An audit of liquid‐based cytology samples reported as high‐risk human papillomavirus and borderline nuclear change in endocervical cells

The objective of this study was to compare the sensitivity and specificity of a new method for self-sampling for high risk human papillomavirus (HPV) with direct sampling and liquid based cervical cytology. In Shanxi Province, China, 8,497 women (ages 27–56) underwent a self-sample for HPV using a conical-shaped brush placed into the upper vagina and rotated. Three to sixteen months later the women were screened with liquid-based cytology and direct HPV tests. Subjects with any abnormal test underwent colposcopy and multiple biopsies. Mean age was 40.9 years. 4.4 percent of subjects had ≥CIN II, 26% a positive self-sample and 24% a positive direct test for HPV. The sensitivity for detection of ≥CIN II was 87.5% for self-sampling, and 96.8% for the direct test (P < 0.001). The specificity was 77.2% for the self-sample and 79.7% for the direct test. With an abnormal Pap defined as ASCUS or greater the sensitivity of the Pap for the detection of ≥ CIN II was 88.3% and the specificity was 81.2%. We conclude that self-sampling for HPV is less sensitive for ≥ CIN II than the direct test, but similar to liquid based cytology.

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Novaprep® Vial Test is a suitable liquid-based cytology medium for high risk human papillomavirus testing by Hybrid Capture 2

Journal of Clinical Virology ◽

10.1016/j.jcv.2010.08.014 ◽

2010 ◽

Vol 49 (4) ◽

pp. 286-289 ◽

Cited By ~ 3

Author(s):

Jean-Luc Prétet ◽

Chrystelle Vidal ◽

Karine Le Bail Carval ◽

Rajeev Ramanah ◽

Xavier Carcopino ◽

...

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

Hybrid Capture ◽

Human Papillomavirus Testing ◽

Hybrid Capture 2 ◽

Liquid Based Cytology

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Detection of human papillomavirus oncoprotein E7 in liquid-based cytology

Journal of General Virology ◽

10.1099/vir.0.034884-0 ◽

2012 ◽

Vol 93 (2) ◽

pp. 356-363 ◽

Cited By ~ 4

Author(s):

Maria Lidqvist ◽

Olle Nilsson ◽

Jan Holmgren ◽

Sebastian Hölters ◽

Eva Röijer ◽

...

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

Low Risk ◽

Transformed Cells ◽

Specific Staining ◽

Hpv E7 ◽

Detection And Identification ◽

Liquid Based Cytology ◽

E7 Proteins ◽

High Risk Hpv

The selection and characterization of a set of mouse mAbs against high-risk human papillomavirus (HPV) E7 oncoprotein and the development of protocols for immunocytochemistry (ICC) are described here. A large number of antibodies raised towards HPV16 and 18 E7 were tested for high-risk specificity by ELISA using a panel of HPV E7 proteins. Antibodies detecting low-risk E7 were discarded, resulting in 38 high-risk HPV E7-specific antibodies. The corresponding epitopes were mapped using overlapping HPV E7 fragments displayed on phage particles. Functionality in ICC against formalin-fixed cervical cancer cell lines was demonstrated for ten mAbs; their high-risk specificity was confirmed by Western blot analysis and ICC on transiently transformed cells expressing high- or low-risk HPV E7. These mAbs were specific for one or several of the high-risk strains HPV16, 18, 31, 35 and 45. Specific E7 staining of liquid-based cytology (LBC) samples was demonstrated for seven mAbs and optimized protocols were established. The E716-41 and E718-79 mAbs demonstrated particularly strong and specific staining of cells stored in LBC fluid for at least 6 months. It is proposed that the high-risk HPV E7 staining protocols established in this study may have the potential to be included in a complementary test for the detection and identification of malignantly transformed cells, in for example atypical squamous cells of undetermined significance samples.

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Follow-up outcomes for a large cohort of US women with negative imaged liquid-based cytology findings and positive high risk human papillomavirus test results

Gynecologic Oncology ◽

10.1016/j.ygyno.2011.04.037 ◽

2011 ◽

Vol 122 (2) ◽

pp. 291-296 ◽

Cited By ~ 19

Author(s):

Chengquan Zhao ◽

Xiangbai Chen ◽

Agnieszka Onisko ◽

Anisa Kanbour ◽

R. Marshall Austin

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

Large Cohort ◽

Test Results ◽

Liquid Based Cytology

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Clinical laboratory evaluation of Invader® chemistry and hybrid capture for detection of high-risk human papillomavirus in liquid-based cytology specimens

Diagnostic Microbiology and Infectious Disease ◽

10.1016/j.diagmicrobio.2011.07.004 ◽

2011 ◽

Vol 71 (3) ◽

pp. 230-235 ◽

Cited By ~ 8

Author(s):

Erik Munson ◽

Brian K. Du Chateau ◽

Bronya Bellerose ◽

Jolanta Czarnecka ◽

Judy Griep

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

Clinical Laboratory ◽

Laboratory Evaluation ◽

Hybrid Capture ◽

Liquid Based Cytology

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Сontroversial issues in cytological screening for cervical cancer (literature review)

Tumors of female reproductive system ◽

10.17650/1994-4098-2020-16-2-63-71 ◽

2020 ◽

Vol 16 (2) ◽

pp. 63-71

Author(s):

V. I. Novik

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

High Risk ◽

Literature Review ◽

Staff Training ◽

Controversial Issues ◽

Organizational Issues ◽

Liquid Based Cytology ◽

Automated Screening ◽

Screening Systems

This article discusses the controversial issues of cytological screening for cervical cancer, including the use of liquid-based cytology and automated screening systems, high-risk human papillomavirus testing, and organizational issues of screening and staff training.

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Primary cervical screening with high risk human papillomavirus testing: observational study

BMJ ◽

10.1136/bmj.l240 ◽

2019 ◽

pp. l240 ◽

Cited By ~ 33

Author(s):

Matejka Rebolj ◽

Janet Rimmer ◽

Karin Denton ◽

John Tidy ◽

Christopher Mathews ◽

...

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

High Risk ◽

Observational Study ◽

Cervical Intraepithelial Neoplasia ◽

Cervical Screening ◽

Intraepithelial Neoplasia ◽

Cervical Intraepithelial Neoplasia Grade ◽

Liquid Based Cytology ◽

Grade 3

AbstractObjectiveTo provide the first report on the main outcomes from the prevalence and incidence rounds of a large pilot of routine primary high risk human papillomavirus (hrHPV) testing in England, compared with contemporaneous primary liquid based cytology screening.DesignObservational study.SettingThe English Cervical Screening Programme.Participants578 547 women undergoing cervical screening in primary care between May 2013 and December 2014, with follow-up until May 2017; 183 970 (32%) were screened with hrHPV testing.InterventionsRoutine cervical screening with hrHPV testing with liquid based cytology triage and two early recalls for women who were hrHPV positive and cytology negative, following the national screening age and interval recommendations.Main outcome measuresFrequency of referral for a colposcopy; adherence to early recall; and relative detection of cervical intraepithelial neoplasia grade 2 or worse from hrHPV testing compared with liquid based cytology in two consecutive screening rounds.ResultsBaseline hrHPV testing and early recall required approximately 80% more colposcopies, (adjusted odds ratio 1.77, 95% confidence interval 1.73 to 1.82), but detected substantially more cervical intraepithelial neoplasia than liquid based cytology (1.49 for cervical intraepithelial neoplasia grade 2 or worse, 1.43 to 1.55; 1.44 for cervical intraepithelial neoplasia grade 3 or worse, 1.36 to 1.51) and for cervical cancer (1.27, 0.99 to 1.63). Attendance at early recall and colposcopy referral were 80% and 95%, respectively. At the incidence screen, the 33 506 women screened with hrHPV testing had substantially less cervical intraepithelial neoplasia grade 3 or worse than the 77 017 women screened with liquid based cytology (0.14, 0.09 to 0.23).ConclusionsIn England, routine primary hrHPV screening increased the detection of cervical intraepithelial neoplasia grade 3 or worse and cervical cancer by approximately 40% and 30%, respectively, compared with liquid based cytology. The very low incidence of cervical intraepithelial neoplasia grade 3 or worse after three years supports extending the screening interval.

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