scholarly journals Diagnostic Techniques and Antimicrobial Susceptibility Test of Helicobacter Pylori Infection in Gashua Town

2019 ◽  
Vol 15 (10) ◽  
pp. 126
Author(s):  
A.G. Dagona ◽  
I.G Dagona ◽  
K Muhammad ◽  
F.A. Garba ◽  
W.D Taura ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Likelihood Ratio ◽  
Antimicrobial Susceptibility ◽  
Accurate Diagnosis ◽  
Test Method ◽  
Susceptibility Test ◽  
Antimicrobial Susceptibility Test ◽  
Noninvasive Methods ◽  
Urease Test ◽  
H Pylori

<p class="0abstract">Helicobacter pylori eradication continues to be a challenge in a group of patients after the failure of several therapeutic regimen attempts. Treatment regimen is generally chosen on the basis of the prevalence of bacterial resistance detected against the tested antibiotics. 60 sixty  (60), adult out patients presented to Gastroenterology clinic of General Hospital Gashua undertook multiple gastric biopsy and specimens were carried for gram stain, culture, antimicrobial sensitivity testing, rapid urease test and histology. Antimicrobial susceptibility test were carryout using Epsilometer testing Kit (E-test) method and tested against Omeprazole, metronidazole Ranitidine, and Amoxicillin. About Sixty percent (30%) of the study population was positive for H. pylori infection (mean age of 34 years ± 15), 60% were males while 40% are women which are mostly married. Helicobacter pylori culture showed a sensitivity of 55% (95% CI (29.5– 62.1), specificity of 97% (95%CI (80.5–100%), positive likelihood ratio of 19.93 (95% CI (1.254– 317.04) and a negative likelihood ratio of 0.76 (95% CI (0.406–0.772). Eighteen (18) strains of Helicobacter pylori isolated 14 are sensitive to Amoxicillin, Ciprofloxacin, metronidazole; amoxicillin three (4) strains were resistant to ciprofloxacin and Amoxicillin and metronidazole when tested. It is imperative to have indebted knowledge on diagnostic method and antibiotic susceptibility patterns in the study area, since the accurate diagnosis of H. pylori is the idealistic view for both gastroenterologists and microbiologists, using synergistically invasive and noninvasive methods will be a future challenge in medical research topics. It is clear that recent advances in invasive and noninvasive methods for accurate diagnosis of the H. pylori can drastically change upcoming guidelines attributed with the management of this infection.</p>

scholarly journals Antibiotic Resistance and Genotypes of Helicobacter pylori Strains in Patients with Gastroduodenal Disease in Southeast Poland

2019 ◽  
Vol 8 (7) ◽  
pp. 1071 ◽  
Author(s):  
Izabela Korona-Glowniak ◽  
Halina Cichoz-Lach ◽  
Radoslaw Siwiec ◽  
Sylwia Andrzejczuk ◽  
Andrzej Glowniak ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Helicobacter Pylori ◽  
Test Method ◽  
Rapid Urease Test ◽  
Microbial Culture ◽  
Virulence Markers ◽  
Gastroduodenal Disease ◽  
Urease Test ◽  
Gastric Biopsies ◽  
H Pylori

The aim of this study was to investigate genetic diversity of Helicobacter pylori virulence markers to predict clinical outcome as well as to determine an antibiotic susceptibility of H. pylori strains in Poland. Gastric biopsies from 132 patients with gastrointestinal disorders were tested for presence of H. pylori with the use of rapid urease test, microbial culture, and polymerase chain reaction (PCR) detection. The genetic diversity of 62 H. pylori positive samples was evaluated by detection of cagA and PCR-typing of vacA and iceA virulence-associated genes. Most common H. pylori genotypes were cagA(+)vacAs1m2 (27.4%) and cagA(−)vacAs2m2 (24.2%). In logistic regression analysis, we recognized the subsequent significant associations: gastritis with ureC, i.e., H. pylori infection (p = 0.006), BMI index (p = 0.032); and negatively with iceA1 (p = 0.049) and peptic ulcer with cagA (p = 0.018). Thirty-five H. pylori strains were cultured and tested by E-test method showing that 49% of strains were resistant to at least one of the tested antibiotics. This is the first study that reports the high incidence and diversity of allelic combination of virulence genes in gastroduodenitis patients in Poland. Genotyping of H. pylori strains confirmed the involvement of cagA gene and vacAs1m1 genotype in development and severity of gastric disorder.

scholarly journals Validation of diagnostic tests and epidemiology of Helicobacter pylori infection in Bangladesh

2018 ◽  
Vol 12 (05) ◽  
pp. 305-312 ◽  
Author(s):  
Hafeza Aftab ◽  
Yoshio Yamaoka ◽  
Faruque Ahmed ◽  
Azad AK Khan ◽  
Phawinee Subsomwong ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Likelihood Ratio ◽  
Sensitivity And Specificity ◽  
Diagnostic Tests ◽  
Age Groups ◽  
Endoscopic Examination ◽  
Helicobacter Pylori Infection ◽  
Predictive Values ◽  
Urease Test ◽  
H Pylori

Introduction: Helicobacter pylori infection is associated with gastritis, peptic ulcer, and gastric cancer. We conducted a cross-sectional study to compare five diagnostic tests for H. pylori infection and studied the epidemiology of the infection in Bangladesh. Methodology: Bangladeshi patients with dyspeptic symptoms referred for endoscopic examination were enrolled in this study. Each patient underwent upper endoscopic examination and four gastric biopsy specimens were taken. We used 5 tests for the diagnosis of H. pylori; culture, histology confirmed by immunohistochemistry, rapid urease test (RUT), urinary and serological test. Demographic and environmental variables were collected. Results: A total of 133 patients participated in the study, 61 males and mean age 37.3 ± 12.3 years. We used the culture and/or histology results as the gold standard to estimate the sensitivity, specificity, positive and negative predictive values for the studied diagnostic tests. RUT, culture and histology had high sensitivity and specificity with moderate positive and negative likelihood ratio, whereas urine test and serology showed a good sensitivity and specificity but poor likelihood ratio. The overall prevalence of H. pylori among study subjects was 47% with no difference between gender and age groups. Conclusions: The invasive tests showed better performance than noninvasive tests among Bangladeshi population. The overall prevalence of H. pylori was less than the previously reported in the region with no difference among all age groups.

The CDS Antimicrobial Susceptibility Test Method: fulfilling a need

2014 ◽  
Vol 35 (1) ◽  
pp. 35
Author(s):  
Sydney Bell ◽  
Jeanette Pham ◽  
Peter Newton ◽  
Thanh Nguyen
Keyword(s):  
Antimicrobial Susceptibility ◽  
Test Method ◽  
Susceptibility Test ◽  
Antimicrobial Susceptibility Test

scholarly journals Comparison of Serum IgG Antibody Test with Gastric Biopsy for the Detection of Helicobacter Pylori Infection among Egyptian Children

2015 ◽  
Vol 3 (2) ◽  
pp. 303-306 ◽  
Author(s):  
Mones M. Abu Shady ◽  
Hanan A. Fathy ◽  
Alaa Ali ◽  
Essam M. Galal ◽  
Gihan A. Fathy ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Likelihood Ratio ◽  
Serological Test ◽  
Standard Test ◽  
Helicobacter Pylori Infection ◽  
Igg Antibody ◽  
Serum Igg ◽  
Egyptian Children ◽  
Urease Test ◽  
H Pylori

BACKGROUND: In developing countries, Helicobacter pylori (H. pylori) infection is mainly acquired during childhood and may be a predisposing factor for peptic ulcer or gastric cancer later in life. Noninvasive diagnostic tools are particularly useful in children for screening tests and epidemiological studies. Data on serologic testing of children are lacking. Accurate noninvasive tests for diagnosing Helicobacter pylori infection in children are strongly required.AIM: The aim of this study was to evaluate the performance of a serological test (serum IgG antibody for H. pylori) in Egyptian children with recurrent abdominal pain necessitating endoscopy.SUBJECTS AND METHODS: One hundred children, referred to the endoscopy unit at Mansoura University. Upper endoscopy was done for each with rapid urease test (RUT) and histological examination as the gold standard test for detection of H. pylori infection. Serum samples were collected for detecting IgG for H. pylori infection.RESULTS: The mean age of the subjects included in the study was 7.23 ± 1.94 year. Serological test (IgG to H. pylori) was positive in 60% of all cases. A highly significant association between the standard test and the serological test at a cutoff > 10 U/ml at p = 0.001 were detected for the diagnosis of H. pylori infection. The sensitivity, specificity, positive likelihood ratio, and negative likelihood ratio for the IgG antibody a cutoff > 10 U/ml, were 96.5%, 93%, 13.83, 0.038 respectively.CONCLUSION: Serum IgG antibody to H. pylori infection has a high diagnostic value and can be considered as a suitable and reliable noninvasive test for detection of H. pylori infection.

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