Intragraft vasculitis and gene expression analysis: Association with acute rejection and prediction of mortality in long-term heart transplantation

2018 ◽  
Vol 32 (10) ◽  
pp. e13373
Author(s):  
Hui Tzu Lin-Wang ◽  
Reginaldo Cipullo ◽  
João Italo Dias França ◽  
Marco Aurelio Finger ◽  
Joao Manoel Rossi Neto ◽  
...  
Keyword(s):  
Gene Expression ◽  
Acute Rejection ◽  
Heart Transplantation ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Prediction Of Mortality

scholarly journals Identification of Appropriate Housekeeping Genes for Gene Expression Analysis in Long-term Hypoxia-treated Kidney Cells

2017 ◽  
Vol 6 (1) ◽  
pp. 15 ◽  
Author(s):  
Yousof Gheisari ◽  
Shiva Moein ◽  
ShaghayeghHaghjooy Javanmard ◽  
Maryam Abedi ◽  
MohammadHosein Izadpanahi
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Housekeeping Genes ◽  
Kidney Cells

scholarly journals In Utero Exposure to the Endocrine Disruptor Di-(2-Ethylhexyl) Phthalate Induces Long-Term Changes in Gene Expression in the Adult Male Adrenal Gland

Endocrinology ◽  
2014 ◽  
Vol 155 (5) ◽  
pp. 1667-1678 ◽  
Author(s):  
D.B. Martinez-Arguelles ◽  
E. Campioli ◽  
C. Lienhart ◽  
J. Fan ◽  
M. Culty ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Gene Expression Analysis ◽  
In Utero ◽  
In Utero Exposure ◽  
Long Term Effects ◽  
Ethylhexyl Phthalate ◽  
Dehp Exposure

The plasticizer di-(2-ethylhexyl) phthalate (DEHP) is used to add flexibility to polyvinylchloride polymers and as a component of numerous consumer and medical products. DEHP and its metabolites have been detected in amniotic fluid and umbilical cord blood, suggesting fetal exposure. In the present study, we used an in utero exposure model in which pregnant rat dams were exposed to 1- to 300-mg DEHP/kg·d from gestational day 14 until birth. We previously reported that this window of exposure to environmentally relevant doses of DEHP resulted in reduced levels of serum testosterone and aldosterone in adult male offspring and that the effects on aldosterone were sustained in elderly rats and resulted in decreased blood pressure. Here, we characterized the long-term effects of in utero DEHP exposure by performing global gene expression analysis of prepubertal (postnatal d 21) and adult (postnatal d 60) adrenal glands. We found that the peroxisome proliferator-activated receptor and lipid metabolism pathways were affected by DEHP exposure. Expression of 2 other DEHP targets, hormone-sensitive lipase and phosphoenolpyruvate carboxykinase 1 (Pck1), correlated with reduced aldosterone levels and may account for the inhibitory effect of DEHP on adrenal steroid formation. The angiotensin II and potassium pathways were up-regulated in response to DEHP. In addition, the potassium intermediate/small conductance calcium-activated channel Kcnn2 and 2-pore-domain potassium channel Knck5 were identified as DEHP targets. Based on this gene expression analysis, we measured fatty acid-binding protein 4 and phosphoenolpyruvate carboxykinase 1 in sera from control and DEHP-exposed rats and identified both proteins as putative serum biomarkers of in utero DEHP exposure. These results shed light on molecular targets that mediate DEHP long-term effects and, in doing so, provide means by which to assess past DEHP exposure.

scholarly journals SAT-725 Alterations in the Phenotype and Epigenomic Landscape of Luminal Breast Cancer Following Long-Term Nanomolar Exposure to Bisphenol a (BPA)

2020 ◽  
Vol 4 (Supplement_1) ◽  
Author(s):  
Jason Tan Liwag ◽  
Michael Cu Velarde ◽  
Pia Dano Bagamasbad
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Bisphenol A ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Stem Cell Population ◽  
Luminal Breast Cancer ◽  
Positive Contribution ◽  
Mcf7 Cells

Abstract Breast cancer (BCa) is the leading cause of female cancer-related death worldwide. Luminal BCa accounts for at least 70% of all BCa and is characterized by its hormone dependence, particularly to estrogen. Endocrine disruptors may function to exert the pro-tumorigenic effects of estrogen in proxy following environmental exposure. Bisphenol A (BPA) is a ubiquitous endocrine disruptor shown to increase risk towards developing BCa in multiple in vivo mammalian models and in vitro human breast models. Despite this, there is limited information on the phenotypic and epigenetic effects of nanomolar BPA following long-term exposure particularly in the context of BCa stem cell population, which accumulate the ‘cellular insult’ and are likely to pass such information down through epigenetic mechanisms. We hypothesize that BPA affects the epigenome, in part, by altering the regulation of DNA methyltransferases (DNMTs) since BPA disrupts transcriptional and translational regulation of DNMTs in various other tissue types such as the brain, testis and prostate. In this study, MCF7 cells were chronically (>4 weeks) cultured with nanomolar doses of BPA, and subsequently subjected to phenotypic assays and gene expression analysis in monolayer or mammosphere culture. Gene expression analysis revealed a downregulation of DNMT3A and ESR1 following long-term exposure to BPA in mammosphere culture of MCF7 cells, but not in monolayer culture. This is accompanied by alterations in mammosphere morphology, reduction in mammosphere size, an increase in the mammosphere number, and an increase in the CD49f+ population, indicating a positive contribution of BPA to stemness. However, there are no significant changes in proliferation, apoptotic rate, and metastasis in both monolayer and mammosphere culture. Future analysis includes assessment of genome-wide alterations in DNA methylation patterns in these chronically exposed MCF7 mammospheres, as well as identifying estrogen responsiveness, chemotherapeutic response, and self-renewal properties of these cancer cells.

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