scholarly journals Atomic force microscopy-based single-cell indentation: Experimentation and finite element simulation

2009 ◽  
Author(s):  
Hamid Ladjal ◽  
Jean-Luc Hanus ◽  
Anand Pillarisetti ◽  
Carol Keefer ◽  
Antoine Ferreira ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Finite Element ◽  
Single Cell ◽  
Finite Element Simulation ◽  
Force Microscopy ◽  
Atomic Force ◽  
Element Simulation

Biomechanical Heterogeneity of Living Cells: Comparison between Atomic Force Microscopy and Finite Element Simulation

Langmuir ◽  
2018 ◽  
Vol 35 (23) ◽  
pp. 7578-7587 ◽  
Author(s):  
Guanlin Tang ◽  
Massimiliano Galluzzi ◽  
Bokai Zhang ◽  
Yu-Lin Shen ◽  
Florian J. Stadler
Keyword(s):  
Atomic Force Microscopy ◽  
Finite Element ◽  
Finite Element Simulation ◽  
Living Cells ◽  
Force Microscopy ◽  
Atomic Force ◽  
Element Simulation

scholarly journals A multi-structural finite element model to simulate atomic force microscopy nanoindentation of single cells

2019 ◽  
Author(s):  
Stefania Marcotti ◽  
Gwendolen C Reilly ◽  
Damien Lacroix
Keyword(s):  
Atomic Force Microscopy ◽  
Finite Element ◽  
Single Cell ◽  
Cell Nucleus ◽  
Single Cells ◽  
Element Model ◽  
Cell Stiffness ◽  
Force Microscopy ◽  
Atomic Force ◽  
Experimental Findings

AbstractSingle cell mechanical properties represent an increasingly studied descriptor for health and disease. Atomic force microscopy (AFM) has been widely used to measure single cell stiffness, despite its experimental limitations. The development of a computational framework to simulate AFM nanoindentation experiments could be a valuable tool to complement experimental findings. A single cell multi-structural finite element model was designed to this aim by using confocal images of bone cells, comprised of the cell nucleus, cytoplasm and actin cytoskeleton. The computational cell stiffness values were in the range of experimental values acquired on the same cells for nanoindentation of the cell nucleus and periphery, despite showing higher stiffness for the nucleus than for the periphery, oppositely to the average experimental findings. These results suggest it would be of interest to model different single cells with known experimental effective moduli to evaluate the ability of the computational models to replicate experimental results.

Visualization and Quantification of MicroRNA in a Single Cell Using Atomic Force Microscopy

2016 ◽  
Vol 138 (36) ◽  
pp. 11664-11671 ◽  
Author(s):  
Hyunseo Koo ◽  
Ikbum Park ◽  
Yoonhee Lee ◽  
Hyun Jin Kim ◽  
Jung Hoon Jung ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Single Cell ◽  
Force Microscopy ◽  
Atomic Force

Living cell study at the single-molecule and single-cell levels by atomic force microscopy

Nanomedicine ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. 1625-1637 ◽  
Author(s):  
Xiaoli Shi ◽  
Xuejie Zhang ◽  
Tie Xia ◽  
Xiaohong Fang
Keyword(s):  
Atomic Force Microscopy ◽  
Single Cell ◽  
Single Molecule ◽  
Living Cell ◽  
Force Microscopy ◽  
Atomic Force ◽  
Cell Study
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