scholarly journals Pelagibacter metabolism of diatom‐derived volatile organic compounds imposes an energetic tax on photosynthetic carbon fixation

2019 ◽  
Vol 22 (5) ◽  
pp. 1720-1733 ◽  
Author(s):  
Eric R. Moore ◽  
Cleo L. Davie‐Martin ◽  
Stephen J. Giovannoni ◽  
Kimberly H. Halsey

2020 ◽  
Author(s):  
Volker Thiel ◽  
Jan-Peter Duda ◽  
Alfons M. van den Kerkhof ◽  
Joachim Reitner ◽  
Helge Mißbach

<p>The c. 3.5 Ga Dresser Formation of the East Pilbara Craton (Western Australia) contains large amounts of blackish barite. These rocks produce an intense sulfidic odor when crushed, resulting from abundant primary fluid inclusions. In part, the black barites are interbedded with sulfidic stromatolites. Using Raman spectroscopy, microthermometry, and two different online GC–MS approaches, we characterized in detail the chemical composition of the barite-hosted fluid inclusions. Our GC–MS techniques were based on (i) thermodecrepitation at 150-250°C and (ii) solid phase microextraction (SPME)–GC–MS at reduced temperature (50°C), thereby minimizing external contamination and artefact formation. Major fluid inclusion classes yielded mainly H<sub>2</sub>O, CO<sub>2</sub>, and H<sub>2</sub>S in varying abundance, along with minor amounts of COS and  CS<sub>2</sub>, N<sub>2</sub>, and CH<sub>4</sub> (< 1%). Notably, we also detected a wide range of volatile organic compounds, including short–chain ketones and aldehydes, thiophenes, and various organic (poly)sulfides. Some of these compounds (CH<sub>3</sub>SH, acetic acid) have previously been invoked as initials agents for carbon fixation under primordial conditions, but up to now their presence had not been observed in Precambrian materials. Based on our findings, we hypothesize that hydrothermal seepage of organic and inorganic compounds during Dresser times provided both, catabolic and anabolic substrates for early microbial metabolisms.</p>



Author(s):  
Gunnel Karlsson ◽  
Jan-Olov Bovin ◽  
Michael Bosma

RuBisCO (D-ribulose-l,5-biphosphate carboxylase/oxygenase) is the most aboundant enzyme in the plant cell and it catalyses the key carboxylation reaction of photosynthetic carbon fixation, but also the competing oxygenase reaction of photorespiation. In vitro crystallized RuBisCO has been studied earlier but this investigation concerns in vivo existance of RuBisCO crystals in anthers and leaves ofsugarbeets. For the identification of in vivo protein crystals it is important to be able to determinethe unit cell of cytochemically identified crystals in the same image. In order to obtain the best combination of optimal contrast and resolution we have studied different staining and electron accelerating voltages. It is known that embedding and sectioning can cause deformation and obscure the unit cell parameters.



2003 ◽  
Author(s):  
R. Boylstein ◽  
C. Piacitelli ◽  
G. Kullman ◽  
A. Grote




2017 ◽  
Vol 16 (3) ◽  
pp. 259-264 ◽  
Author(s):  
Rina Kim ◽  
◽  
Sungchul Seo ◽  
Joung-Dae Kim ◽  
Daekeun Kim




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