scholarly journals Unravelling lactate‐acetate and sugar conversion into butyrate by intestinal Anaerobutyricum and Anaerostipes species by comparative proteogenomics

2020 ◽  
Vol 22 (11) ◽  
pp. 4863-4875
Author(s):  
Sudarshan A. Shetty ◽  
Sjef Boeren ◽  
Thi P. N. Bui ◽  
Hauke Smidt ◽  
Willem M. Vos
Keyword(s):  
Fermentation ◽  
2018 ◽  
Vol 4 (3) ◽  
pp. 59 ◽  
Author(s):  
Tingting Liu ◽  
Shuangcheng Huang ◽  
Anli Geng

Cost-effective production of cellulosic ethanol requires robust microorganisms for rapid co-fermentation of glucose and xylose. This study aims to develop a recombinant diploid xylose-fermenting Saccharomyces cerevisiae strain for efficient conversion of lignocellulosic biomass sugars to ethanol. Episomal plasmids harboring codon-optimized Piromyces sp. E2 xylose isomerase (PirXylA) and Orpinomyces sp. ukk1 xylose (OrpXylA) genes were constructed and transformed into S. cerevisiae. The strain harboring plasmids with tandem PirXylA was favorable for xylose utilization when xylose was used as the sole carbon source, while the strain harboring plasmids with tandem OrpXylA was beneficial for glucose and xylose cofermentation. PirXylA and OrpXylA genes were also individually integrated into the genome of yeast strains in multiple copies. Such integration was beneficial for xylose alcoholic fermentation. The respiration-deficient strain carrying episomal or integrated OrpXylA genes exhibited the best performance for glucose and xylose co-fermentation. This was partly attributed to the high expression levels and activities of xylose isomerase. Mating a respiration-efficient strain carrying the integrated PirXylA gene with a respiration-deficient strain harboring integrated OrpXylA generated a diploid recombinant xylose-fermenting yeast strain STXQ with enhanced cell growth and xylose fermentation. Co-fermentation of 162 g L−1 glucose and 95 g L−1 xylose generated 120.6 g L−1 ethanol in 23 h, with sugar conversion higher than 99%, ethanol yield of 0.47 g g−1, and ethanol productivity of 5.26 g L−1·h−1.


2019 ◽  
Vol 7 ◽  
Author(s):  
Joana Pinto ◽  
Igor Pedrosa ◽  
Camila Linhares ◽  
Rosane A. S. San Gil ◽  
Yiu Lau Lam ◽  
...  

2019 ◽  
Vol 401 ◽  
pp. 213064 ◽  
Author(s):  
Noor Aljammal ◽  
Christia Jabbour ◽  
Joris W. Thybaut ◽  
Kristof Demeestere ◽  
Francis Verpoort ◽  
...  

2020 ◽  
Vol 22 (9) ◽  
pp. 2786-2798 ◽  
Author(s):  
Anna Sangregorio ◽  
Anitha Muralidhara ◽  
Nathanael Guigo ◽  
Lisbeth G. Thygesen ◽  
Guy Marlair ◽  
...  

Focus on humins, a co-product from sugar conversion, to highlight their capacity to impregnate wood cell walls for making durable wood products.


2012 ◽  
Vol 134 (24) ◽  
pp. 10089-10101 ◽  
Author(s):  
Filip de Clippel ◽  
Michiel Dusselier ◽  
Ruben Van Rompaey ◽  
Pieter Vanelderen ◽  
Jan Dijkmans ◽  
...  
Keyword(s):  

ACS Catalysis ◽  
2015 ◽  
Vol 5 (10) ◽  
pp. 5803-5811 ◽  
Author(s):  
Rik De Clercq ◽  
Michiel Dusselier ◽  
Charles Christiaens ◽  
Jan Dijkmans ◽  
Remus Ion Iacobescu ◽  
...  

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 620c-620
Author(s):  
L A. Merwin ◽  
G.D. Blanpied

Alternative groundcover management systems (GMS) beneath establishing 'Jonagold' apple trees have been evaluated since 1986 in an orchard field test Tree-row GMS being studied include post-emergence herbicide (glyphosate) “killed sods”, pre-emergence herbicides (norflurazon and diuron). a crownvetch “living mulch”, hay-straw mulch, monthly cultivation, and a closely mowed sodgrass. Trees in herbicide and straw-mulch plots produced the most fruit in 1990, but in 1991 there were no GMS-related differences in crop load. Fruit were larger in herbicide and straw-mulch plots in 1990 and 1991, and red color development was better in sod and crownvetch GMS. Starch-to-sugar conversion was advanced in apples from sodgrass and straw mulched plots. The ethylene climacteric was delayed in fruit from crownvetch and straw-mulch GMS, and peaked rapidly as mean starch indices approached 4.0 in all GMS treatments. Differences in soil moisture and nutrient availability under alternative GMS may have influenced fruit quality and maturity.


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