Processing of human melanoma cells for correlative TEM, LVSEM, and LM

1991 ◽  
Vol 49 ◽  
pp. 106-107
Author(s):  
Marek Malecki ◽  
J. Victor Small ◽  
James Pawley
Keyword(s):  
Electron Microscopy ◽  
Low Voltage ◽  
Fluorescent Microscopy ◽  
Blood Stream ◽  
Surface Topology ◽  
Integrin Receptors ◽  
Transmission Electron ◽  
Develop Technology ◽  
Voltage Scanning ◽  
Mechanisms Of Adhesion

The relative roles of adhesion and locomotion in malignancy have yet to be clearly established. In a tumor, subpopulations of cells may be recognized according to their capacity to invade neighbouring tissue,or to enter the blood stream and metastasize. The mechanisms of adhesion and locomotion are themselves tightly linked to the cytoskeletal apparatus and cell surface topology, including expression of integrin receptors. In our studies on melanomas with Fluorescent Microscopy (FM) and Cell Sorter(FACS), we noticed that cells in cultures derived from metastases had more numerous actin bundles, then cells from primary foci. Following this track, we attempted to develop technology allowing to compare ultrastructure of these cells using correlative Transmission Electron Microscopy(TEM) and Low Voltage Scanning Electron Microscopy(LVSEM).

A Novel Technique to Determine Platelet Deposition on an Experimental Nylon Membrane Using Lvsem and Tem

1998 ◽  
Vol 4 (S2) ◽  
pp. 848-849
Author(s):  
M.A. Murphy ◽  
J.P. DiOrio
Keyword(s):  
Electron Microscopy ◽  
Low Voltage ◽  
Filter Material ◽  
Nylon Membrane ◽  
Nylon Mesh ◽  
Transmission Electron ◽  
Nylon Fabric ◽  
Pass Through ◽  
Mesh Bag ◽  
Voltage Scanning

Porous nylon fabric is used as a filter in blood devices. This study was undertaken to evaluate the interaction between an experimental nylon filter and the cellular elements which pass through it. Our investigation was mainly centered on determining the deposition of cellular material on the nylon filter using Low Voltage Scanning Electron Microscopy (LVSEM) and Transmission Electron Microscopy (TEM).The filter was prepared for LVSEM by cutting a 2.5 cm2 of the nylon filter material with a razor and placing it in a nylon mesh bag which was stapled shut. The bag was rinsed three times for ten minutes each in fresh Sorensen's phosphate buffer. A graded alcohol series of 25%, 35%, 50%, 70%, 80%, and 90% was used to dehydrate the filter for ten minutes each. This was followed by dehydration twice for 20 minutes in fresh 100% ETOH. The filter was then Critical Point Dried (CPD) in the Tousimis AUTOSAMDRI-814.

Low-Voltage Scanning Electron Microscopy Investigation of Polymers

1988 ◽  
Vol 46 ◽  
pp. 220-221
Author(s):  
V. K. Berry
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Low Voltage ◽  
Morphological Characterization ◽  
Transmission Electron ◽  
Conducting Materials ◽  
Voltage Scanning ◽  
Scanning Electron

The morphological characterization of any polymer blend plays an important part in the development of a new blend system because the properties of blends are dictated by phase morphology which is dependent upon the chemistry and the processing conditions. Light microscopy, scanning electron microscopy and transmission electron microscopy are the most commonly used microscopical techniques for morphological characterization. Transmission electron microscopy techniques provide the best resolution (≈ 0.3 nm) but are limited in the size of sample area and require elaborate sample preparation procedures. Surface charging and beam damage problems have been some of the drawbacks of conventional scanning electron microscopy with non-conducting materials like polymers.The use of low accelerating voltage scanning electron microscopy (LVSEM) in the characterization of polymers and other non-conducting materials is beginning to be recognized.

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