An Immunity/Susceptibility Test Method Using Electromagnetic Wave of Rotating Polarization

2004 ◽  
Vol 53 (4) ◽  
pp. 1184-1191 ◽  
Author(s):  
K. Murano ◽  
F. Xiao ◽  
Y. Kami
Keyword(s):  
Electromagnetic Wave ◽  
Test Method ◽  
Susceptibility Test

scholarly journals Evaluation of semisolid agar method for antifungal susceptibility test of T. rubrum

2016 ◽  
Vol 7 (1) ◽  
pp. 11
Author(s):  
Sultana Razia ◽  
Shahida Anwar ◽  
Md. Ruhul Amin Miah ◽  
Najmun Nahar ◽  
Ripon Barua
Keyword(s):  
Antifungal Agents ◽  
Antifungal Susceptibility ◽  
Antifungal Drugs ◽  
Test Method ◽  
Susceptibility Test ◽  
Broth Microdilution ◽  
Microdilution Method ◽  
Broth Microdilution Method ◽  
Antifungal Susceptibility Test ◽  
Mic Value

<p><strong>Background:</strong> With increasing fungal disease many newer antifungal drugs are available with different spectrum of activ­ity. Antifungal susceptibility test will help clinicians for selection of effective drug and thereby treatment of patient.<strong> </strong></p><p><strong>Objective:</strong> The study was undertaken to perform a simple screening drug susceptibility test of T. rnbrum by Semi Solid Agar Antifungal Susceptibility (SAAS) <strong></strong></p><p><strong>Method:</strong> Perfonnance of susceptibility method was assessed by comparing the MICs of three commonly prescribed antifungal agents namely- tluconazole (FCZ), itraconazole (ITZ) and terbinafine (TER) to the CLSI (Clinical and Laboratory Standard Institute) recommended M-38, a broth microdilution method. <strong></strong></p><p><strong>Results:</strong> In SAAS method, among twenty nine T. rubrum, twenty five (86.2%) were susceptible (MIC range 0.5-64 µg/ml) to Fluconazole (FCZ) and four (13.7%) were resistant (MIC value &gt;64 µg/ml). In broth microdilution method, among twenty nine T. rubrum, twenty six (89.6%) were susceptible (MIC range 0.3-64 µg/ml) to FCZ and three (10.3%) were resistant (MIC value &gt;64 µg/ml). In case of both ITZ and TER, all were susceptible (MIC range 0.3-64 µg/ml) to both methods. The SAAS method demonstrated the susceptibility pattern of T. rubrum against FCZ, ITZ and TER usually within 72 to 96 hours after organism isolation and results were concordance with the results of CLSI broth microdilution method. <strong></strong></p><p><strong>Conclusion:</strong> Though it is a newer method with proper standardization of the test method, SAAS method is simple and easily applicable screening method for susceptibility testing of antifungal agents against dermatophytes in any microbiology laboratories.</p>

scholarly journals Diagnostic Techniques and Antimicrobial Susceptibility Test of Helicobacter Pylori Infection in Gashua Town

2019 ◽  
Vol 15 (10) ◽  
pp. 126
Author(s):  
A.G. Dagona ◽  
I.G Dagona ◽  
K Muhammad ◽  
F.A. Garba ◽  
W.D Taura ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Likelihood Ratio ◽  
Antimicrobial Susceptibility ◽  
Accurate Diagnosis ◽  
Test Method ◽  
Susceptibility Test ◽  
Antimicrobial Susceptibility Test ◽  
Noninvasive Methods ◽  
Urease Test ◽  
H Pylori

<p class="0abstract">Helicobacter pylori eradication continues to be a challenge in a group of patients after the failure of several therapeutic regimen attempts. Treatment regimen is generally chosen on the basis of the prevalence of bacterial resistance detected against the tested antibiotics. 60 sixty  (60), adult out patients presented to Gastroenterology clinic of General Hospital Gashua undertook multiple gastric biopsy and specimens were carried for gram stain, culture, antimicrobial sensitivity testing, rapid urease test and histology. Antimicrobial susceptibility test were carryout using Epsilometer testing Kit (E-test) method and tested against Omeprazole, metronidazole Ranitidine, and Amoxicillin. About Sixty percent (30%) of the study population was positive for H. pylori infection (mean age of 34 years ± 15), 60% were males while 40% are women which are mostly married. Helicobacter pylori culture showed a sensitivity of 55% (95% CI (29.5– 62.1), specificity of 97% (95%CI (80.5–100%), positive likelihood ratio of 19.93 (95% CI (1.254– 317.04) and a negative likelihood ratio of 0.76 (95% CI (0.406–0.772). Eighteen (18) strains of Helicobacter pylori isolated 14 are sensitive to Amoxicillin, Ciprofloxacin, metronidazole; amoxicillin three (4) strains were resistant to ciprofloxacin and Amoxicillin and metronidazole when tested. It is imperative to have indebted knowledge on diagnostic method and antibiotic susceptibility patterns in the study area, since the accurate diagnosis of H. pylori is the idealistic view for both gastroenterologists and microbiologists, using synergistically invasive and noninvasive methods will be a future challenge in medical research topics. It is clear that recent advances in invasive and noninvasive methods for accurate diagnosis of the H. pylori can drastically change upcoming guidelines attributed with the management of this infection.</p>

New Turner-Fairbank Alkali-Silica Reaction Susceptibility Test for Aggregate Evaluation

2021 ◽  
pp. 036119812110045
Author(s):  
Jose F. Muñoz ◽  
Chandni Balachandran ◽  
Terence S. Arnold
Keyword(s):  
False Negative ◽  
Field Performance ◽  
Outdoor Exposure ◽  
Test Method ◽  
Susceptibility Test ◽  
Reactivity Index ◽  
Alkali Silica Reaction ◽  
River Sand ◽  
Negative Results ◽  
Alkali Silica Reactivity

The ASTM C1260 and ASTM C1293 are generally accepted as being the best available accelerated tests to evaluate the alkali-silica reactivity of aggregates used in concrete. Unfortunately, these tests have limitations, such as the significant amount of false-positive and false-negative results in ASTM C1260 and the alkali leaching in ASTM C1293, that reduce their accuracy. This paper introduces an alternative test method, the Turner-Fairbank alkali-silica reaction (ASR) susceptibility test (T-FAST) that overcomes traditional limitations of both ASTM standards. In the new test, the ASR was accelerated by exposing the aggregates to a 1 N NaOH solution, three different amounts of CaO, and two temperatures for 21 days. The reactivity index (RI), calculated based on the 21-day concentrations of aluminum, calcium, and silicon in liquid phase, was used to assess the alkali-silica reactivity of 24 well-known aggregates—17 coarse and 7 fine. The results agreed with the classification of the same based on ASTM C1293 and historic field performance available in the literature. The alkali levels at which the ASR reaction was triggered in a selection of aggregates were measured using the T-FAST experimental set up. The threshold alkali values obtained matched those previously reported using accelerated concrete expansion tests as well as with concrete blocks in outdoor exposure sites. The alkali threshold determined for a river sand from Arkansas helped to understand the unexpected ASR distress observed in the field for an aggregate traditionally categorized as nonreactive. This case is a good example of mismatch between the information obtained from accelerated-ASR standard tests and field performance.

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