Paternal factors and embryonic development: Role in recurrent pregnancy loss

Andrologia ◽  
2018 ◽  
Vol 51 (1) ◽  
pp. e13171 ◽  
Author(s):  
Vidhu Dhawan ◽  
Manoj Kumar ◽  
Dipika Deka ◽  
Neena Malhotra ◽  
Neeta Singh ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Paternal Factors

Molecular mechanisms of imprinted gene disturbance in the embryonic development pathology and recurrent pregnancy loss

2020 ◽  
pp. 79-80
Author(s):  
Е.А. Саженова ◽  
И.Н. Лебедев
Keyword(s):  
Embryonic Development ◽  
Pregnancy Loss ◽  
Molecular Mechanisms ◽  
Recurrent Pregnancy Loss ◽  
Gene Mutations ◽  
Spontaneous Abortions ◽  
Imprinted Gene ◽  
Heterozygous Carriers

Установлено, что остановка эмбрионального развития человека сопровождается мультилокусными дефектами метилирования импринтированных генов, частота которых повышена в плацентарных тканях спонтанных абортусов от женщин с привычным невынашиванием беременности. Показано, что множественные дефекты метилирования импринтированных генов сопровождаются мутациями гена NLRP7 у спонтанных абортусов от женщин с привычным невынашиванием беременности, а родители являются гетерозиготными носителями данных мутаций. It was found that disturbances of embryonic development are accompanied by multi-locus imprinting disturbance (MLID), the frequency of which is increased in placental tissues of spontaneous abortions from women with recurrent pregnancy loss. It is shown that MLID are accompanied by NLRP7 gene mutations in spontaneous abortions from women with recurrent pregnancy loss, and parents are heterozygous carriers of these mutations.

Proteomic and functional analysis of proteins related to embryonic development of decidua in patients with recurrent pregnancy loss

2021 ◽  
Author(s):  
Yi-Meng Xiong ◽  
Hai-Tao Pan ◽  
Hai-Gang Ding ◽  
Yao He ◽  
Juan Zhang ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Embryonic Development ◽  
Pathway Analysis ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Comparative Proteomics ◽  
Differentially Expressed Proteins ◽  
Biological Mechanisms ◽  
Protein Protein Interaction ◽  
Upstream Regulator

Abstract Recurrent pregnancy loss (RPL) is defined as the loss of two or more consecutive pregnancies before the 20 weeks of gestation. Recurrent pregnancy loss affects about 1–2% of couples trying to conceive; however, the mechanisms leading to this complication are largely unknown. Our previous studies using comparative proteomics identified 314 differentially expressed proteins (DEPs) in the placental villous. In this study, we identified 5479 proteins from a total of 34,157 peptides in decidua of patients with early recurrent pregnancy loss (Data are available via ProteomeXchange with identifier PXD023849). Further analysis identified 311 DEPs in the decidua tissue; and 159 proteins were highly expressed while 152 proteins were lowly expressed. These 311 proteins were further analyzed by using Ingenuity Pathway Analysis (IPA). The results suggested that 50 DEPs played important roles in the embryonic development. Upstream analysis of these DEPs revealed that AGT was the most important upstream regulator. Furthermore, protein - protein interaction (PPI) analysis of the embryonic development DEPs from the placental villous and decidua was performed in the STRING database. This study identified several proteins specifically associated with embryonic development in decidua of patients with early recurrent pregnancy loss. Therefore, these results provide new insights into potential biological mechanisms, that may ultimately inform recurrent pregnancy loss.

scholarly journals Proteomic Signatures in Spermatozoa Reveal the Role of Paternal Factors in Recurrent Pregnancy Loss

2020 ◽  
Vol 38 (1) ◽  
pp. 103 ◽  
Author(s):  
Gayatri Mohanty ◽  
Soumya Ranjan Jena ◽  
Jasmine Nayak ◽  
Sujata Kar ◽  
Luna Samanta
Keyword(s):  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Paternal Factors

scholarly journals Evaluating the role of paternal factors in aetiology and prognosis of recurrent pregnancy loss: study protocol for a hospital-based multicentre case–control study and cohort study (REMI III project)

BMJ Open ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. e033095 ◽  
Author(s):  
Nadia du Fossé ◽  
Marie-Louise van der Hoorn ◽  
Michael Eikmans ◽  
Sebastiaan Heidt ◽  
Saskia le Cessie ◽  
...  
Keyword(s):  
Cohort Study ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Medical Center ◽  
Case Control ◽  
Paternal Age ◽  
Time Interval ◽  
Maternal Risk ◽  
Paternal Factors

IntroductionRecurrent pregnancy loss (RPL) is defined as the spontaneous demise of two or more pregnancies before the fetus reaches viability. Despite investigation of multiple known maternal risk factors, in more than 50% of couples, this condition remains unexplained. Studies focusing on paternal factors in RPL are scarce, and therefore, paternal evaluation in RPL is currently very limited. However, regarding single miscarriage, there are multiple publications suggesting a contributive role of paternal factors. In this project, we aim to identify paternal factors associated with RPL and to improve couple-specific prediction of future pregnancy outcomes by developing a prediction model containing both maternal and paternal factors.Methods and analysisIn a case–control design, the relation between unexplained RPL and paternal age, lifestyle factors, sperm DNA damage and immunomodulatory factors in peripheral blood and semen will be studied. Prospectively, 135 couples with naturally conceived unexplained RPL (cases) and 135 fertile couples without a history of pregnancy loss (controls) will be included, with collection of paternal blood and semen samples and documentation of clinical and lifestyle characteristics. In addition, 600 couples from both groups will be included retrospectively. To adjust for confounders, multivariate logistic regression will be used. The predictive value of paternal and maternal factors will be studied in the total RPL cohort consisting of approximately 735 couples. The primary outcome of the cohort study is live birth within 5 years after initial visit of the clinic. Secondary outcomes are ongoing pregnancy, time interval until next pregnancy and pregnancy complications.Ethics and disseminationThis project is approved by the Medical Research Ethics Committee of the Leiden University Medical Center. No risks or burden are expected from the study. The findings of this study will be disseminated via peer-reviewed publications and presentations at international conferences.Trial registration numberNL7762

scholarly journals Prediction key genes and the potential molecular mechanism for early recurrent pregnancy loss by Multi-Omics analysis

2020 ◽  
Author(s):  
Le Huang ◽  
Changqiang Wei ◽  
Zhao Zhang ◽  
Jiao Cen ◽  
Zhiwei Zhu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Embryonic Development ◽  
Early Pregnancy ◽  
Regulatory Networks ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Gene Expression Omnibus ◽  
Potential Mechanism ◽  
Omics Analysis ◽  
Key Genes

Abstract BackgroundThe potential mechanism of early recurrent pregnancy loss (ERPL) has not been fully elucidated, a multi-omics analysis can help us find key genes.ResultsWe download data from Gene Expression Omnibus (GEO), 90 hypermethylation-down regulated genes and 49 hypomethylation-up regulated genes were identified by intersection. Compared with the normal early pregnancy group, the expression of ERCC2 and MLH1 was lower in ERPL group, but the expression of PLEK and FOS was higher, and the expression of MLH1 was statistically significant (p<0.05). Compared with the anembryonic (empty sac) miscarriage group, the expression of MLH1, PLEK, FOS decreased, and that of ERCC2 increased in the embryonic miscarriage group. TF-MDEGs networks predicted SP1, POLR2A, YY1, CREM and CREB1 were involved in methylation regulation of DNA promoter with MDEGs. Among them, YY1, FOXP3 and p53 may be related to the mechanism of MLH1 in ERPL.ConclusionsOur study identified possible aberrant MDEGs, and TF- MDEGs regulatory networks may be related to its mechanism. MLH1 may play an important role in early embryonic development.

scholarly journals Prediction key genes and the potential molecular mechanism for early recurrent pregnancy loss by Multi-Omics analysis

2020 ◽  
Author(s):  
Le Huang ◽  
Changqiang Wei ◽  
Zhao Zhang ◽  
Jiao Cen ◽  
Zhiwei Zhu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Embryonic Development ◽  
Early Pregnancy ◽  
Regulatory Networks ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Gene Expression Omnibus ◽  
Potential Mechanism ◽  
Omics Analysis ◽  
Key Genes

Abstract Background: The potential mechanism of early recurrent pregnancy loss (ERPL) has not been fully elucidated, a multi-omics analysis can help us find key genes.Results: We download data from Gene Expression Omnibus (GEO), 90 hypermethylation-down regulated genes and 49 hypomethylation-up regulated genes were identified by intersection. Compared with the normal early pregnancy group, the expression of ERCC2 and MLH1 was lower in ERPL group, but the expression of PLEK and FOS was higher, and the expression of MLH1 was statistically significant (p<0.05). Compared with the anembryonic (empty sac) miscarriage group, the expression of MLH1, PLEK, FOS decreased, and that of ERCC2 increased in the embryonic miscarriage group. TF-MDEGs networks predicted SP1, POLR2A, YY1, CREM and CREB1 were involved in methylation regulation of DNA promoter with MDEGs. Among them, YY1, FOXP3 and p53 may be related to the mechanism of MLH1 in ERPL.Conclusions: Our study identified possible aberrant MDEGs, and TF- MDEGs regulatory networks may be related to its mechanism. MLH1 may play an important role in early embryonic development.

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