Micro Reverse Transcription Polymerase Chain Reaction Systems Using Super-paramagnetic Beads for Virus Detection

2006 ◽  
Author(s):  
Kang-Yi Lien ◽  
Wan-Chi Lee ◽  
Huan-Yao Lei ◽  
Gwo-Bin Lee
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Virus Detection ◽  
Chain Reaction ◽  
Reaction Systems ◽  
Polymerase Chain ◽  
Paramagnetic Beads

scholarly journals Reverse transcription-polymerase chain reaction assay for rabies virus detection

2004 ◽  
Vol 56 (3) ◽  
pp. 398-400 ◽  
Author(s):  
J.V. Dantas Junior ◽  
L.M.S. Kimura ◽  
M.S.R. Ferreira ◽  
A.M. Fialho ◽  
M.M.S. Almeida ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Rabies Virus ◽  
Reverse Transcription ◽  
Polymerase Chain Reaction Assay ◽  
Virus Detection ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Dengue virus detection by using reverse transcription-polymerase chain reaction in saliva and progeny of experimentally infected Aedes albopictus from Brazil

2004 ◽  
Vol 99 (8) ◽  
pp. 809-814 ◽  
Author(s):  
Márcia Gonçalves de Castro ◽  
Rita Maria Ribeiro Nogueira ◽  
Hermann Gonçalves Schatzmayr ◽  
Marize Pereira Miagostovich ◽  
Ricardo Lourenço-de-Oliveira
Keyword(s):  
Polymerase Chain Reaction ◽  
Dengue Virus ◽  
Aedes Albopictus ◽  
Reverse Transcription ◽  
Virus Detection ◽  
Chain Reaction ◽  
Polymerase Chain

MULTIPLEX REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION FOR CHICKEN TUMOR VIRUS DETECTION

2015 ◽  
Vol 41 (04) ◽  
pp. 245-249
Author(s):  
Lih-Chiann Wang ◽  
Der-Yuh Lin ◽  
Wei Thong ◽  
Ching-Ho Wang
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Virus Detection ◽  
Economic Loss ◽  
Buffy Coat ◽  
Single Step ◽  
Chain Reaction ◽  
Tumor Diseases ◽  
Tumor Virus ◽  
Polymerase Chain

Tumor diseases occur frequently in chickens causing a great economic loss. Infected chickens’ pathological lesions are not pathognomonic. This study developed an accurate diagnosis for tumor diseases in chickens. Specific primers to reticuloendotheliosis virus (REV), avian leucosis virus subgroup A (ALV-A), avian leucosis virus subgroup J (ALV-J), and Marek’s disease virus (MDV) were combined into one tube with a single step multiplex reverse transcription polymerase chain reaction (mRT-PCR) performed to amplify the genes from each virus. A total of 117 sample pools containing blood and tissues were collected from chickens. Three of these pools (2.6%) showed REV positive, 22 (18.8%) ALV-J positive, 1 (0.8%) ALV-A positive, and 3 (2.6%) MDV positive. A total of 268 blood samples were used to compare the viral RNA detection from plasma using RT-PCR and provirus DNA from buffy coat using PCR. The result showed no difference from both tests. In conclusion, the present mRT-PCR could be used for tumor virus detections in chickens.

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