From the saltpan to the plate: an evaluation of the use of the edible halophyte Salicornia ramosissima in catering

Root−associated microbial communities play important roles in the process of adaptation of plant hosts to environment stressors, and in this perspective, the microbiome of halophytes represents a valuable model for understanding the contribution of microorganisms to plant tolerance to salt. Although considered as the most promising halophyte candidate to crop cultivation, Salicornia ramosissima is one of the least-studied species in terms of microbiome composition and the effect of sediment properties on the diversity of plant-growth promoting bacteria associated with the roots. In this work, we aimed at isolating and characterizing halotolerant bacteria associated with the rhizosphere and root tissues of S. ramosissima, envisaging their application in saline agriculture. Endophytic and rhizosphere bacteria were isolated from wild and crop cultivated plants, growing in different estuarine conditions. Isolates were identified based on 16S rRNA sequences and screened for plant-growth promotion traits. The subsets of isolates from different sampling sites were very different in terms of composition but consistent in terms of the plant-growth promoting traits represented. Bacillus was the most represented genus and expressed the wider range of extracellular enzymatic activities. Halotolerant strains of Salinicola, Pseudomonas, Oceanobacillus, Halomonas, Providencia, Bacillus, Psychrobacter and Brevibacterium also exhibited several plant-growth promotion traits (e.g., 3-indole acetic acid (IAA), 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, siderophores, phosphate solubilization). Considering the taxonomic diversity and the plant-growth promotion potential of the isolates, the collection represents a valuable resource that can be used to optimize the crop cultivation of Salicornia under different environmental conditions and for the attenuation of salt stress in non-halophytes, considering the global threat of arable soil salinization.

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Valorisation of Salicornia ramosissima biowaste by a green approach – An optimizing study using response surface methodology

Sustainable Chemistry and Pharmacy ◽

10.1016/j.scp.2021.100548 ◽

2021 ◽

Vol 24 ◽

pp. 100548

Author(s):

Diana Pinto ◽

Jorge Reis ◽

Ana Margarida Silva ◽

Miguel Salazar ◽

Stefano Dall’Acqua ◽

...

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Green Approach ◽

Salicornia Ramosissima

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Ecological differentiation of the microspecies Salicornia dolichostachya Moss and Salicornia ramosissima J. Woods

Vegetation between land and sea ◽

10.1007/978-94-009-4065-9_13 ◽

1987 ◽

pp. 164-178 ◽

Cited By ~ 5

Author(s):

H. Schat ◽

J. C. Van Der List ◽

J. Rozema

Keyword(s):

Ecological Differentiation ◽

Salicornia Ramosissima

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Investigating the physiological mechanisms underlying Salicornia ramosissima response to atmospheric CO2 enrichment under coexistence of prolonged soil flooding and saline excess

Plant Physiology and Biochemistry ◽

10.1016/j.plaphy.2018.12.003 ◽

2019 ◽

Vol 135 ◽

pp. 149-159 ◽

Cited By ~ 7

Author(s):

Jesús Alberto Pérez-Romero ◽

Bernardo Duarte ◽

Jose-Maria Barcia-Piedras ◽

Ana Rita Matos ◽

Susana Redondo-Gómez ◽

...

Keyword(s):

Co2 Enrichment ◽

Atmospheric Co2 ◽

Physiological Mechanisms ◽

Soil Flooding ◽

Salicornia Ramosissima

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Aquaponics using a fish farm effluent shifts bacterial communities profile in halophytes rhizosphere and endosphere

Scientific Reports ◽

10.1038/s41598-020-66093-8 ◽

2020 ◽

Vol 10 (1) ◽

Cited By ~ 1

Author(s):

Vanessa Oliveira ◽

Patrícia Martins ◽

Bruna Marques ◽

Daniel F. R. Cleary ◽

Ana I. Lillebø ◽

...

Keyword(s):

Natural Environment ◽

Bacterial Communities ◽

Denaturing Gradient Gel Electrophoresis ◽

Nutrient Loads ◽

Marine Aquaculture ◽

Aquaculture Effluents ◽

Specific Effects ◽

Salicornia Ramosissima ◽

Gradient Gel Electrophoresis ◽

Species Specific

AbstractThe intensification of marine aquaculture raises multiple sustainability issues, namely the handling of nutrient-rich effluents that can adversely impact ecosystems. As integrated multi-trophic aquaculture (IMTA) gains momentum, the use of halophyte plants to phytoremediate aquaculture effluents has received growing attention, particularly in aquaponics. It is, therefore, important to obtain a more in-depth knowledge of the microbial communities present in the root systems of these plants, both in their natural environment (sediment) and in aquaponics, in order to understand their nutrient removal potential. The present study used denaturing gradient gel electrophoresis (DGGE) and barcoded pyrosequencing to assess the bacterial community present in the endosphere and rhizosphere of three halophyte plants: Halimione portulacoides, Salicornia ramosissima and Sarcocornia perennis. Species-specific effects were recorded in the profile and diversity of the bacterial communities present in halophyte roots, with significant differences also recorded for the same halophyte species grown in contrasting environments (sediment vs. aquaponics). In aquaponics the most abundant groups belonged to the orders Rhodocyclales, Campylobacterales, Rhodobacterales and Desulfobacterales, while in the natural environment (sediment) the most abundant groups belonged to the orders Rhizobiales, Sphingomonadales and Alteromonadales. An overall enrichment in bacterial taxa involved in nutrient cycling was recorded in the roots of halophytes grown in aquaponics (such as Denitromonas, Mesorhizobium, Colwellia, Dokdonella and Arcobacter), thereby highlighting their potential to reduce the nutrient loads from aquaculture effluents.

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