Isolation of Nucleated Cells from Whole Blood v1

2021 ◽  
Author(s):  
Steven B. Wells ◽  
Peter A. Szabo ◽  
Nora Lam
Keyword(s):  
Single Cell ◽  
Whole Blood ◽  
Myeloid Cells ◽  
Cell Suspensions ◽  
Dilution Factor ◽  
Human Whole Blood ◽  
Density Centrifugation ◽  
Defined Media

This protocol describes a method for the isolation of pan-lymphocytes and pan-myeloid cells from human whole blood. By providing defined media formulations, volumes at each step, and a defined dilution factor for density centrifugation, it yields consistent single-cell suspensions across samples.

Preparation of Single Cell Suspension from Human Lymph Node Tissue v1

2021 ◽  
Author(s):  
Steven B. Wells ◽  
Peter A. Szabo ◽  
Nora Lam ◽  
Maya M.L. Poon
Keyword(s):  
Lymph Node ◽  
Cell Suspension ◽  
Single Cell ◽  
Myeloid Cells ◽  
Cell Suspensions ◽  
Dilution Factor ◽  
Single Cell Suspension ◽  
Density Centrifugation ◽  
Defined Media ◽  
Human Lymph Node

This protocol describes a method for the isolation of pan-lymphocytes, pan-myeloid cells, and progenitors from human lymph node tissue. By providing defined media formulations, volumes at each step, and a defined dilution factor for density centrifugation, it yields consistent single-cell suspensions across samples.

Isolation of Nucleated Cells from Bone Marrow Aspirate v1

2021 ◽  
Author(s):  
Steven B. Wells ◽  
Peter A. Szabo ◽  
Nora Lam
Keyword(s):  
Bone Marrow ◽  
Single Cell ◽  
Bone Marrow Aspirate ◽  
Myeloid Cells ◽  
Cell Suspensions ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Dilution Factor ◽  
Density Centrifugation ◽  
Defined Media

This protocol describes a method for the isolation of pan-lymphocytes, pan-myeloid cells, and progenitors from human bone marrow aspirate. By providing defined media formulations, volumes at each step, and a defined dilution factor for density centrifugation, it yields consistent single-cell suspensions across samples.

Preparation of Single Cell Suspension from Human Spleen Tissue v1

2021 ◽  
Author(s):  
Steven B. Wells ◽  
Peter A. Szabo
Keyword(s):  
Cell Suspension ◽  
Single Cell ◽  
Myeloid Cells ◽  
Cell Suspensions ◽  
Dilution Factor ◽  
Single Cell Suspension ◽  
Human Spleen ◽  
Spleen Tissue ◽  
Density Centrifugation ◽  
Defined Media

This protocol describes a method for the isolation of pan-lymphocytes, pan-myeloid cells, and progenitors from human spleen tissue. By providing defined media formulations, volumes at each step, and a defined dilution factor for density centrifugation, it yields consistent single-cell suspensions across samples.

Preparation of Single Cell Suspensions of the Intra-Epithelial Layer and Lamina Propria from Human Intestinal Tissue v1

2021 ◽  
Author(s):  
Steven B. Wells ◽  
Pranay Dogra ◽  
Josh Gray ◽  
Peter A. Szabo ◽  
Daniel Caron ◽  
...  
Keyword(s):  
Single Cell ◽  
Lamina Propria ◽  
Intestinal Tract ◽  
Distal Colon ◽  
Cell Suspensions ◽  
Epithelial Layer ◽  
Dilution Factor ◽  
Human Gut ◽  
Intestinal Tissue ◽  
Defined Media

This protocol describes a method for the isolation of the immune cells, structural and epithelial cells, and progenitors from the epithelial layer and the lamina propria of human gut sections of about one gram of tissue. By providing defined media formulations, volumes at each step, and a defined dilution factor for density centrifugation, it yields consistent single-cell suspensions across samples. This protocol can be used for any section of the intestinal tract from duodenum to distal colon.

Preparation of a Single Cell Suspension from Bronchoalevolar Lavage v1

2021 ◽  
Author(s):  
Peter A. Szabo ◽  
Steven B. Wells ◽  
Basak Ural
Keyword(s):  
Epithelial Cells ◽  
Cell Suspension ◽  
Single Cell ◽  
Immune Cells ◽  
Human Lung ◽  
Lavage Fluid ◽  
Cell Suspensions ◽  
Dilution Factor ◽  
Single Cell Suspension ◽  
Defined Media

This protocol describes a method for the isolation of the immune cells, structural and epithelial cells, and progenitors from lavage fluid collected from human lung. By providing defined media formulations, volumes at each step, and a defined dilution factor for density centrifugation, it yields consistent single-cell suspensions across samples.

Preparation of Single Cell Suspension from Human Lung Tissue v1

2021 ◽  
Author(s):  
Steven B. Wells ◽  
Peter A. Szabo ◽  
Basak Ural ◽  
Maya M.L. Poon
Keyword(s):  
Epithelial Cells ◽  
Cell Suspension ◽  
Single Cell ◽  
Lung Tissue ◽  
Immune Cells ◽  
Human Lung ◽  
Dilution Factor ◽  
Single Cell Suspension ◽  
Human Lung Tissue ◽  
Defined Media

This protocol describes a method for the isolation of the immune cells, structural and epithelial cells, and progenitors from human lung sections of about two grams. By providing defined media formulations, volumes at each step, and a defined dilution factor for density centrifugation, it yields consistent single-cell suspensions across samples.

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