The Effect of Temperature and Additives on the Dielectric Behavior of Human Whole Blood, Its Different Components and Cell Suspensions

2022 ◽  
pp. 1-1
Author(s):  
Sushma Kumari ◽  
Sujoy Kumar Samanta
Keyword(s):  
Whole Blood ◽  
Dielectric Behavior ◽  
Cell Suspensions ◽  
Effect Of Temperature ◽  
Human Whole Blood

Isolation of Nucleated Cells from Whole Blood v1

2021 ◽  
Author(s):  
Steven B. Wells ◽  
Peter A. Szabo ◽  
Nora Lam
Keyword(s):  
Single Cell ◽  
Whole Blood ◽  
Myeloid Cells ◽  
Cell Suspensions ◽  
Dilution Factor ◽  
Human Whole Blood ◽  
Density Centrifugation ◽  
Defined Media

This protocol describes a method for the isolation of pan-lymphocytes and pan-myeloid cells from human whole blood. By providing defined media formulations, volumes at each step, and a defined dilution factor for density centrifugation, it yields consistent single-cell suspensions across samples.

Human whole-blood oxygen affinity: effect of temperature

1984 ◽  
Vol 57 (2) ◽  
pp. 429-434 ◽  
Author(s):  
A. Zwart ◽  
G. Kwant ◽  
B. Oeseburg ◽  
W. G. Zijlstra
Keyword(s):  
Whole Blood ◽  
Oxygen Affinity ◽  
Heat Evolution ◽  
Factor H ◽  
Effect Of Temperature ◽  
Temperature Changes ◽  
Human Whole Blood ◽  
Healthy Donors ◽  
Blood Oxygen Affinity ◽  
O2 Dissociation Curve

phe effect of temperature changes on human whole-blood O2 affinity was measured in the blood of six healthy donors over almost the entire O2 saturation (SO2) range (1–99%). The results showed that temperature has no influence on the shape of the O2 dissociation curve, implying that the temperature coefficient (delta log PO2/delta T) is independent of SO2. Simultaneous measurements of the total (proton) Haldane factor (delta[HbH]/[delta HbO2]) at the five temperatures under study (22, 27, 32, 37, and 42 degrees C) revealed that this factor depends on temperature. The liberation of protons from hemoglobin appeared to be linear with respect to changes in SO2. We therefore conclude that the (proton) Bohr factor (H+ factor) is dependent on temperature over the entire SO2 range in the same way as previously described for SO2 = 50%. The exothermic oxygenation reaction in whole blood was accompanied by a heat evolution (delta HO2) of 42.7 kJ/mol (monomeric) hemoglobin.

scholarly journals Monocyte CD163 and CD36 Expression in Human Whole Blood and Isolated Mononuclear Cell Samples: Influence of Different Anticoagulants

2006 ◽  
Vol 13 (6) ◽  
pp. 704-707 ◽  
Author(s):  
Marcin Moniuszko ◽  
Krzysztof Kowal ◽  
Malgorzata Rusak ◽  
Miroslawa Pietruczuk ◽  
Milena Dabrowska ◽  
...  
Keyword(s):  
Density Gradient ◽  
Mononuclear Cell ◽  
Whole Blood ◽  
Cell Isolation ◽  
Scavenger Receptors ◽  
Published Data ◽  
Healthy Individuals ◽  
Human Whole Blood ◽  
Cd36 Expression

ABSTRACT We investigated whether the choice of anticoagulant or the application of density gradient mononuclear cell isolation may account for conflicting published data regarding the levels of the scavenger receptors' expression in healthy individuals. We demonstrate that the detection of CD163, but not CD36, differs dramatically among the methods.

scholarly journals Isolation and characterization of circulating pro-vascular progenitor cell subsets from human whole blood samples

2021 ◽  
Vol 2 (1) ◽  
pp. 100311
Author(s):  
Daniella C. Terenzi ◽  
Ehab Bakbak ◽  
Justin Z. Trac ◽  
Mohammad Al-Omran ◽  
Adrian Quan ◽  
...  
Keyword(s):  
Whole Blood ◽  
Progenitor Cell ◽  
Blood Samples ◽  
Human Whole Blood ◽  
Isolation And Characterization ◽  
Whole Blood Samples

The distribution of BrKα/RbKα peak intensity ratio in human whole blood samples

1994 ◽  
Vol 42 (3) ◽  
pp. 231-241 ◽  
Author(s):  
C. Shenberg ◽  
S. Spiegel ◽  
S. Chaitchik ◽  
P. Jordan ◽  
M. Kitzis ◽  
...  
Keyword(s):  
Whole Blood ◽  
Intensity Ratio ◽  
Peak Intensity Ratio ◽  
Blood Samples ◽  
Human Whole Blood ◽  
Peak Intensity ◽  
Whole Blood Samples

The effect of combined inhibition of C5aR1 and CD14 on phagocytosis of E. coli in human whole blood

Immunobiology ◽  
2016 ◽  
Vol 221 (10) ◽  
pp. 1220
Author(s):  
Dorte Christiansen ◽  
Espen Waage Skjeflo ◽  
Anne Landsem ◽  
Monica Dammen Mathisen ◽  
Hilde Fure ◽  
...  
Keyword(s):  
Whole Blood ◽  
Human Whole Blood ◽  
E Coli
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