scholarly journals Evaluation of a pelleted diet for larval mass‐rearing of Anastrepha ludens and Anastrepha obliqua

2020 ◽  
Vol 168 (6-7) ◽  
pp. 502-512 ◽  
Author(s):  
Marysol Aceituno‐Medina ◽  
José Pedro Rivera‐Ciprian ◽  
Emilio Hernández
Keyword(s):  
Mass Rearing ◽  
Anastrepha Ludens ◽  
Anastrepha Obliqua ◽  
Pelleted Diet

When Less Is More: Sex Ratios for the Mass-Rearing of Anastrepha ludens (Diptera: Tephritidae)

2019 ◽  
Author(s):  
G I González-López ◽  
E Solís-Echeverría ◽  
F Díaz-Fleischer ◽  
D Pérez-Staples
Keyword(s):  
Sex Ratio ◽  
Egg Production ◽  
Sex Ratios ◽  
Pupal Stage ◽  
Mass Rearing ◽  
Anastrepha Ludens ◽  
Adult Density ◽  
Genetic Sexing ◽  
Male Harassment ◽  
Less Is More

Abstract Anastrepha ludens (Loew) is one of the most important pests of citrus and mango crops in Mexico. A method used to control this pest is the sterile insect technique, which consists in the mass production, irradiation, and release of insects in affected areas. The production of insects begins with the establishment of colonies to produce eggs, which must be highly fertile to ensure an adequate production of larvae. However, female fecundity and fertility can be affected by adult density and sex ratio, thus an optimal sex ratio in mass-rearing cages must be used. The genetic sexing strain of A. ludens (Tapachula-7) allows the identification of the sex at the pupal stage, making it possible to establish rearing cages with different sex ratios. We determined if different sex ratios have an effect on egg production. Two sex ratios (4♀: 1♂ and 1♀: 1♂) were compared. Fecundity, fertility and survival at different ages were also determined. Higher fertility and fecundity per female were observed at a ratio of 4:1. However, females with higher fecundity had reduced survival probabilities. In conclusion, maintaining colonies with a lower proportion of males in cages ensures a greater fecundity and fertility. Further research is necessary to understand whether results can be attributed to lower male harassment in cages.

Mass Rearing History Negatively Affects Mating Success of Male Anastrepha ludens (Diptera: Tephritidae) Reared for Sterile Insect Technique Programs

2005 ◽  
Vol 98 (5) ◽  
pp. 1510-1516 ◽  
Author(s):  
Juan Rull ◽  
Odette Brunel ◽  
Maria Elena Mendez
Keyword(s):  
Mating Success ◽  
Sterile Insect Technique ◽  
Mass Rearing ◽  
Anastrepha Ludens

Pre- and Post-Copulatory Competitiveness of the Genetic Sexing Strain Tapachula-7 of Anastrepha ludens (Diptera: Tephritidae)

2020 ◽  
Vol 113 (5) ◽  
pp. 2163-2170
Author(s):  
Yair Contreras-Navarro ◽  
Diana Pérez-Staples ◽  
Dina Orozco-Dávila ◽  
Francisco Díaz-Fleischer
Keyword(s):  
Sterile Insect Technique ◽  
Copulatory Behavior ◽  
Mass Rearing ◽  
Economic Importance ◽  
Anastrepha Ludens ◽  
Sexual Performance ◽  
Field Cage ◽  
Wild Female ◽  
Genetic Sexing ◽  
Genetic Sexing Strain

Abstract Anastrepha ludens (Loew) (Diptera: Tephritidae) is an endemic pest of Mexico, attacking several fruits of economic importance. The Sterile Insect Technique (SIT), consisting of mass-rearing, irradiation and release of adults, is used to control this pest in affected areas. Currently, a genetic sexing strain (Tapachula 7, Tap-7) consisting only of A. ludens males is being released, yet we lack information on its sexual performance at the early ages when they are released and on its post copulatory behavior in terms of sperm transfer and mating inhibition. Here, sexual competitiveness at young ages and ability to inhibit female re-mating was compared between sterile Tap-7, Standard Bisexual (SB), or wild males both in laboratory and field cage conditions. Sperm stored by females mating with wild, Tap-7, or SB strain males was also compared. Six-day-old sterile Tap-7 males had low mating rates, however, by 7 d of age 80% of males had mated. Tap-7 males were just as likely as wild males to inhibit wild female re-mating. In field cages, sterile Tap-7 males mated faster than wild and sterile males from the SB strain and had comparable mating success to wild males. Females mated to sterile Tap-7 males stored more sperm than those mated to sterile SB males. Females mated to wild males stored more sperm than females mated to any other type of male. Mass-rearing had a greater effect on decreasing sperm stored by females than irradiation. We recommend continuing the release of the GSS strain of A. ludens.

Antipredator behavior of the new mass-reared unisexual strain of the Mexican Fruit Fly

2015 ◽  
Vol 106 (3) ◽  
pp. 314-321 ◽  
Author(s):  
G. I. González-López ◽  
D. Rao ◽  
F. Díaz-Fleischer ◽  
D. Orozco-Dávila ◽  
D. Pérez-Staples
Keyword(s):  
Antipredator Behavior ◽  
Fruit Fly ◽  
Mass Rearing ◽  
Quality Parameters ◽  
Quality Parameter ◽  
Anastrepha Ludens ◽  
Activity Levels ◽  
Mexican Fruit Fly ◽  
Lower Survival ◽  
Baseline Activity

AbstractTephritid pests controlled through the sterile insect technique (SIT) are mass-reared and subsequently released in affected areas. Several quality parameters are currently used to test adults, but none take into account interactions with a predator. When sterile males are released in the field, they will need to avoid predators until they reach sexual maturity and survive long enough to mate with wild females. Spiders are one of the most common predators that flies may encounter in release sites. In this study, we evaluated the antipredator behavior of a mass-reared sterile unisexual strain (‘Tapachula-7’) of the Mexican fruit fly Anastrepha ludens (Diptera: Tephritidae) against their spider predators. We sampled spiders in citrus trees to determine which families could be more common. We established the baseline activity rates of sterile Tapachula-7 (Tap-7) flies in comparison with wild flies. We also tested the behavior of the fertile and sterile bisexual strain and wild flies against hunting spiders (Family Salticidae) and orb building spiders (Family Tetragnathidae). We recorded 18 spider families, with Salticidae being the most dominant. Tap-7 flies diminished their activity in comparison with wild males at 1800 h but showed similar activity levels earlier in the day. When exposed to orb-web spiders (Leucauge venusta), Tap-7, fertile and sterile males from the bisexual strain had similar rates of survival, but Tap-7 males showed lower survival than wild males. Against hunting spiders (Phidippus audax), wild males had higher probability of defensive wing displays, but there was no difference in spider attack rates. In general, sterile Tap -7 males performed as well as males from the bisexual strain, although they had lower survival than wild males. This could be due to either mass-rearing and/or irradiation effects. We recommend the use of the defensive wing display behavior as a quality parameter and propose a rapid and effective method to evaluate fly activity. The efficiency of SIT will be improved if released sterile males have the same antipredator repertoire as their wild counterparts.

scholarly journals McPhail Trap Captures of Anastrepha obliqua and Anastrepha ludens (Diptera: Tephritidae) in Relation to Time of Day

1994 ◽  
Vol 77 (2) ◽  
pp. 290 ◽  
Author(s):  
Edi A. Malo ◽  
Georgina I. Zapien
Keyword(s):  
Time Of Day ◽  
Anastrepha Ludens ◽  
Anastrepha Obliqua ◽  
Mcphail Trap

scholarly journals DISPERSAL AND LONGEVITY OF WILD AND MASS-REARED ANASTREPHA LUDENS AND ANASTREPHA OBLIQUA (DIPTERA: TEPHRITIDAE)

2007 ◽  
Vol 90 (1) ◽  
pp. 123-135 ◽  
Author(s):  
Emilio Hernández ◽  
Dina Orozco ◽  
Salvador Flores Breceda ◽  
Julio Domínguez
Keyword(s):  
Anastrepha Ludens ◽  
Anastrepha Obliqua

scholarly journals Polyandrous Mexican Fruit Flies: Second Male Paternity and Biological Attributes of Transgenic Strains

Insects ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 5
Author(s):  
Betzabé Verónica-Murrieta ◽  
José Salvador Meza ◽  
Martha Lucía Baena ◽  
Gerardo Alvarado-Castillo ◽  
Diana Pérez-Staples
Keyword(s):  
Sterile Insect Technique ◽  
Mass Rearing ◽  
Larval Survival ◽  
Anastrepha Ludens ◽  
Marker Genes ◽  
Fluorescent Marker ◽  
Significant Difference ◽  
Lower Ability ◽  
Biological Attributes ◽  
Transgenic Strains

Anastrepha ludens (Diptera: Tephritidae), is a damaging agricultural pest. Currently, the Sterile Insect Technique (SIT) is used as part of its control. The SIT consists of the mass-rearing, sterilization, and release of insects in target areas. Sterile males mate with wild females, and prevent them from laying fertile eggs. However, even if females mate with sterile males, they can then remate with a second male. If this second male is wild, then this could reduce the efficiency of the SIT by producing viable offspring. The amount of progeny produced by second males (P2 values) for A. ludens is unknown. Here, we evaluated the biological attributes, mating competitiveness, and the proportion of male paternity gained by the second male, using strains that carry fluorescent marker genes and can be potentially used to develop transgenic sexing strains. Furthermore, the transgenic strains were irradiated, to test their ability to induce sterility in females. We found that the 443-G strain had significantly higher larval survival than the 419-R strain. No significant difference was found between the two strains in their mating probability with wild females. We found P2 values between 67 and 74% for the 419-R and the 443-G strain, respectively. Second male sperm precedence only decreased slightly after 12 days, suggesting that sperm from the first and second male is not mixing with time, but rather the second male’s sperm prevails. Furthermore, sterile 443-G males induced significantly higher sterility in females than sterile males from the 419-R strain. The apparent lower ability of the 443-G strain to inhibit female remating should be further investigated. Knowledge of the pre and postcopulatory performance of transgenic strains will help in understanding their potential for control.

scholarly journals Notas sobre la biología y ecología de Anastrepha obliqua (Diptera: Tephritidae), plaga de plantas anarcardiaceas en América tropical. I. Formas inmaduras.

2016 ◽  
Vol 8 (2) ◽  
pp. 116 ◽  
Author(s):  
Julia Soto-Manitiu ◽  
Luis G. Chaverri ◽  
Luis Fernando Jirón
Keyword(s):  
Small Groups ◽  
Mass Rearing ◽  
Biological Cycle ◽  
Larval Population ◽  
Minute Period ◽  
Anastrepha Obliqua ◽  
Oviposition Substrate ◽  
Fallen Fruit

Some details on the biology, behavior and laboratory mass rearing of Anastrepha obliqua are offered. Information on larvaI diets and oviposition substrates are discussed. Eggs of A. obliqua are, very succeptible to dehydratation and they collapse just few minutes after oviposition, if substrate for oviposition is not near 100% R.H. When using fruits as oviposition substrate, smallerfruit species, Spanish plums (Spondias spp.) offer higher yields. Bigger fruits (mango) loose large amounts of water and it accumulates in the sand substrate drowning mature larvae. After analyzing the biological cycle, the weaker part seems to be the 20 minute period in which mature larvae abandon the fallen fruit substrate and crawl few a centimeters on the floor seeking for an appropiate place for ovipositing. In this period large amounts of predators easily diminish larval population. Once pupation takes place, adult forms hatch in small groups, after eleven days, when atmosferic relativehumidity reaches 70%. Another observations are also included.

scholarly journals Nonhost Status of Commercial Persea americana ‘Hass’ to Anastrepha ludens, Anastrepha obliqua, Anastrepha serpentina, and Anastrepha striata (Diptera: Tephritidae) in Mexico

2004 ◽  
Vol 97 (2) ◽  
pp. 293-309 ◽  
Author(s):  
Martín Aluja ◽  
Francisco Díaz-Fleischer ◽  
José Arredondo
Keyword(s):  
Persea Americana ◽  
Anastrepha Ludens ◽  
Anastrepha Obliqua

Generic Irradiation and Hot Water Phytosanitary Treatments for Mango Fruits cv. ‘Ataulfo’ niño Infested by Anastrepha ludens and Anastrepha obliqua (Diptera: Tephritidae)

2018 ◽  
Vol 111 (5) ◽  
pp. 2110-2119 ◽  
Author(s):  
Emilio Hernández ◽  
Marysol Aceituno-Medina ◽  
Jorge Toledo ◽  
Yeudiel Gómez-Simuta ◽  
Juan Manuel Villarreal-Fuentes ◽  
...  
Keyword(s):  
Hot Water ◽  
Anastrepha Ludens ◽  
Anastrepha Obliqua
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