AbstractEstimation of turbulence intensity with a fast-response thermistor is examined by comparing the energy dissipation rate from a Fastip Probe, model 07 (FP07), thermistor with from a shear probe, both of which are attached to a free-fall microstructure profiler with the fall rate of 0.6–0.7 m s−1. Temperature gradient spectra corrected with previously introduced frequency response functions represented by a single-pole low-pass filter yields with a bias that strongly depends on turbulence intensity. Meanwhile, the correction with the form of a double-pole low-pass filter derives less bias than of single-pole low-pass filter. The rate is compatible with when the double-pole correction with the time constant of 3 × 10−3 s is applied, and 68% of data are within a factor of 2.8 of in the wide range of = 10−10–3 × 10−7 W kg−1. The rate is still compatible with even in the anisotropy range, where the buoyancy Reynolds number is 20–100. Turbulence estimation from the fast-response thermistor is thus confirmed to be valid in this range by applying the appropriate correction to temperature gradient spectra. Measurements with fast-response thermistors, which have not been common because of their poor frequency response, are less sensitive to the vibration of profilers than those with shear probes. Hence, measurements could be available when a fast-response thermistor is attached to a CTD frame or a float, which extends the possibility of obtaining much more turbulence data in deep and wide oceans.