scholarly journals Normal fertility with deletion of sY 84 and sY 86 in AZF a region

Andrology ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 332-336
Author(s):  
D. Tang ◽  
W. Liu ◽  
G. Li ◽  
X. He ◽  
Z. Zhang ◽  
...  
Keyword(s):  
Normal Fertility

scholarly journals Expression of Human Hormone-Sensitive Lipase (HSL) in Postmeiotic Germ Cells Confers Normal Fertility to HSL-Deficient Mice

Endocrinology ◽  
2004 ◽  
Vol 145 (12) ◽  
pp. 5688-5693 ◽  
Author(s):  
Shu Pei Wang ◽  
Shari Chung ◽  
Krishnakant Soni ◽  
Hugo Bourdages ◽  
Louis Hermo ◽  
...  
Keyword(s):  
Germ Cells ◽  
Free Cholesterol ◽  
Fatty Acyl ◽  
Hormone Sensitive Lipase ◽  
Cortical Cells ◽  
Cholesterol Ratio ◽  
Testicular Mass ◽  
Normal Fertility ◽  
Hormone Sensitive ◽  
Testicular Histology

Abstract Hormone-sensitive lipase (HSL, Lipe, E.C.3.1.1.3) is a multifunctional fatty acyl esterase that is essential for male fertility and spermatogenesis and that also plays important roles in the function of adipocytes, pancreatic β-cells, and adrenal cortical cells. Gene-targeted HSL-deficient (HSL−/−) male mice are infertile, have a 2-fold reduction in testicular mass, a 2-fold elevation of the ratio of esterified to free cholesterol in testis, and unique morphological abnormalities in round and elongating spermatids. Postmeiotic germ cells in the testis express a specific HSL isoform. We created transgenic mice expressing a normal human testicular HSL cDNA from the mouse protamine-1 promoter, which mediates expression specifically in postmeiotic germ cells. Testicular cholesteryl esterase activity was undetectable in HSL−/− mice, but in HSL−/− males expressing the testicular transgene, activity was 2-fold greater than normal. HSL transgene mRNA became detectable in testes between 19 and 25 days of age, coinciding with the first wave of postmeiotic transcription in round spermatids. In contrast to nontransgenic HSL−/− mice, HSL−/− males expressing the testicular transgene were normal with respect to fertility, testicular mass, testicular esterified/free cholesterol ratio, and testicular histology. Their cauda epididymides contained abundant, normal-appearing spermatozoa. We conclude that human testicular HSL is functional in mouse testis and that the mechanism of infertility in HSL-deficient males is cell autonomous and resides in postmeiotic germ cells, because HSL expression in these cells is in itself sufficient to restore normal fertility.

Progesterone receptors in AVPV kisspeptin neurons are sufficient for positive feedback induction of the LH surge

Endocrinology ◽  
2021 ◽  
Author(s):  
Margaret A Mohr ◽  
Lourdes A Esparza ◽  
Paige Steffen ◽  
Paul E Micevych ◽  
Alexander S Kauffman
Keyword(s):  
Luteinizing Hormone ◽  
Transgenic Mice ◽  
Positive Feedback ◽  
Progesterone Receptors ◽  
Critical Importance ◽  
Estrogen And Progesterone Receptors ◽  
Lh Surge ◽  
Normal Fertility ◽  
Gnrh Neurons ◽  
The Brain

Abstract Kisspeptin, encoded by Kiss1, stimulates GnRH neurons to govern reproduction. In female rodents, estrogen-sensitive kisspeptin neurons in the rostral anteroventral periventricular (AVPV) hypothalamus are thought to mediate estradiol (E2)-induced positive feedback induction of the preovulatory luteinizing hormone (LH) surge. AVPV kisspeptin neurons co-express estrogen and progesterone receptors (PGR) and are activated during the LH surge. While E2 effects on kisspeptin neurons have been well-studied, progesterone’s regulation of kisspeptin neurons is less understood. Using transgenic mice lacking PGR exclusively in kisspeptin cells (termed KissPRKOs), we previously demonstrated that progesterone action specifically in kisspeptin cells is essential for ovulation and normal fertility. Unlike control females, KissPRKO females did not generate proper LH surges, indicating that PGR signaling in kisspeptin cells is required for proper positive feedback. However, since PGR was knocked out from all kisspeptin neurons in the brain, that study was unable to determine the specific kisspeptin population mediating PGR action on the LH surge. Here, we used targeted Cre-mediated AAV technology to re-introduce PGR selectively into AVPV kisspeptin neurons of adult KissPRKO females, and tested whether this rescues occurrence of the LH surge. We found that targeted upregulation of PGR in kisspeptin neurons exclusively in the AVPV is sufficient to restore proper E2-induced LH surges in KissPRKO females, suggesting that this specific kisspeptin population is a key target of the necessary progesterone action for the surge. These findings further highlight the critical importance of progesterone signaling, along with E2 signaling, in the positive feedback induction of LH surges and ovulation.

Double translocation heterozygosity and normal fertility in domestic sheep

1974 ◽  
Vol 13 (4) ◽  
pp. 342-351 ◽  
Author(s):  
A.N. Bruère ◽  
H.M. Chapman
Keyword(s):  
Domestic Sheep ◽  
Normal Fertility ◽  
Translocation Heterozygosity

scholarly journals Polygenic expression of teratozoospermia and normal fertility in B 10. MOL ‐ TEN 1 mouse strain

2015 ◽  
Vol 55 (2) ◽  
pp. 92-98 ◽  
Author(s):  
Keitaro Hirawatari ◽  
Naoto Hanzawa ◽  
Maki Kuwahara ◽  
Hiroaki Aoyama ◽  
Ikuo Miura ◽  
...  
Keyword(s):  
Mouse Strain ◽  
Normal Fertility

Comparison of heat-treated and tetraploid blastocysts for the production of completely ES-cell-derived mice

Zygote ◽  
2001 ◽  
Vol 9 (2) ◽  
pp. 153-157 ◽  
Author(s):  
T. Amano ◽  
Y. Kato ◽  
Y. Tsunoda
Keyword(s):  
Germ Cells ◽  
Genetic Background ◽  
Production Efficiency ◽  
Es Cells ◽  
Embryonic Stem ◽  
Treated Group ◽  
Clear Correlation ◽  
Es Cell ◽  
Normal Fertility ◽  
Heat Treated

The present study compared the production efficiency and incidence of postnatal death in mice derived by injecting embryonic stem (ES) cells into either heat-treated blastocysts or tetraploid blastocysts. The proportion of completely ES-cell-derived mice from the tetraploid blastocyst group (3.3%) was significantly higher than that obtained from the heat-treated blastocyst group (1.5%). The incidence of postnatal death was the same between the two groups: 10 of 15 young (67%) in the heat-treated group and 21 of 34 young (62%) in the tetraploid group died within 13 days of birth. The remaining young grew to adulthood, had normal fertility, and their germ cells were of ES cell origin. There was no clear correlation, however, between the postnatal lethality of ES-cell-derived mice and the genetic background of the ES cells. The causes of postnatal death are discussed.

Normal fertility in male mice with deletion of β-catenin gene in germ cells

genesis ◽  
2014 ◽  
Vol 52 (4) ◽  
pp. 328-332 ◽  
Author(s):  
Bryan Rivas ◽  
Zaohua Huang ◽  
Alexander I. Agoulnik
Keyword(s):  
Germ Cells ◽  
Male Mice ◽  
Normal Fertility

294 EFFECTS OF FOLLICULAR FLUID OBTAINED FROM REPEAT BREEDER DAIRY HEIFER ON MATURATION OF BOVINE OOCYTES IN VITRO

2015 ◽  
Vol 27 (1) ◽  
pp. 236
Author(s):  
M. Kafi ◽  
M. R. Divar ◽  
S. Gharib-Zadeh
Keyword(s):  
Follicular Fluid ◽  
Present Experiment ◽  
Calf Serum ◽  
Low Fertility ◽  
Control Group ◽  
Normal Fertility ◽  
Maturation Rate ◽  
Bovine Oocytes ◽  
Repeat Breeder

The cause of repeat breeding syndrome is often difficult to explain in dairy heifers with no clinical abnormalities. The aim of the present experiment was to determine the effect of follicular fluid obtained from the preovulatory follicle of repeat breeder heifers on maturation of bovine oocytes in vitro. Holstein virgin heifers either with normal fertility (VH, n = 5) or repeat breeder syndrome (RB, n = 5) were used in the present experiment. The RB heifers had a history of at least 5 unsuccessful consequent artificial breeding. The reason for using such RB heifers was to exclude the possibility of the presence of usual causes of infertility in heifers. Oestrus cycles of all heifers were synchronized using 2 injections of PGF2a 11 days apart. Six to 12 h after oestrus detection, clear follicular fluid samples from the ovulatory follicles were collected transrectally using a long fine-needle covered by a hard plastic tube. Follicular fluid samples were pooled, centrifuged, and frozen until used in the maturation medium. A total of 483 good or excellent quality bovine cumulus-oocytes complexes (COC) were obtained from 2 to 6 mm follicles in diameter from slaughterhouse ovaries and randomly allocated in 3 groups; in group 1 (control, n = 180), oocytes were cultured in TCM-199 supplemented with 10% heat-treated fetal calf serum and hormones (5 IU mL–1 of hCG plus 0.1 IU mL–1 of rFSH); in group 2 (n = 126), oocytes were cultured in TCM-199 supplemented with 10% filtered follicular fluid of VH without hormones; in group 3 (n = 177), oocytes were cultured in TCM-199 supplemented with 10% filtered follicular fluid of RB heifers without hormones. All oocytes were cultured for 24 h at 39°C in an atmosphere of 5% CO2 under 90% humidity. At the end of maturation, the degree of cumulus expansion was evaluated and scored under a stereomicroscope. Then, oocytes were mechanically denuded using 3% sodium citrate and repeated pipetting and were fixed in ethanol/acetic acid (3 : 1) for 24 h. The oocytes were subsequently stained with 1% aceto-orcein and evaluated for meiotic resumption. Proportions were statistically analysed using a Chi-squared test (significant at P < 0.05; SPSS program, 11.5). The percentages of fully expanded COC differed among groups (P < 0.001). The maturation rate (MII stage) was 83% (150/180) in oocytes that were cultured in the presence of FCS as the control group. However, a reduction in the maturation rate was observed when oocytes were cultured either in VH follicular fluid (71.4%, 90/126; P < 0.01) or RB follicular fluid (59.3%, 105/177; P < 0.001) compared to the control group. The percentages of matured oocytes were also different between VH and RB follicular fluid (71.4 v. 59.3%; P < 0.01, respectively). In conclusion, the quality of follicular fluid of the preovulatory follicles of repeat breeder heifers is lower than that of the virgin heifers with normal fertility. This may explain the cause of the low fertility in some repeat breeder Holstein heifers.

scholarly journals Autophagy core protein ATG5 is required for elongating spermatid development, sperm individualization and normal fertility in male mice

Autophagy ◽  
2020 ◽  
pp. 1-15 ◽  
Author(s):  
Qian Huang ◽  
Yunhao Liu ◽  
Shiyang Zhang ◽  
Yi Tian Yap ◽  
Wei Li ◽  
...  
Keyword(s):  
Core Protein ◽  
Male Mice ◽  
Normal Fertility
Sign in / Sign up

Export Citation Format

Share Document