scholarly journals Thymidylate kinase of Mycobacterium tuberculosis: A chimera sharing properties common to eukaryotic and bacterial enzymes

2001 ◽  
Vol 10 (6) ◽  
pp. 1195-1205 ◽  
Author(s):  
HÉLÈNE Munier-Lehmann ◽  
Alain Chaffotte ◽  
Sylvie Pochet ◽  
Gilles Labesse
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Bacterial Enzymes ◽  
Thymidylate Kinase

Thymidine (monophosphate) analogues as Mycobacterium tuberculosis thymidylate kinase inhibitors

2002 ◽  
Author(s):  
Philippe Van Rompaey ◽  
Vanheusden Veerle ◽  
Sylvie Pochet ◽  
Hélene Munier-Lehmann ◽  
Matheus Froeyen ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Kinase Inhibitors ◽  
Thymidylate Kinase ◽  
Thymidine Monophosphate

scholarly journals Synthesis and evaluation of 5′-modified thymidines and 5-hydroxymethyl-2′-deoxyuridines as Mycobacterium tuberculosis thymidylate kinase inhibitors

2013 ◽  
Vol 21 (1) ◽  
pp. 257-268 ◽  
Author(s):  
Kiran S. Toti ◽  
Frederick Verbeke ◽  
Martijn D.P. Risseeuw ◽  
Vladimir Frecer ◽  
Hélène Munier-Lehmann ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Kinase Inhibitors ◽  
Thymidylate Kinase

Synthesis and Evaluation of Thymidine-5′-O-monophosphate Analogues as Inhibitors of Mycobacterium tuberculosis Thymidylate Kinase.

ChemInform ◽  
2003 ◽  
Vol 34 (2) ◽  
Author(s):  
Veerle Vanheusden ◽  
Helene Munier-Lehmann ◽  
Sylvie Pochet ◽  
Piet Herdewijn ◽  
Serge Van Calenbergh
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Thymidylate Kinase

Mycobacterium tuberculosis Thymidylate Kinase: Structural Studies of Intermediates along the Reaction Pathway

2003 ◽  
Vol 327 (5) ◽  
pp. 1077-1092 ◽  
Author(s):  
E. Fioravanti ◽  
A. Haouz ◽  
T. Ursby ◽  
H. Munier-Lehmann ◽  
M. Delarue ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Reaction Pathway ◽  
Structural Studies ◽  
Thymidylate Kinase

Thymidine and thymidine-5′-O-monophosphate analogues as inhibitors of Mycobacterium tuberculosis thymidylate kinase

2003 ◽  
Vol 13 (18) ◽  
pp. 3045-3048 ◽  
Author(s):  
Veerle Vanheusden ◽  
Philippe Van Rompaey ◽  
Hélène Munier-Lehmann ◽  
Sylvie Pochet ◽  
Piet Herdewijn ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Thymidylate Kinase

scholarly journals Mycobacterium Tuberculosis Thymidylate Kinase is A Potential 2 Diagnostic Biomarker for Pulmonary Tuberculosis

2020 ◽  
Author(s):  
Janet Peace Babirye ◽  
Charles Drago Kato ◽  
Fredrick Lutwama ◽  
Carol Musubika ◽  
Rose Nabatanzi ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Venous Blood ◽  
Area Under The Curve ◽  
Cross Sectional Study ◽  
Smear Microscopy ◽  
Diagnostic Biomarker ◽  
Cross Sectional ◽  
Thymidylate Kinase ◽  
Active Pulmonary Tuberculosis

Abstract Diagnosis of pulmonary tuberculosis (PTB) in context of HIV infection remains challenging due 25 to the paucibacillary nature of the disease. Mycobacterium tuberculosis thymidylate kinase 26 (TMKmt) has been validated as a novel biomarker with high detection limits for PTB in sputum. 27 We aimed to clinically test TMKmt as a diagnostic biomarker for pulmonary tuberculosis among 28 HIV positive individuals at Makerere University Joint AIDS Program Immuno Suppression 29 Syndrome clinic. 30 Methods 31 A total of 120 participants with presumptive PTB were enrolled in a cross- sectional study 32 between January and September 2018. Venous blood and expectorated spot sputum was obtained 33 from 116 consenting participants. Tuberculosis culture was performed on sputum as the gold 34 standard to confirm PTB status while direct ELISAs were performed on sputum and serum to 35 determine the level of TMKmt antigen. Sensitivity, specificity and receiver operator 36 characteristic curves were used to assess the precision of TMKmt in diagnosing PTB. 37 Results 38 Of the 116 participants, only 22 (19%) were PTB positive based on either Lowenstein Jensen or 39 Mycobacterial growth inhibition tube `culture. The mean sputum TMKmt levels were 40 significantly higher in PTB positive individuals (4.889 ± 0.135) ng/ml as compared to PTB 41 negative individuals (4.303 ± 0.07295) ng/ml (t-test, P<0.0005). At a cut off value of > 4.683 42 ng/ml, the test had a sensitivity of 73% (95% CI, 49.78% - 89.27%) and a specificity of 72% 43 (95% CI, 62.15% - 81.07%). These results were supported by a receiver operator analysis which 44 showed an area under the curve of 0.75 (95% CI, 0.63 – O.86). 3 45 Conclusion 46 Sputum TMKmt is a potential diagnostic biomarker for active pulmonary tuberculosis. This 47 forms an alternate test that would replace smear microscopy and overcome the current TB 48 diagnostic challenges.

Synthesis and evaluation of thymidine-5′-O-monophosphate analogues as inhibitors of Mycobacterium tuberculosis thymidylate kinase

2002 ◽  
Vol 12 (19) ◽  
pp. 2695-2698 ◽  
Author(s):  
Veerle Vanheusden ◽  
Hélène Munier-Lehmann ◽  
Sylvie Pochet ◽  
Piet Herdewijn ◽  
Serge Van Calenbergh
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Thymidylate Kinase

scholarly journals In silico Design and Synthesis of Tetrahydropyrimidinones and Tetrahydropyrimidinethiones as Potential Thymidylate Kinase Inhibitors Exerting Anti-TB Activity Against Mycobacterium tuberculosis

2020 ◽  
Vol Volume 14 ◽  
pp. 1027-1039 ◽  
Author(s):  
Katharigatta N Venugopala ◽  
Christophe Tratrat ◽  
Melendhran Pillay ◽  
Sandeep Chandrashekharappa ◽  
Omar Husham Ahmed Al-Attraqchi ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
In Silico ◽  
Kinase Inhibitors ◽  
Thymidylate Kinase ◽  
In Silico Design ◽  
Design And Synthesis

scholarly journals Mycobacterium tuberculosis thymidylate kinase antigen assays for designating incipient, high-risk latent M.tb infection

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Misaki Wayengera ◽  
David P. Kateete ◽  
Benon Asiimwe ◽  
Moses L. Joloba
Keyword(s):  
Mycobacterium Tuberculosis ◽  
High Risk ◽  
Thymidylate Kinase
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