Using Magnetic Nanoparticles to Enhance Site-specific Gene Transfection on Magneto-Electroporation Microchips

2007 ◽  
Author(s):  
Ming-Kai Liu ◽  
Hung-Yi Chen ◽  
Yi-Lung Wang ◽  
Shen-Shing Tsai ◽  
Min Li ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Gene Transfection ◽  
Specific Gene ◽  
Site Specific

scholarly journals Site-specific gene expression analysis using an automated tissue micro-dissection punching system

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Takuya Yoda ◽  
Masahito Hosokawa ◽  
Kiyofumi Takahashi ◽  
Chikako Sakanashi ◽  
Haruko Takeyama ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Specific Gene ◽  
Site Specific ◽  
Specific Gene Expression

Calcium ionophore A23187 induces expression of glucose-regulated genes and their heterologous fusion genes

1985 ◽  
Vol 5 (6) ◽  
pp. 1212-1219
Author(s):  
E Resendez ◽  
J W Attenello ◽  
A Grafsky ◽  
C S Chang ◽  
A S Lee
Keyword(s):  
Gene Transfection ◽  
Calcium Ionophore ◽  
Fold Increase ◽  
Calcium Ionophore A23187 ◽  
Induced Response ◽  
Specific Gene ◽  
Regulatory Sequence ◽  
Cdna Clones ◽  
Ionophore A23187 ◽  
Flanking Sequence

Using two cDNA clones which encode hamster genes specifically induced by glucose starvation, we demonstrated that an 8- and 30-fold increase, respectively, in the transcription rates of these genes was coordinately effected by calcium ionophore A23187 treatment, resulting in a similar increase in the steady-state levels of their mRNAs. This response was observed within several hours of ionophore treatment in several mammalian cell types and appeared to be specifically mediated by A23187 but not by other ionophores in general. To define the regulatory sequence which mediates this Ca2+-induced response, we showed by gene transfection techniques that the 5' flanking sequence of a rat glucose-regulated gene contained the region for induction by A23187. The system reported here offers attractive features for the study of specific gene regulation by Ca2+.

Site-specific, covalent immobilization of an engineered enterokinase onto magnetic nanoparticles through transglutaminase-catalyzed bioconjugation

2019 ◽  
Vol 177 ◽  
pp. 506-511 ◽  
Author(s):  
Jing-Hong Wang ◽  
Ming-Ze Tang ◽  
Xiao-Tian Yu ◽  
Chong-Mei Xu ◽  
Hong-Ming Yang ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Covalent Immobilization ◽  
Site Specific

scholarly journals Generation of a nicking enzyme that stimulates site-specific gene conversion from the I-AniI LAGLIDADG homing endonuclease

2009 ◽  
Vol 106 (13) ◽  
pp. 5099-5104 ◽  
Author(s):  
A. McConnell Smith ◽  
R. Takeuchi ◽  
S. Pellenz ◽  
L. Davis ◽  
N. Maizels ◽  
...  
Keyword(s):  
Gene Conversion ◽  
Homing Endonuclease ◽  
Specific Gene ◽  
Site Specific ◽  
Nicking Enzyme

Laser microdissection as a new tool to investigate site-specific gene expression in enteric ganglia of the human intestine

2010 ◽  
Vol 22 (2) ◽  
pp. 168-e52 ◽  
Author(s):  
m. böttner ◽  
f. bär ◽  
h. von koschitzky ◽  
k. tafazzoli ◽  
u. j. roblick ◽  
...  
Keyword(s):  
Gene Expression ◽  
Laser Microdissection ◽  
Specific Gene ◽  
Human Intestine ◽  
Site Specific ◽  
Specific Gene Expression ◽  
Enteric Ganglia
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