scholarly journals Aspergillus fumigatus Hsp90 interacts with the main components of the cell wall integrity pathway and cooperates in heat shock and cell wall stress adaptation

2020 ◽  
Author(s):  
Marina Campos Rocha ◽  
Karine Minari ◽  
João Henrique Tadini Marilhano Fabri ◽  
Joshua D. Kerkaert ◽  
Lisandra Marques Gava ◽  
...  
Keyword(s):  
Cell Wall ◽  
Heat Shock ◽  
Aspergillus Fumigatus ◽  
Wall Stress ◽  
Cell Wall Integrity ◽  
Stress Adaptation ◽  
Cell Wall Stress ◽  
Cell Wall Integrity Pathway ◽  
Main Components

scholarly journals Author Correction: Signalling through the yeast MAPK Cell Wall Integrity pathway controls P-body assembly upon cell wall stress

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Raúl García ◽  
Verónica Pulido ◽  
Sara Orellana-Muñoz ◽  
César Nombela ◽  
Carlos R. Vázquez de Aldana ◽  
...  
Keyword(s):  
Cell Wall ◽  
Wall Stress ◽  
Cell Wall Integrity ◽  
Cell Wall Stress ◽  
Cell Wall Integrity Pathway

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

scholarly journals Signalling through the yeast MAPK Cell Wall Integrity pathway controls P-body assembly upon cell wall stress

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Raúl García ◽  
Verónica Pulido ◽  
Sara Orellana-Muñoz ◽  
César Nombela ◽  
Carlos R. Vázquez de Aldana ◽  
...  
Keyword(s):  
Cell Wall ◽  
Wall Stress ◽  
Cell Wall Integrity ◽  
Cell Wall Stress ◽  
Cell Wall Integrity Pathway

scholarly journals The Heat Shock Transcription Factor HsfA Is Essential for Thermotolerance and Regulates Cell Wall Integrity in Aspergillus fumigatus

2021 ◽  
Vol 12 ◽  
Author(s):  
João Henrique Tadini Marilhano Fabri ◽  
Marina Campos Rocha ◽  
Caroline Mota Fernandes ◽  
Gabriela Felix Persinoti ◽  
Laure Nicolas Annick Ries ◽  
...  
Keyword(s):  
Cell Wall ◽  
Transcription Factor ◽  
Heat Shock ◽  
Aspergillus Fumigatus ◽  
Fungal Pathogens ◽  
Elevated Temperatures ◽  
Fungal Infections ◽  
Wall Stress ◽  
High Temperature Stress ◽  
Cell Wall Integrity

The deleterious effects of human-induced climate change have long been predicted. However, the imminent emergence and spread of new diseases, including fungal infections through the rise of thermotolerant strains, is still neglected, despite being a potential consequence of global warming. Thermotolerance is a remarkable virulence attribute of the mold Aspergillus fumigatus. Under high-temperature stress, opportunistic fungal pathogens deploy an adaptive mechanism known as heat shock (HS) response controlled by heat shock transcription factors (HSFs). In eukaryotes, HSFs regulate the expression of several heat shock proteins (HSPs), such as the chaperone Hsp90, which is part of the cellular program for heat adaptation and a direct target of HSFs. We recently observed that the perturbation in cell wall integrity (CWI) causes concomitant susceptibility to elevated temperatures in A. fumigatus, although the mechanisms underpinning the HS response and CWI cross talking are not elucidated. Here, we aim at further deciphering the interplay between HS and CWI. Our results show that cell wall ultrastructure is severely modified when A. fumigatus is exposed to HS. We identify the transcription factor HsfA as essential for A. fumigatus viability, thermotolerance, and CWI. Indeed, HS and cell wall stress trigger the coordinated expression of both hsfA and hsp90. Furthermore, the CWI signaling pathway components PkcA and MpkA were shown to be important for HsfA and Hsp90 expression in the A. fumigatus biofilms. Lastly, RNA-sequencing confirmed that hsfA regulates the expression of genes related to the HS response, cell wall biosynthesis and remodeling, and lipid homeostasis. Our studies collectively demonstrate the connection between the HS and the CWI pathway, with HsfA playing a crucial role in this cross-pathway regulation, reinforcing the importance of the cell wall in A. fumigatus thermophily.

scholarly journals A novel connection between the Cell Wall Integrity and the PKA pathways regulates cell wall stress response in yeast

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Raúl García ◽  
Enrique Bravo ◽  
Sonia Diez-Muñiz ◽  
Cesar Nombela ◽  
Jose M. Rodríguez-Peña ◽  
...  
Keyword(s):  
Cell Wall ◽  
Stress Response ◽  
Wall Stress ◽  
Cell Wall Integrity ◽  
Cell Wall Stress

scholarly journals KRE5 Suppression Induces Cell Wall Stress and Alternative ER Stress Response Required for Maintaining Cell Wall Integrity in Candida glabrata

PLoS ONE ◽  
2016 ◽  
Vol 11 (8) ◽  
pp. e0161371 ◽  
Author(s):  
Yutaka Tanaka ◽  
Masato Sasaki ◽  
Fumie Ito ◽  
Toshio Aoyama ◽  
Michiyo Sato-Okamoto ◽  
...  
Keyword(s):  
Cell Wall ◽  
Stress Response ◽  
Er Stress ◽  
Candida Glabrata ◽  
Wall Stress ◽  
Cell Wall Integrity ◽  
Er Stress Response ◽  
Cell Wall Stress

scholarly journals The Transcription Factor SomA Synchronously Regulates Biofilm Formation and Cell Wall Homeostasis in Aspergillus fumigatus

mBio ◽  
2020 ◽  
Vol 11 (6) ◽  
Author(s):  
Yuan Chen ◽  
Francois Le Mauff ◽  
Yan Wang ◽  
Ruiyang Lu ◽  
Donald C. Sheppard ◽  
...  
Keyword(s):  
Cell Wall ◽  
Transcription Factor ◽  
Aspergillus Fumigatus ◽  
Biofilm Formation ◽  
Wall Stress ◽  
Fungal Cell ◽  
Glucan Synthase ◽  
Content Type ◽  
Cell Wall Stress ◽  
Chitin Synthases

ABSTRACT Polysaccharides are key components of both the fungal cell wall and biofilm matrix. Despite having distinct assembly and regulation pathways, matrix exopolysaccharide and cell wall polysaccharides share common substrates and intermediates in their biosynthetic pathways. It is not clear, however, if the biosynthetic pathways governing the production of these polysaccharides are cooperatively regulated. Here, we demonstrate that cell wall stress promotes production of the exopolysaccharide galactosaminogalactan (GAG)-depend biofilm formation in the major fungal pathogen of humans Aspergillus fumigatus and that the transcription factor SomA plays a crucial role in mediating this process. A core set of SomA target genes were identified by transcriptome sequencing and chromatin immunoprecipitation coupled to sequencing (ChIP-Seq). We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA. Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase. Thus, SomA plays a direct regulation of both GAG and cell wall polysaccharide biosynthesis. Consistent with these findings, SomA is required for the maintenance of normal cell wall architecture and compositions in addition to its function in biofilm development. Moreover, SomA was found to globally regulate glucose uptake and utilization, as well as amino sugar and nucleotide sugar metabolism, which provides precursors for polysaccharide synthesis. Collectively, our work provides insight into fungal adaptive mechanisms in response to cell wall stress where biofilm formation and cell wall homeostasis were synchronously regulated. IMPORTANCE The cell wall is essential for fungal viability and is absent from human hosts; thus, drugs disrupting cell wall biosynthesis have gained more attention. Caspofungin is a member of a new class of clinically approved echinocandin drugs to treat invasive aspergillosis by blocking β-1,3-glucan synthase, thus damaging the fungal cell wall. Here, we demonstrate that caspofungin and other cell wall stressors can induce galactosaminogalactan (GAG)-dependent biofilm formation in the human pathogen Aspergillus fumigatus. We further identified SomA as a master transcription factor playing a dual role in both biofilm formation and cell wall homeostasis. SomA plays this dual role by direct binding to a conserved motif upstream of GAG biosynthetic genes and genes involved in cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase. Collectively, these findings reveal a transcriptional control pathway that integrates biofilm formation and cell wall homeostasis and suggest SomA as an attractive target for antifungal drug development.

scholarly journals The High Osmotic Response and Cell Wall Integrity Pathways Cooperate to Regulate Transcriptional Responses to Zymolyase-induced Cell Wall Stress inSaccharomyces cerevisiae

2009 ◽  
Vol 284 (16) ◽  
pp. 10901-10911 ◽  
Author(s):  
Raúl García ◽  
Jose M. Rodríguez-Peña ◽  
Clara Bermejo ◽  
César Nombela ◽  
Javier Arroyo
Keyword(s):  
Cell Wall ◽  
Wall Stress ◽  
Cell Wall Integrity ◽  
Transcriptional Responses ◽  
Cell Wall Stress ◽  
Osmotic Response

scholarly journals Cell wall integrity and high osmolarity glycerol pathways are required for adaptation of Alternaria brassicicola to cell wall stress caused by brassicaceous indolic phytoalexins

2010 ◽  
Vol 13 (1) ◽  
pp. 62-80 ◽  
Author(s):  
Aymeric Joubert ◽  
Nelly Bataille-Simoneau ◽  
Claire Campion ◽  
Thomas Guillemette ◽  
Piétrick Hudhomme ◽  
...  
Keyword(s):  
Cell Wall ◽  
Wall Stress ◽  
Cell Wall Integrity ◽  
Alternaria Brassicicola ◽  
High Osmolarity ◽  
High Osmolarity Glycerol ◽  
Cell Wall Stress
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