Effect of dietary methionine levels on growth performance, amino acid metabolism and intestinal homeostasis in turbot (Scophthalmus maximus L.)

Aquaculture ◽  
2019 ◽  
Vol 498 ◽  
pp. 335-342 ◽  
Author(s):  
Zhongyue Gao ◽  
Xuan Wang ◽  
Chang Tan ◽  
Huihui Zhou ◽  
Kangsen Mai ◽  
...  
Keyword(s):  
Amino Acid ◽  
Growth Performance ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Scophthalmus Maximus ◽  
Intestinal Homeostasis ◽  
Turbot Scophthalmus Maximus

Amino acid metabolism and protein turnover in larval turbot ( Scophthalmus maximus ) fed natural zooplankton or Artemia

1997 ◽  
Vol 129 (2) ◽  
pp. 255-265 ◽  
Author(s):  
L. E. C. Conceição ◽  
T. van der Meeren ◽  
J. A. J. Verreth ◽  
M. S. Evjen ◽  
D. F. Houlihan ◽  
...  
Keyword(s):  
Amino Acid ◽  
Protein Turnover ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Scophthalmus Maximus ◽  
Turbot Scophthalmus Maximus

scholarly journals PSIII-23 Characterization of oxidized oil-elicited disruption of amino acid metabolism in nursery pigs through metabolomic analysis

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 177-177
Author(s):  
Yue Guo ◽  
Andrea Hanson ◽  
Lei Wang ◽  
Brian Kerr ◽  
Pedro Urriola ◽  
...  
Keyword(s):  
Amino Acid ◽  
Growth Performance ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Corn Oil ◽  
Phase 1 ◽  
Average Daily Gain ◽  
Metabolomics Data ◽  
Nursery Pigs ◽  
Metabolomics Analysis

Abstract Feeding oxidized lipids compromises growth performance of pigs, but the metabolic events contributing to this adverse effect are not well defined. In this study, oxidized corn oil (OCO) was prepared by heating control corn oil (CCO) at 185 oC for 12 h. Weanling pigs (initial BW = 6.3 ± 1.4 kg) were fed 4 isocaloric diets containing 9% CCO, 6% CCO + 3% OCO, 3% CCO + 6% OCO, and 9% OCO, respectively. Pigs were provided ad libitum access to experimental diets in 3 phases (phase 1 = 4 d, phase 2 = 10 d and phase 3 = 21 d) for 35 d. Pig body weight (BW) and feed disappearance were determined at the d 0, 4, 14 and 35 to calculate average daily gain (ADG), average daily feed intake (ADFI) and gain to feed (G:F) ratio. Serum and liver samples collected on d 35 of feeding were analyzed by the liquid chromatography-mass spectrometry (LC-MS)-based metabolomics analysis. Growth performance data were analyzed using the MIXED procedure of SAS and metabolomics data were analyzed by two-tailed student’s t test for comparison between different doses of OCO and CCO treatments. The result showed that dietary OCO decreased G:F ratio (P < 0.05) dose-dependently, but did not ADFI. Metabolomics analysis showed that OCO fed pigs decreased the levels of serum alanine (P < 0.01), tryptophan (P < 0.05), carnosine (P < 0.01), and glutamic acid (P < 0.05), while the levels of threonine (P < 0.05) was increased compared to CCO. Moreover, consuming OCO decreased the hepatic metabolites from threonine catabolism pathways, including α-ketobutyrate (P < 0.01), α-amino-butyrate (P < 0.05), and propionic acid (P < 0.05), compared to CCO treatment. In addition, OCO increased hepatic NAD level by activating tryptophan-NAD+ metabolic pathway. Overall, OCO selectively modulated amino acid metabolism in nursery pigs, which may further affect growth performance.

scholarly journals Whole-Plant Corn Silage Improves Rumen Fermentation and Growth Performance of Beef Cattle by Altering Rumen Microbiota

2021 ◽  
Author(s):  
Yalei Cui ◽  
Hua Liu ◽  
Zimin Gao ◽  
Junying Xu ◽  
Boshuai Liu ◽  
...  
Keyword(s):  
Amino Acid ◽  
Beef Cattle ◽  
Growth Performance ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Rumen Fermentation ◽  
Corn Silage ◽  
Corn Straw ◽  
Rumen Microbiota ◽  
Whole Plant

Abstract BackgroundIn recent years, whole-plant corn silage had been widely used in China. Roughage was an important source of nutrition for ruminants and had an important effect on rumen microbiota, which plays an important role in animal growth performance and feed digestion. To better understand the effects of different silages on rumen microbiota, the effects of whole-plant corn silage or corn straw silage on growth performance, rumen fermentation products, and rumen microbiota of Simmental hybrid cattle were studied. Results60 healthy Simmental hybrid cattle were randomly divided into 2 groups with 6 repeats in each group and 5 cattle in each group. They were fed with whole-plant corn silage (WS) diet and corn straw silage (CS) diet respectively. Compared with corn straw silage, whole-plant corn silage significantly increased daily gain and decreased feed-weight ratio of beef cattle. Whole-plant corn silage also decreased the acetic acid in the rumen and the acetate to propionate (A/P) ratio compared with corn straw silage. At the genus level, the relative abundance of Prevotella_1 was significantly increased while the relative abundance of Succinivibrionaceae_UCG-002, Succiniclasticum, norank_f_Bacteroidales_RF16_group, and Ruminococcus_1 was decreased in cattle fed whole-plant corn silage compared with those fed corn straw silage. Prevotella_1 was positively correlated with acetic acid and A/P ratio, Succinivibrionaceae_UCG-002 was positively correlated with propionic acid and butyric acid, and negatively correlated with pH, Succiniclasticum was positively correlated with pH and A/P ratio, and norank_f__F082 and Rikenellaceae_RC9_gut_group were positively correlated with pH, propionic acid and butyric acid. Feeding whole-plant corn silage improved amino acid metabolism, nucleotide metabolism, and metabolism of beef cattle compared with feeding corn straw silage. Correlation analysis between rumen microbiota and metabolic pathways showed that Succinivibrionaceae_UCG-002 was negatively correlated with Carbohydrate Metabolism, Glycan Biosynthesis, and Metabolism, while Prevotellaceae_UCG-003 was positively correlated with Amino Acid Metabolism, and Carbohydrate Metabolism. ConclusionsFeeding whole-plant corn silage can improve the production performance rumen fermentation of beef cattle by altering rumen microbiota, amino acid metabolism, and nucleotide metabolism.

scholarly journals Liver Metabolome and Proteome Response of Turbot (Scophthalmus maximus) to Lysine and Leucine in Free and Dipeptide Forms

2021 ◽  
Vol 8 ◽  
Author(s):  
Yuliang Wei ◽  
Benxiang Li ◽  
Houguo Xu ◽  
Mengqing Liang
Keyword(s):  
Protein Synthesis ◽  
Amino Acid ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Scophthalmus Maximus ◽  
Protein Digestion ◽  
Metabolic Processes ◽  
Differential Proteins ◽  
Protein Synthesis And Degradation ◽  
Synthesis And Degradation

Omics approaches provide more metabolic information to explain the relationship between dietary nutrition and fish growth. This study aimed to explore the metabolome and proteome response of turbot (Scophthalmus maximus) fed diets containing lysine and leucine in free and dipeptide forms by the approaches of integrated liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based metabolomics and isobaric tags for relative and absolute quantification (iTRAQ)-based proteomics. Plant protein-based diets were formulated to contain the equivalent of lysine and leucine in free amino acid [crystalline amino acid (CAA)] and synthetic Lys-Leu (Lys-Leu) forms. The metabolome and proteome profiles of the liver were screened in fish fed either the CAA diet or the Lys-Leu diet after an 8-week feeding trial. Fish fed the Lys-Leu diet showed a significantly higher final body weight and a specific growth rate compared with fish fed the CAA diet. Protein- and amino acid-related metabolic processes in the liver were identified between the Lys-Leu and CAA groups based on differential metabolites and proteins. The proteolytic enzymes and amino acid transporters from differential proteins of the liver showed that the process of protein digestion and absorption may be affected by the different forms of lysine and leucine in the feed. A mechanistic target of rapamycin complex 1 and ubiquitin proteasome pathways were identified by differential proteins, which were involved in the processes of protein synthesis and degradation in the liver. Lysine degradation, tryptophan metabolism, alanine, aspartate, and glutamate metabolism, arginine biosynthesis, arginine and proline metabolism, and glycine, serine, and threonine metabolism were identified based on differential metabolites and proteins, which showed that the metabolism of various amino acids, including lysine, had been affected by both the CAA and Lys-Leu groups. In conclusion, the data of integrated metabonomics and proteomics suggested that different forms of lysine and leucine in the feed may affect liver metabolic processes including protein digestion and absorption, protein synthesis and degradation, and amino acid metabolism. In addition, a good correlation between differential metabolites and proteins was observed in amino acid metabolism by using the approaches of integrated LC-MS/MS-based metabolomics and iTRAQ-based proteomics.

Amino Acid Metabolism

1979 ◽  
Vol 7 (1) ◽  
pp. 261-262
Author(s):  
E. V. ROWSELL
Keyword(s):  
Amino Acid ◽  
Amino Acid Metabolism ◽  
Acid Metabolism
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