Preliminary time-course study of antiinflammatory macrophage infiltration in crush-injured skeletal muscle

Mitochondrial diseases are genetic disorders leading to an impaired mitochondrial function and resulting in exercise intolerance and muscle weakness. In patients, muscle fatigue due to defects in mitochondrial oxidative capacities commonly precedes muscle weakness. In mice, the fast-twitch skeletal muscle-specific Tfam deletion (Tfam KO) leads to deficit in the respiratory chain activity, severe muscle weakness and early death. Here, we performed a time-course study of mitochondrial and muscular dysfunctions in 11 and 14 weeks Tfam KO mice, i.e., before and when mice are about to enter the terminal stage, respectively. While force in the unfatigued state was reduced in Tfam KO mice as compared to control littermates (WT) only at 14 weeks, during repeated submaximal contractions fatigue was faster at both ages. During fatiguing stimulation, total phosphocreatine breakdown was larger in Tfam KO muscle than in WT muscle at both ages whereas phosphocreatine consumption was faster only at 14 weeks. In conclusion, the Tfam KO mouse model represents a reliable model of lethal mitochondrial myopathy where impaired mitochondrial energy production and premature fatigue occur before muscle weakness and early death.

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UPTAKE OF OXYTOCIN IN TISSUES AFTER INTRAVENOUS ADMINISTRATION OF TRITIATED OXYTOCIN IN RATS

Acta Endocrinologica ◽

10.1530/acta.0.0610432 ◽

1969 ◽

Vol 61 (3) ◽

pp. 432-440 ◽

Cited By ~ 7

Author(s):

Ingvar Sjöholm ◽

Gunnar Rydén

Keyword(s):

Skeletal Muscle ◽

Distribution Pattern ◽

Intravenous Injection ◽

Intravenous Administration ◽

Time Course ◽

Tritiated Water ◽

Preferential Distribution ◽

Time Period ◽

Initial Uptake

ABSTRACT The distribution of oxytocin in the kidneys, liver, uterus and skeletal muscle of the rat was followed during 10 min after intravenous injection of tritium labelled oxytocin. Oxytocin was found to be taken up and degraded mainly in the kidneys and the liver. After 150 seconds no intact oxytocin could be detected in these organs. The time course of the distribution of the radioactivity in the liver and the skeletal muscle showed no noteworthy characteristics, whereas a different course was found in the kidneys and in the uterus. In the kidneys, the radioactivity increased continuously from 60 to 200 seconds after the injection, indicating an accumulation of oxytocin or its metabolites in the kidneys. In the uterus a high initial uptake was observed, followed by a decrease of the radioactivity from 60 to 100 seconds after the injection. This distribution pattern was specific to oxytocin, since the uptake of tritiated tyrosine and tritiated water was almost constant during the same time period. These findings may indicate a preferential distribution of oxytocin to the uterus.

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Time course study of blood pressure in children over a three-year period. Bogalusa Heart Study

Hypertension ◽

10.1161/01.hyp.2.4.102 ◽

1980 ◽

Vol 2 (4) ◽

pp. 102-108 ◽

Cited By ~ 26

Author(s):

A. W. Voors ◽

L. S. Webber ◽

G. S. Berenson

Keyword(s):

Blood Pressure ◽

Time Course ◽

Bogalusa Heart Study ◽

Heart Study ◽

Time Course Study

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A Time-Course Comparison of Skeletal Muscle Metabolomic Alterations in Walker-256 Tumour-Bearing Rats at Different Stages of Life

Metabolites ◽

10.3390/metabo11060404 ◽

2021 ◽

Vol 11 (6) ◽

pp. 404

Author(s):

Gabriela de Matuoka e Chiocchetti ◽

Leisa Lopes-Aguiar ◽

Natália Angelo da Silva Miyaguti ◽

Lais Rosa Viana ◽

Carla de Moraes Salgado ◽

...

Keyword(s):

Skeletal Muscle ◽

Time Course ◽

Cancer Cachexia ◽

Tumour Bearing ◽

Walker 256 Tumour ◽

Walker 256 ◽

Muscle Changes ◽

The Right ◽

Biomarker Research ◽

Host Metabolism

Cancer cachexia is a severe wasting condition that needs further study to find ways to minimise the effects of damage and poor prognosis. Skeletal muscle is the most impacted tissue in cancer cachexia; thus, elucidation of its metabolic alterations could provide a direct clue for biomarker research and be applied to detect this syndrome earlier. In addition, concerning the significant changes in the host metabolism across life, this study aimed to compare the metabolic muscle changes in cachectic tumour-bearing hosts at different ages. We performed 1H-NMR metabolomics in the gastrocnemius muscle in weanling and young adult Walker-256 tumour-bearing rats at different stages of tumour evolution (initial, intermediate, and advanced). Among the 49 metabolites identified, 24 were significantly affected throughout tumour evolution and 21 were significantly affected regarding animal age. The altered metabolites were mainly related to increased amino acid levels and changed energetic metabolism in the skeletal muscle, suggesting an expressive catabolic process and diverted energy production, especially in advanced tumour stages in both groups. Moreover, these changes were more severe in weanling hosts throughout tumour evolution, suggesting the distinct impact of cancer cachexia regarding the host’s age, highlighting the need to adopting the right animal age when studying cancer cachexia.

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