Numerical Investigation on Arc Behavior in Low-Voltage Arc Chamber Considering Turbulence Effect

2014 ◽  
Vol 42 (10) ◽  
pp. 2716-2717 ◽  
Author(s):  
Mingzhe Rong ◽  
Ruiguang Ma ◽  
Junxing Chen ◽  
Chao Hou ◽  
Yushu Sun
2009 ◽  
Vol 49 (2) ◽  
pp. 22904 ◽  
Author(s):  
C. Brdys ◽  
D. Cajal ◽  
J.-P. Toumazet ◽  
F. Gary ◽  
A. Laurent ◽  
...  

2010 ◽  
Vol 49 (2) ◽  
pp. 22910 ◽  
Author(s):  
A. Mutzke ◽  
T. Rüther ◽  
M. Lindmayer ◽  
M. Kurrat
Keyword(s):  

2009 ◽  
Vol 37 (1) ◽  
pp. 261-269 ◽  
Author(s):  
Qiang Ma ◽  
Mingzhe Rong ◽  
Anthony B. Murphy ◽  
Yi Wu ◽  
Tiejun Xu

Author(s):  
Marek Malecki ◽  
J. Victor Small ◽  
James Pawley

The relative roles of adhesion and locomotion in malignancy have yet to be clearly established. In a tumor, subpopulations of cells may be recognized according to their capacity to invade neighbouring tissue,or to enter the blood stream and metastasize. The mechanisms of adhesion and locomotion are themselves tightly linked to the cytoskeletal apparatus and cell surface topology, including expression of integrin receptors. In our studies on melanomas with Fluorescent Microscopy (FM) and Cell Sorter(FACS), we noticed that cells in cultures derived from metastases had more numerous actin bundles, then cells from primary foci. Following this track, we attempted to develop technology allowing to compare ultrastructure of these cells using correlative Transmission Electron Microscopy(TEM) and Low Voltage Scanning Electron Microscopy(LVSEM).


Author(s):  
Marek Malecki ◽  
James Pawley ◽  
Hans Ris

The ultrastructure of cells suspended in physiological fluids or cell culture media can only be studied if the living processes are stopped while the cells remain in suspension. Attachment of living cells to carrier surfaces to facilitate further processing for electron microscopy produces a rapid reorganization of cell structure eradicating most traces of the structures present when the cells were in suspension. The structure of cells in suspension can be immobilized by either chemical fixation or, much faster, by rapid freezing (cryo-immobilization). The fixation speed is particularly important in studies of cell surface reorganization over time. High pressure freezing provides conditions where specimens up to 500μm thick can be frozen in milliseconds without ice crystal damage. This volume is sufficient for cells to remain in suspension until frozen. However, special procedures are needed to assure that the unattached cells are not lost during subsequent processing for LVSEM or HVEM using freeze-substitution or freeze drying. We recently developed such a procedure.


Sign in / Sign up

Export Citation Format

Share Document