Amino acid absorption and protein synthesis responses of turbot Scophthalmus maximus to lysine and leucine in free, dipeptide and tripeptide forms

2019 ◽  
Vol 26 (2) ◽  
pp. 358-367 ◽  
Author(s):  
Yuliang Wei ◽  
Houguo Xu ◽  
Mengqing Liang
Keyword(s):  
Protein Synthesis ◽  
Amino Acid ◽  
Scophthalmus Maximus ◽  
Acid Absorption ◽  
Amino Acid Absorption ◽  
Turbot Scophthalmus Maximus

scholarly journals Ingestion of Free Amino Acids as Opposed to Intact Protein Increases Amino Acid Absorption but Does Not Further Augment Postprandial Muscle Protein Synthesis Rates

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 673-673
Author(s):  
Michelle E G Weijzen ◽  
Rob JJ van Gassel ◽  
Imre W K Kouw ◽  
Stefan H M Gorissen ◽  
Marcel CG van de Poll ◽  
...  
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Amino Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Muscle Protein ◽  
Muscle Protein Synthesis ◽  
Protein Digestion ◽  
Acid Absorption ◽  
Amino Acid Absorption

Abstract Objectives The rate of protein digestion and amino acid absorption determines the postprandial rise in circulating amino acids and, as such, modulates postprandial muscle protein synthesis rates. This study compares protein digestion and amino acid absorption kinetics and the subsequent muscle protein synthetic response following ingestion of intact protein versus an equivalent amount of free, crystalline amino acids. Methods Twenty-four healthy, young subjects (age: 22 ± 3 y, BMI: 23 ± 2 kg·m−2, sex: 12 M/12F) ingested 30 g intrinsically L-[1–13C]-phenylalanine and L-[1–13C]-leucine labeled milk protein (PROT; n = 12) or an equivalent amount of free amino acids (AA; n = 12). In addition, subjects received primed continuous L-[ring-2H5]-phenylalanine, L-[ring-3,5–2H2]-tyrosine, and L-[1–13C]-leucine infusions. Blood samples and muscle biopsies were obtained frequently to assess protein digestion and amino acid absorption kinetics and subsequent muscle protein synthesis rates over a 6 h postprandial period. An unpaired t-test was used to compare overall exogenous phenylalanine release in plasma. For other parameters repeated measures ANOVA were applied to determine differences between groups over time (time as within, and group as between-subjects factor). Data are expressed as mean ± SD. Results Postprandial plasma amino acid concentrations and exogenous phenylalanine appearance rates increased after ingestion of PROT and AA (both, P < 0.001), with a greater increase following ingestion of AA when compared to PROT (time*group interaction P < 0.001). Exogenous phenylalanine release in plasma assessed over the 6 h postprandial period, was greater in AA (76 ± 9%) compared with PROT (59 ± 10%; P < 0.001). Ingestion of AA and PROT strongly increased muscle protein synthesis rates based upon L-[ring-2H5]-phenylalanine (time effect P < 0.001), with no differences between groups (from 0.037 ± 0.015 to 0.053 ± 0.014%·h−1 and from 0.039 ± 0.016 to 0.051 ± 0.010%·h−1, respectively; time*group interaction P = 0.629). Conclusions Ingestion of free amino acids as opposed to intact milk protein is followed by more rapid amino acid absorption and greater postprandial plasma amino acid availability, but this does not further augment postprandial muscle protein synthesis rates. Funding Sources This research did not receive external funding.

Energy for amino acid absorption, transport and protein synthesis in vivo

1978 ◽  
Vol 60 (1) ◽  
pp. 13-20 ◽  
Author(s):  
Roland A Coulson ◽  
Jack D Herbert ◽  
Hernandez Thomas
Keyword(s):  
Protein Synthesis ◽  
Amino Acid ◽  
Acid Absorption ◽  
Amino Acid Absorption

Actinomycin D Effect on Amino Acid Absorption from Rat Jejunal Loops

Science ◽  
1967 ◽  
Vol 158 (3797) ◽  
pp. 129-130 ◽  
Author(s):  
C. Yamada ◽  
A. J. Clark ◽  
M. E. Swendseid
Keyword(s):  
Amino Acid ◽  
Actinomycin D ◽  
Acid Absorption ◽  
Amino Acid Absorption

Influence of cytochalasins on sugar and amino acid absorption in the mouse

Toxicon ◽  
1983 ◽  
Vol 21 ◽  
pp. 173-176 ◽  
Author(s):  
T. Glinsukon ◽  
C. Toskulkao ◽  
W. Wangpanish ◽  
M. Chulasamaya
Keyword(s):  
Amino Acid ◽  
Acid Absorption ◽  
Amino Acid Absorption

scholarly journals Amino Acid Absorption in the Large Intestine of Humans and Porcine Models

2017 ◽  
Vol 147 (8) ◽  
pp. 1493-1498 ◽  
Author(s):  
Nikkie van der Wielen ◽  
Paul J Moughan ◽  
Marco Mensink
Keyword(s):  
Amino Acid ◽  
Large Intestine ◽  
Acid Absorption ◽  
Amino Acid Absorption
Sign in / Sign up

Export Citation Format

Share Document