NEMO-SNI real-time cabled seafloor observatory (southern Italy): operation assessment after two years from the deployment and next perspectives

Author(s):  
Paolo Favali ◽  
Laura Beranzoli ◽  
Franco Italiano ◽  
NEMO Collaboration
2017 ◽  
Vol 6 (1) ◽  
Author(s):  
Alice Vismarra ◽  
Elena Barilli ◽  
Maura Miceli ◽  
Carlo Mangia ◽  
Cristina Bacci ◽  
...  

Toxoplasmosis is a zoonotic disease caused by the protozoan <em>Toxoplasma gondii</em>. Ingestion of raw milk has been suggested as a risk for transmission to humans. Here the authors evaluated pre-treatment protocols for DNA extraction on <em>T. gondii</em> tachyzoite-spiked sheep milk with the aim of identifying the method that resulted in the most rapid and reliable PCR positivity. This protocol was then used to analyze milk samples form sheep from three different farms in southern Italy, including Real Time PCR for DNA quantification and PCR-RFLP for genotyping. The pre-treatment protocol using EDTA and Tris-HCl to remove casein gave the best results in the least amount of time compared to the others on spiked milk samples. One sample of 21 collected from sheep farms was positive on one-step PCR, Real Time PCR and resulted in a Type I genotype at one locus (SAG3). Milk usually contains a low number of tachyzoites and this could be a limiting factor for molecular identification. Our preliminary data has evaluated a rapid, cost-effective and sensitive protocol to treat milk before DNA extraction. The results of the present study also confirm the possibility of <em>T. gondii</em> transmission through consumption of raw milk and its unpasteurized derivatives.


2015 ◽  
pp. 211-228 ◽  
Author(s):  
K. Kawaguchi ◽  
S. Kaneko ◽  
T. Nishida ◽  
T. Komine

2019 ◽  
Vol 7 (11) ◽  
pp. 414 ◽  
Author(s):  
Yang Yu ◽  
Huiping Xu ◽  
Changwei Xu

Seafloor observatories enable continuous power supply and real-time bidirectional data transmission, which marks a new way for marine environment monitoring. As in situ observation produces massive data in a constant way, the research involved with data acquisition, data transmission, data analysis, and user-oriented data application is vital to the close-loop operations of seafloor observatories. In this paper, we design and implement a sensor web prototype (ESOSW) to resolve seafloor observatory information processing in a plug-and-play way. A sensor web architecture is first introduced, which is information-oriented and structured into four layers enabling bidirectional information flow of observation data and control commands. Based on the layered architecture, the GOE Control Method and the Hot Swapping Interpretation Method are proposed as the plug-and-play mechanism for sensor control and data processing of seafloor observatory networks. ESOSW was thus implemented with the remote-control system, the data management system, and the real-time monitoring system, supporting managed sensor control and on-demand measurement. ESOSW was tested for plug-and-play enablement through a series of trials and was put into service for the East China Sea Seafloor Observation System. The experiment shows that the sensor web prototype design and implementation are feasible and could be a general reference to related seafloor observatory networks.


Oceanography ◽  
2003 ◽  
Vol 16 (4) ◽  
pp. 53-59 ◽  
Author(s):  
Verena Tunnicliffe ◽  
Richard Dewey ◽  
Deborah Smith

Author(s):  
K. Kawaguchi ◽  
E. Araki ◽  
H. Matsumoto ◽  
Y. Kogure ◽  
T. Komine ◽  
...  

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