A new insight into lead (II) tolerance of environmental fungi based on a study of Aspergillus niger and Penicillium oxalicum

2019 ◽  
Vol 21 (1) ◽  
pp. 471-479 ◽  
Author(s):  
Da Tian ◽  
Zhongquan Jiang ◽  
Liu Jiang ◽  
Mu Su ◽  
Zheye Feng ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Penicillium Oxalicum ◽  
Insight Into

scholarly journals The Lipomyces starkeyi gene Ls120451 encodes a cellobiose transporter that enables cellobiose fermentation in Saccharomyces cerevisiae

2020 ◽  
Vol 20 (3) ◽  
Author(s):  
Jorg C de Ruijter ◽  
Kiyohiko Igarashi ◽  
Merja Penttilä
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Aspergillus Niger ◽  
Heterologous Expression ◽  
In Silico ◽  
Penicillium Oxalicum ◽  
Microbial Fermentation ◽  
Lipomyces Starkeyi ◽  
Yeast Saccharomyces Cerevisiae ◽  
Sugar Transporters ◽  
Selection Of

ABSTRACT Processed lignocellulosic biomass is a source of mixed sugars that can be used for microbial fermentation into fuels or higher value products, like chemicals. Previously, the yeast Saccharomyces cerevisiae was engineered to utilize its cellodextrins through the heterologous expression of sugar transporters together with an intracellular expressed β-glucosidase. In this study, we screened a selection of eight (putative) cellodextrin transporters from different yeast and fungal hosts in order to extend the catalogue of available cellobiose transporters for cellobiose fermentation in S. cerevisiae. We confirmed that several in silico predicted cellodextrin transporters from Aspergillus niger were capable of transporting cellobiose with low affinity. In addition, we found a novel cellobiose transporter from the yeast Lipomyces starkeyi, encoded by the gene Ls120451. This transporter allowed efficient growth on cellobiose, while it also grew on glucose and lactose, but not cellotriose nor cellotetraose. We characterized the transporter more in-depth together with the transporter CdtG from Penicillium oxalicum. CdtG showed to be slightly more efficient in cellobiose consumption than Ls120451 at concentrations below 1.0 g/L. Ls120451 was more efficient in cellobiose consumption at higher concentrations and strains expressing this transporter grew slightly slower, but produced up to 30% more ethanol than CdtG.

Rutinosidase from Aspergillus niger : crystal structure and insight into the enzymatic activity

FEBS Journal ◽  
2020 ◽  
Vol 287 (15) ◽  
pp. 3315-3327 ◽  
Author(s):  
Petr Pachl ◽  
Jana Kapešová ◽  
Jiří Brynda ◽  
Lada Biedermannová ◽  
Helena Pelantová ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Aspergillus Niger ◽  
Enzymatic Activity ◽  
Insight Into

scholarly journals DETECCIÓN DE HONGOS TOXIGÉNICOS EN LA CADENA PRODUCTIVA DEL MAÍZ (Zea maíz L.)

2022 ◽  
Vol 7 (1) ◽  
pp. 50-53
Author(s):  
Vilma Julia Reyes De la Cruz ◽  
Emilio Fredy Yábar Villanueva
Keyword(s):  
Aspergillus Niger ◽  
Penicillium Oxalicum

Se ha determinado la presencia de hongos toxigénicos en los principales productos de la cadena productiva del maíz que son tierra de cultivo, choclo, maíz, maíz pelado y harina de maíz. Los cuales proceden de tres provincias del Departamento de Junín (Huancayo, Concepción y Chupaca). El objetivo de este trabajo fue cuantificar, aislar e identificar los hongos toxigénicos presentes en esta cadena, para proponer medidas de control y evitar su proliferación. Los hongos identificados fueron Penicillium oxalicum, P. viridicatum y P. digitatum en casi todo el estudio, Fusarium roseum y F. moniliforme especialmente en la tierra de cultivo; Aspergillus niger , Rizophus stolonifer y levaduras, especialmente en la harina de maíz. De ellos, Los hongos toxigénicos identificados fueron: F. moniliforme F. roseum, P. viridicatum y A. Níger, y los hongos que causan podredumbre fueron P. digitatum y P. oxalicum. La incidencia de hongos varió de 10 a 4,5 x 105 ufc/g siendo el promedio 4,2 x 104 ufc/g en toda la cadena. También se ha determinado que el maíz pelado y la harina de maíz presentan alto número de hongos especialmente aquellos procedentes de Huancayo, lo que indica que debe hacer un mejor manejo postcosecha y comercialización, ya que estos productos se expenden a granel con mayor riesgo de contaminación.

An insight into the curdione biotransformation pathway by Aspergillus niger

2014 ◽  
Vol 28 (7) ◽  
pp. 454-460 ◽  
Author(s):  
Yinan Chen ◽  
Lang Zhang ◽  
Bin Qin ◽  
Xin Zhang ◽  
Xian Jia ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Insight Into

scholarly journals Identification and Phylogenetic Analysis of Entomopathogenic Fungal Isolates Using Molecular Approach

Elkawnie ◽  
2020 ◽  
Vol 6 (2) ◽  
pp. 359
Author(s):  
Syaukani Syaukani ◽  
Zulia Ananda ◽  
Suhartono Suhartono ◽  
Sirtina Sirtina ◽  
Oviana Lisa ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Aspergillus Fumigatus ◽  
Entomopathogenic Fungi ◽  
Phylogenetic Trees ◽  
Pcr Amplification ◽  
Penicillium Oxalicum ◽  
Its Rdna ◽  
Molecular Approach ◽  
Relationship Of ◽  
The Relationship

Abstract: Entomopathogenic fungi are essential to consider as one of the biological agents to control termite populations. This research aimed to molecularly identify entomopathogenic fungi isolates in termites based on the ITS rDNA region and to determine the relationship of fungi isolates. Identification was performed by DNA extraction, PCR amplification, electrophoresis, purification, and sequencing. Phylogenetic trees were generated using MEGA X.  Molecular identification showed that the ISO1 sample was Penicillium oxalicum, the ISO2 sample was Trichoderma ghanense the ISO3 sample was Aspergillus niger, the ISO4 sample was Aspergillus fumigatus and the ISO5 sample was Aspergillus pseudonomius. The phylogenetic tree showed that the ISO1, ISO2, ISO3, ISO4, and ISO5 samples had the closest relationship with Penicillium oxalicum strain FR6-CGR12, Trichoderma ghanense isolate TM2, Aspergillus niger isolate 77, Aspergillus fumigatus, and Aspergillus pseudonomius strain DTO 267D6, respectively.Abstrak: Kelimpahan jenis fungi entomopatogen adalah hal yang terpenting untuk dipertimbangkan sebagai agen hayati bagi populasi rayap.Penelitian ini bertujuan untuk mengidentifikasi secara molekular isolat fungi entomopatogen pada rayap, berdasarkan daerah ITS rDNA dan mengetahui hubungan kekerabatan dari isolat fungi tersebut. Identifikasi dilakukan dengan cara ekstraksi DNA, amplifikasi menggunakan PCR, elektroforesis, purifikasi dan sekuensing. Selanjutnya kontruksi pohon filogenetik menggunakan aplikasi MEGA X. Berdasarkan uji molekular menunjukkan bahwa sampel ISO1 merupakan Penicillium oxalicum.ISO2 merupakan Trichoderma ghanense.ISO3 merupakan Aspergillus niger. ISO4 merupakan Aspergillus fumigatus.ISO5 merupakan Aspergillus pseudonomius.Konstruksi pohon filogenetik menunjukkan bahwa, sampel ISO1 berkerabat dekat dengan Penicillium oxalicum strain FR6-CGR12. Sampel ISO2 berkerabat dekat dengan Trichoderma ghanense isolat TM2. Sampel ISO3 berkerabat dekat dengan Aspergillus nigerisolat 77. Sampel ISO4 berkerabat dekat dengan Aspergillus fumigatus.Sampel ISO5 berkerabat dekat dengan Aspergillus pseudonomius strain DTO 267D6.

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