Structures of designed armadillo-repeat proteins show propagation of inter-repeat interface effects

The armadillo repeat serves as a scaffold for the development of modular peptide-recognition modules. In order to develop such a system, three crystal structures of designed armadillo-repeat proteins with third-generation N-caps (YIII-type), four or five internal repeats (M-type) and second-generation C-caps (AII-type) were determined at 1.8 Å (His-YIIIM4AII), 2.0 Å (His-YIIIM5AII) and 1.95 Å (YIIIM5AII) resolution and compared with those of variants with third-generation C-caps. All constructs are full consensus designs in which the internal repeats have exactly the same sequence, and hence identical conformations of the internal repeats are expected. The N-cap and internal repeats M1to M3are indeed extremely similar, but the comparison reveals structural differences in internal repeats M4and M5and the C-cap. These differences are caused by long-range effects of the C-cap, contacting molecules in the crystal, and the intrinsic design of the repeat. Unfortunately, the rigid-body movement of the C-terminal part impairs the regular arrangement of internal repeats that forms the putative peptide-binding site. The second-generation C-cap improves the packing of buried residues and thereby the stability of the protein. These considerations are useful for future improvements of an armadillo-repeat-based peptide-recognition system.

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Image Recognition Based on Euler Sequence by PCNN

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.228-229.1174 ◽

2011 ◽

Vol 228-229 ◽

pp. 1174-1180

Author(s):

Shui Ming He ◽

Yan Bin Ning ◽

Lei Fu ◽

Jian Ying Qin

Keyword(s):

Image Recognition ◽

Euler Number ◽

Recognition System ◽

Time Sequence ◽

Third Generation ◽

Sequence Entropy ◽

The Third ◽

Number Sequences ◽

Generation Pulse ◽

The Stability

The aim of this paper is to research the image recognition system and its applications, which is based on combining the traditional BP neural networks and the third-generation pulse coupled neural network (PCNN).The process is by extracting the image's Euler number sequences, then through the fast Fourier transform, and finally as inputs of the pattern classification. In order to test the stability of the system, we make some varies by rotating, tension and compressing the original image, meanwhile, combined with extracting the features such as time sequence, entropy sequence to do compare, the recognition results is satisfactory.

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Computationally Designed Armadillo Repeat Proteins for Modular Peptide Recognition

Journal of Molecular Biology ◽

10.1016/j.jmb.2016.09.012 ◽

2016 ◽

Vol 428 (22) ◽

pp. 4467-4489 ◽

Cited By ~ 9

Author(s):

Christian Reichen ◽

Simon Hansen ◽

Cristina Forzani ◽

Annemarie Honegger ◽

Sarel J. Fleishman ◽

...

Keyword(s):

Peptide Recognition ◽

Repeat Proteins ◽

Armadillo Repeat

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A New Method of Parameters Determined in PCNN

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.548-549.1059 ◽

2014 ◽

Vol 548-549 ◽

pp. 1059-1063 ◽

Cited By ~ 1

Author(s):

Shui Ming He ◽

Dian Hong Wang

Keyword(s):

Neural Network ◽

Neural Networks ◽

Fourier Transform ◽

Recognition System ◽

Third Generation ◽

Original Image ◽

The Third ◽

Entropy Information ◽

Generation Pulse ◽

The Stability

The aim of this paper is to research the image recognition system and its applications, which is based on combining the traditional BP neural networks and the third-generation pulse coupled neural network (PCNN).The process is by extracting the image's time and entropy sequences, then through the fast Fourier transform, and finally as inputs of the pattern classification. In order to test the stability of the system, we make some varies by rotating, tension and compressing the original image, meanwhile, combined with the statistics of the entropy information to determine the time delay parameter of the pcnn, the recognition results is satisfactory.

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Feasibility of Using Floor Vibration to Detect Human Falls

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18010200 ◽

2020 ◽

Vol 18 (1) ◽

pp. 200

Author(s):

Yu Shao ◽

Xinyue Wang ◽

Wenjie Song ◽

Sobia Ilyas ◽

Haibo Guo ◽

...

Keyword(s):

Machine Learning ◽

Human Body ◽

Nearest Neighbor ◽

Body Movement ◽

Modern Society ◽

Recognition System ◽

Machine Learning Algorithms ◽

K Nearest Neighbor ◽

Study Results ◽

Floor Vibration

With the increasing aging population in modern society, falls as well as fall-induced injuries in elderly people become one of the major public health problems. This study proposes a classification framework that uses floor vibrations to detect fall events as well as distinguish different fall postures. A scaled 3D-printed model with twelve fully adjustable joints that can simulate human body movement was built to generate human fall data. The mass proportion of a human body takes was carefully studied and was reflected in the model. Object drops, human falling tests were carried out and the vibration signature generated in the floor was recorded for analyses. Machine learning algorithms including K-means algorithm and K nearest neighbor algorithm were introduced in the classification process. Three classifiers (human walking versus human fall, human fall versus object drop, human falls from different postures) were developed in this study. Results showed that the three proposed classifiers can achieve the accuracy of 100, 85, and 91%. This paper developed a framework of using floor vibration to build the pattern recognition system in detecting human falls based on a machine learning approach.

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StaRProtein, A Web Server for Prediction of the Stability of Repeat Proteins

PLoS ONE ◽

10.1371/journal.pone.0119417 ◽

2015 ◽

Vol 10 (3) ◽

pp. e0119417

Author(s):

Yongtao Xu ◽

Xu Zhou ◽

Meilan Huang

Keyword(s):

Web Server ◽

Repeat Proteins ◽

The Stability

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Sa1.4 Do third generation proximal femur nails have better outcome than second generation nails

Injury ◽

10.1016/s0020-1383(13)70071-8 ◽

2013 ◽

Vol 44 ◽

pp. S16

Author(s):

R. Armagan ◽

T. Isik ◽

M. Kanar ◽

H.B. Sezer ◽

O.T. Eren

Keyword(s):

Proximal Femur ◽

Second Generation ◽

Third Generation

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The Brown Mite, Bryobia arborea Morgan and Anderson (Acarina: Tetranychidae), on Apple in Nova Scotia.: I. Influence of Intratree Distribution on the Selection of Sampling Units. II. Differences in Habits and Seasonal Trends in Orchards With Bivoltine and Trivoltine Populations. III. Distribution of Diapause Eggs. IV. Hatching of Diapause Eggs

The Canadian Entomologist ◽

10.4039/ent971303-12 ◽

1965 ◽

Vol 97 (12) ◽

pp. 1303-1318 ◽

Cited By ~ 6

Author(s):

H. J. Herbert

Keyword(s):

Nova Scotia ◽

First Generation ◽

Second Generation ◽

Central Area ◽

Third Generation ◽

Apple Trees ◽

Seasonal Trends ◽

Sample Unit ◽

The Third ◽

Selection Of

AbstractIn Nova Scotia one leaf cluster with an adjoining 1 inch of twig taken from the inside of each of 10 apple trees replicated four times is an adequate sample unit to measure the density of the brown mite.The brown mite has one generation with a partial second in some orchards and one with a partial second and partial third in others. The first generation adults in the bivoltine and trivoltine populations lay summer eggs on the leaves and twigs, and diapause eggs on tin twigs. The second generation adults in the bivoltine populations lay only diapause eggs; in the trivoltine populations they lay both summer and diapause eggs. The adults of the third generation lay only diapause eggs.The brown mite is found on both the leaves and woody parts of the tree. In orchards with bivoltine populations the proportion of mites on leaves reached a peak of 80% by mid-July, but thereafter gradually decreased to 10% by the end of August. However, in orchards with trivoltine populations the proportion of mites on leaves reached a peak of 80 to 90% by mid-July, remained constant until mid-August, and thereafter decreased to approximately 40% by the end of August.The number of diapause eggs laid by adults of each generation in both the bivoltine and trivoltine populations varies widely. The eggs are deposited on the trunk as well as on the branches, with the heaviest deposition in the central area of the tree. The diapause eggs laid by adults of the first generation are the last to hatch and those laid by the third generation are the first to hatch the following spring.The factors responsible for the differences in the number of generations and in the number of diapause eggs laid are unknown.

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Mid-term Results of Hydroxyapatite-Coated Threaded Cup Implanted without Supplementary Supporting Screws

Hip International ◽

10.1177/112070000901900204 ◽

2009 ◽

Vol 19 (2) ◽

pp. 102-108 ◽

Cited By ~ 2

Author(s):

Stergios J. Trapotsis ◽

George E. Petsatodis ◽

Petros D. Antonarakos ◽

Panagiotis K. Givissis ◽

Anastasios G. Christodoulou ◽

...

Keyword(s):

Second Generation ◽

Bone Ingrowth ◽

Harris Hip Score ◽

Radiographic Examination ◽

Acetabular Cup ◽

Osteolytic Lesions ◽

The Stability ◽

Hip Arthroplasties ◽

Mid Term Results

We retrospectively reviewed the clinical, radiographic, and survivorship outcomes in a series of 180 patients that underwent 204 primary Total Hip Arthroplasties with the use of a second generation threaded hydroxyapatite-coated acetabular cup that was implanted without any supplementary supporting screws. At an average follow-up period of 10.2 (range: 8–14) years, one hundred and seventy-four patients (198 cups) were available for assessment. All patients underwent detailed physical and radiographic examination; their functional status was evaluated according to the Harris Hip Score (HHS). Following their digitization, all radiographs were further reviewed in order to determine the existence of any migration of the acetabular cup. Osteolytic lesions, radiolucent lines or zones of increased bone density were also recorded and classified according to the system of DeLee & Charnley. The modified Engh's criteria were used in order to evaluate the stability of the prosthesis. The patients' mean HHS at their latest follow-up visit (97.24 points) was statistically significantly better than the preoperative mean score of 40.31 points (p<0.001). Radiographic analysis showed “stable with bone ingrowth” fixation (modified Engh's criteria) of all implants with no significant migration of the cup (mean cranial migration: 0.597 mm, mean horizontal migration: 0.607 mm, mean observed difference of the cup's inclination angle: 0.26 degrees). No areas of significant osteolysis were found. The cumulative survival rate of the implants was 97.05%. Our results suggest that second generation hydroxyapatite-coated threaded acetabular cups can be successfully implanted (and achieve excellent results) without the use of any supplementary supporting screws.

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