-dodecadienoate (hydroprene). Hydroprene was not catabolized in haemolymph of D. punctata in vitro. The half-life of C16 JH (JH III) in the haemolymph, in vivo, was 1.65 h for day 5 females and 2 h for day 6 females.

HAEMOCYTES OF THE PACIFIC BEETLE COCKROACH, DIPLOPTERA PUNCTATA

1970 ◽  
Vol 102 (7) ◽  
pp. 830-835 ◽  
Author(s):  
J. W. Arnold
Keyword(s):  
The Other ◽  
Diploptera Punctata ◽  
Proteinaceous Material ◽  
The Pacific ◽  
Morphological Specialization

AbstractHaemocytes of the Pacific beetle cockroach, Diploptera punctata (Esch.), are described and figured as they appear in vivo and in stained blood films. Although the haemocyte complex in this species is comprised of the same main categories as found commonly in other cockroaches, it is somewhat unusual due to morphological specialization among one category, the granular haemocytes. Most of the cells in this category are similar to those in other species, but some contain distinctive vesicles of mainly proteinaceous material, some are ovoid and contain spherule-size granules, and some are large, fusiform cells that are actively amoeboid in vivo. The other main categories, prohaemocytes, plasmatocytes, and spherule cells are fairly typical.

scholarly journals Design of a confined environment using protein cages and crystals for the development of biohybrid materials

2016 ◽  
Vol 52 (39) ◽  
pp. 6496-6512 ◽  
Author(s):  
Satoshi Abe ◽  
Basudev Maity ◽  
Takafumi Ueno
Keyword(s):  
Functional Properties ◽  
Protein Crystals ◽  
Feature Article ◽  
Protein Cages ◽  
Confined Environment

This feature article reviews the recent development of protein cages and in vivo and in vitro engineering of protein crystals with functional properties.

Fine Structural Changes in the Microvasculature of the Mouse Pinna: Aging and Radiation Injury

1974 ◽  
Vol 32 ◽  
pp. 54-55
Author(s):  
S. Phyllis Steamer ◽  
Rosemarie L. Devine
Keyword(s):  
Structural Changes ◽  
Radiation Treatment ◽  
Arterial Stenosis ◽  
Ultrastructural Changes ◽  
Vascular Effects ◽  
Microvascular Changes ◽  
Surrounding Connective Tissue ◽  
Fission Neutrons ◽  
Total Body

The importance of radiation damage to the skin and its vasculature was recognized by the early radiologists. In more recent studies, vascular effects were shown to involve the endothelium as well as the surrounding connective tissue. Microvascular changes in the mouse pinna were studied in vivo and recorded photographically over a period of 12-18 months. Radiation treatment at 110 days of age was total body exposure to either 240 rad fission neutrons or 855 rad 60Co gamma rays. After in vivo observations in control and irradiated mice, animals were sacrificed for examination of changes in vascular fine structure. Vessels were selected from regions of specific interest that had been identified on photomicrographs. Prominent ultrastructural changes can be attributed to aging as well as to radiation treatment. Of principal concern were determinations of ultrastructural changes associated with venous dilatations, segmental arterial stenosis and tortuosities of both veins and arteries, effects that had been identified on the basis of light microscopic observations. Tortuosities and irregularly dilated vein segments were related to both aging and radiation changes but arterial stenosis was observed only in irradiated animals.

Induction and Differentiation of Dental Epithelium in Vivo and in Vitro

1982 ◽  
Vol 40 ◽  
pp. 142-145
Author(s):  
E. J. Kollar
Keyword(s):  
Connective Tissue ◽  
Oral Mucosa ◽  
Basal Lamina ◽  
Functional Derivatives ◽  
Specific Source ◽  
Dental Epithelium ◽  
Connective Tissue Stroma

The differentiation and maintenance of many specialized epithelial structures are dependent on the underlying connective tissue stroma and on an intact basal lamina. These requirements are especially stringent in the development and maintenance of the skin and oral mucosa. The keratinization patterns of thin or thick cornified layers as well as the appearance of specialized functional derivatives such as hair and teeth can be correlated with the specific source of stroma which supports these differentiated expressions.

Ultrastructural Correlations between Clonal Human Tumor Colonies and in Vivo Neoplasms

1982 ◽  
Vol 40 ◽  
pp. 210-211
Author(s):  
M.J. Murphy ◽  
R.R. Price ◽  
J.C. Sloman
Keyword(s):  
Cultured Cells ◽  
Human Tumor ◽  
Cell Type ◽  
Tumor Mass ◽  
Initial Cell ◽  
Cloning Assay ◽  
Human Tumor Cloning ◽  
The Relationship

The in vitro human tumor cloning assay originally described by Salmon and Hamburger has been applied recently to the investigation of differential anti-tumor drug sensitivities over a broad range of human neoplasms. A major problem in the acceptance of this technique has been the question of the relationship between the cultured cells and the original patient tumor, i.e., whether the colonies that develop derive from the neoplasm or from some other cell type within the initial cell population. A study of the ultrastructural morphology of the cultured cells vs. patient tumor has therefore been undertaken to resolve this question. Direct correlation was assured by division of a common tumor mass at surgical resection, one biopsy being fixed for TEM studies, the second being rapidly transported to the laboratory for culture.

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